Value of serial quantification of fungal DNA by a real-time PCR-based technique for early diagnosis of invasive aspergillosis in patients with febrile neutropenia

Journal of Clinical Microbiology

Volumn 47, Issue 2, 2009, Pages 379-384

  Cuenca Estrella, Manuel ^a   Meije, Yolanda ^b   Diaz Pedroche, Carmen ^b   Gomez Lopez, Alicia ^a   Buitrago, Maria J ^a   Bernal Martinez, Leticia ^a   Grande, Carlos ^b   San Juan, Rafael ^b   Lizasoain, Manuel ^b   Rodriguez Tudela, Juan L ^a   Aguado, Jose M ^b  

^a INSTITUTO DE SALUD CARLOS III   (Spain)

^b HOSPITAL UNIVERSITARIO 12 DE OCTUBRE   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; GALACTOMANNAN; MANNAN;

ADULT; ARTICLE; ASPERGILLOSIS; ASPERGILLUS; CONTROLLED STUDY; DNA DETERMINATION; EARLY DIAGNOSIS; FALSE POSITIVE RESULT; FEBRILE NEUTROPENIA; FEMALE; FUNGAL DETECTION; HIGH RESOLUTION COMPUTER TOMOGRAPHY; HUMAN; INFECTION RISK; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PREDICTIVE VALIDITY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROLOGY; BLOOD; EVALUATION; FEVER; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; NEUTROPENIA; PREDICTION AND FORECASTING; ROC CURVE; SERUM; THORAX RADIOGRAPHY; TIME;

ASPERGILLUS; ASPERGILLUS SP.;

ASPERGILLOSIS; ASPERGILLUS; BLOOD; DNA, FUNGAL; EARLY DIAGNOSIS; FEMALE; FEVER; HUMANS; MALE; MANNANS; MIDDLE AGED; NEUTROPENIA; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; RADIOGRAPHY, THORACIC; ROC CURVE; SENSITIVITY AND SPECIFICITY; SERUM; TIME FACTORS;

EID: 60849094783     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01716-08     Document Type: Article

References (27)

