Routine use of a real-time polymerase chain reaction method for detection of bloodstream infections in neutropaenic patients

Diagnostic Microbiology and Infectious Disease

Volumn 75, Issue 2, 2013, Pages 130-134

  Paolucci, Michela ^a   Stanzani, Marta ^b   Melchionda, Fraia ^a   Tolomelli, Giulia ^b   Castellani, Gastone ^a   Landini, Maria Paola ^a   Varani, Stefania ^a   Lewis, Russell E ^c   Sambri, Vittorio ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c UNIVERSITY OF HOUSTON   (United States)

Author keywords

Antibiotic therapy; Blood culture; Bloodstream infection; Haematologic cancer patient; Neutropaenia; PCR

Indexed keywords

ANTIBIOTIC AGENT; ANTIFUNGAL AGENT;

ADULT; ARTICLE; ASPERGILLOSIS; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BLOOD SAMPLING; BLOODSTREAM INFECTION; CANDIDIASIS; CHILD; CLINICAL EFFECTIVENESS; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DRUG EFFECT; EARLY DIAGNOSIS; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROCOCCAL INFECTION; ESCHERICHIA COLI; FEBRILE NEUTROPENIA; FUNGUS IDENTIFICATION; FUSARIUM; GOLD STANDARD; HUMAN; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MAJOR CLINICAL STUDY; MIXED INFECTION; NONHUMAN; PREDICTIVE VALUE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS INFECTION; STREPTOCOCCUS INFECTION;

BACTEREMIA; BACTERIA; FEVER; FUNGEMIA; FUNGI; HUMANS; MYELOPROLIFERATIVE DISORDERS; NEUTROPENIA; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84872492979     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.10.012     Document Type: Article

References (