1. Badiee, P., P. Kordbacheh, A. Alborzi, S. Malekhoseini, F. Zeini, H. Mirhendi, and M. Mahmoodi. 2007. Prospective screening in liver transplant recipients by panfungal PCR-ELISA for early diagnosis of invasive fungal infections. Liver Transpl. 13:1011-1016.
2. Boudewijns, M., P. E. Verweij, and W. J. Melchers. 2006. Molecular diagnosis of invasive aspergillosis: the long and winding road. Future Microbiol. 1:283-293.
3. Cesaro, S., C. Stenghele, E. Calore, E. Franchin, I. Cerbaro, R. Cusinato, G. Tridello, R. Manganelli, M. Carli, and G. Palu. 3 March 2008, posting date. Assessment of the lightcycler PCR assay for diagnosis of invasive aspergillosis in paediatric patients with oncohaematological diseases. Mycoses. [Epub ahead of print.]
4. Cuenca-Estrella, M., A. Gomez-Lopez, E. Mellado, M. J. Buitrago, A. Monzon, and J. L. Rodriguez-Tudela. 2006. Head-to-head comparison of the activities of currently available antifungal agents against 3,378 Spanish clinical isolates of yeasts and filamentous fungi. Antimicrob. Agents Chemother. 50:917-921.
5. De Pauw, B., T. J. Walsh, J. P. Donnelly, D. A. Stevens, J. E. Edwards, T. Calandra, P. G. Pappas, J. Maertens, O. Lortholary, C. A. Kauffman, D. W. Denning, T. F. Patterson, G. Maschmeyer, J. Bille, W. E. Dismukes, R. Herbrecht, W. W. Hope, C. C. Kibbler, B. J. Kullberg, K. A. Marr, P. Munoz, F. C. Odds, J. R. Perfect, A. Restrepo, M. Ruhnke, B. H. Segal, J. D. Sobel, T. C. Sorrell, C. Viscoli, J. R. Wingard, T. Zaoutis, and J. E. Bennett. 2008. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin. Infect. Dis. 46:1813-1821.
6. Donnelly, J. P. 2006. Polymerase chain reaction for diagnosing invasive aspergillosis: getting closer but still a ways to go. Clin. Infect. Dis. 42:487-489.
7. Einsele, H., and J. Loeffler. 2008. Contribution of new diagnostic approaches to antifungal treatment plans in high-risk haematology patients. Clin. Microbiol. Infect. 14(Suppl. 4):37-45.
8. Florent, M., S. Katsahian, A. Vekhoff, V. Levy, B. Rio, J. P. Marie, A. Bouvet, and M. Cornet. 2006. Prospective evaluation of a polymerase chain reaction-ELISA targeted to Aspergillus fumigatus and Aspergillus flavus for the early diagnosis of invasive aspergillosis in patients with hematological malignancies. J. Infect. Dis. 193:741-747.
9. Gomez-Lopez, A., M. T. Martin-Gomez, P. Martin-Davila, P. Lopez-Onrubia, J. Gavalda, J. Fortun, A. Pahissa, J. L. Rodriguez-Tudela, and M. Cuenca-Estrella. 2006. Detection of fungal DNA by real-time polymerase chain reaction: evaluation of 2 methodologies in experimental pulmonary aspergillosis. Diagn. Microbiol. Infect. Dis. 56:387-393.
10. Halliday, C., R. Hoile, T. Sorrell, G. James, S. Yadav, P. Shaw, M. Bleakley, K. Bradstock, and S. Chen. 2006. Role of prospective screening of blood for invasive aspergillosis by polymerase chain reaction in febrile neutropenic recipients of haematopoietic stem cell transplants and patients with acute leukaemia. Br. J. Haematol. 132:478-486.
11. Khot, P. D., D. L. Ko, R. C. Hackman, and D. N. Fredricks. 2008. Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. BMC Infect. Dis. 8:73.
12. Labbe, A. C., S. H. Su, M. Laverdiere, J. Pepin, C. Patino, S. Cohen, T. Kiss, S. Lachance, G. Sauvageau, L. Busque, D. C. Roy, and J. Roy. 2007. High incidence of invasive aspergillosis associated with intestinal graft-versus-host disease following nonmyeloablative transplantation. Biol. Blood Marrow Transplant. 13:1192-1200.
13. Lass-Florl, C., G. Resch, D. Nachbaur, A. Mayr, G. Gastl, J. Auberger, R. Bialek, and M. C. Freund. 2007. The value of computed tomography-guided percutaneous lung biopsy for diagnosis of invasive fungal infection in immunocompromised patients. Clin. Infect. Dis. 45:e101-e104.
14. Leswick, D. A., S. T. Webster, B. A. Wilcox, and D. A. Fladeland. 2005. Radiation cost of helical high-resolution chest CT. AJR Am. J. Roentgenol. 184:742-745.
15. Maertens, J., K. Theunissen, E. Verbeken, K. Lagrou, J. Verhaegen, M. Boogaerts, and J. V. Eldere. 2004. Prospective clinical evaluation of lower cut-offs for galactomannan detection in adult neutropenic cancer patients and haematological stem cell transplant recipients. Br. J. Haematol. 126:852-860.
16. Maertens, J., K. Theunissen, G. Verhoef, J. Verschakelen, K. Lagrou, E. Verbeken, A. Wilmer, J. Verhaegen, M. Boogaerts, and J. Van Eldere. 2005. Galactomannan and computed tomography-based preemptive antifungal therapy in neutropenic patients at high risk for invasive fungal infection: a prospective feasibility study. Clin. Infect. Dis. 41:1242-1250.
17. Marr, K. A., M. Laverdiere, A. Gugel, and W. Leisenring. 2005. Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay. Clin. Infect. Dis. 40:1762-1769.
18. Martino, R., M. Subira, M. Rovira, C. Solano, L. Vazquez, G. F. Sanz, A. Urbano-Ispizua, S. Brunet, and R. De la Camara. 2002. Invasive fungal infections after allogeneic peripheral blood stem cell transplantation: incidence and risk factors in 395 patients. Br. J. Haematol. 116:475-482.
19. Maschmeyer, G., A. Haas, and O. A. Cornely. 2007. Invasive aspergillosis: epidemiology, diagnosis and management in immunocompromised patients. Drugs 67:1567-1601.
20. Mennink-Kersten, M. A., D. Ruegebrink, N. Wasei, W. J. Melchers, and P. E. Verweij. 2006. In vitro release by Aspergillus fumigatus of galactofuranose antigens, 1,3-β-D-glucan, and DNA, surrogate markers used for diagnosis of invasive aspergillosis. J. Clin. Microbiol. 44:1711-1718.
21. Obayashi, T., K. Negishi, T. Suzuki, and N. Funata. 2008. Reappraisal of the serum (1→3)-beta-D-glucan assay for the diagnosis of invasive fungal infections - a study based on autopsy cases from 6 years. Clin. Infect. Dis. 46:1864-1870.
22. Pfeiffer, C. D., J. P. Fine, and N. Safdar. 2006. Diagnosis of invasive aspergillosis using a galactomannan assay: a meta-analysis. Clin. Infect. Dis. 42:1417-1427.
23. Rickerts, V., S. Mousset, E. Lambrecht, K. Tintelnot, R. Schwerdtfeger, E. Presterl, V. Jacobi, G. Just-Nubling, and R. Bialek. 2007. Comparison of histopathological analysis, culture, and polymerase chain reaction assays to detect invasive mold infections from biopsy specimens. Clin. Infect. Dis. 44:1078-1083.
24. Upton, A., K. A. Kirby, P. Carpenter, M. Boeckh, and K. A. Marr. 2007. Invasive aspergillosis following hematopoietic cell transplantation: outcomes and prognostic factors associated with mortality. Clin. Infect. Dis. 44:531-540.
25. White, P. L., R. Barton, M. Guiver, C. J. Linton, S. Wilson, M. Smith, B. L. Gomez, M. J. Carr, P. T. Kimmitt, S. Seaton, K. Rajakumar, T. Holyoake, C. C. Kibbler, E. Johnson, R. P. Hobson, B. Jones, and R. A. Barnes. 2006. A consensus on fungal polymerase chain reaction diagnosis? A United Kingdom-Ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections. J. Mol. Diagn. 8:376-384.
26. White, P. L., C. J. Linton, M. D. Perry, E. M. Johnson, and R. A. Barnes. 2006. The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting. Clin. Infect. Dis. 42:479-486.
27. Yoo, J. H., S. M. Choi, J. H. Choi, E. Y. Kwon, C. Park, and W. S. Shin. 2008. Construction of internal control for the quantitative assay of Aspergillus fumigatus using real-time nucleic acid sequence-based amplification. Diagn. Microbiol. Infect. Dis. 60:121-124.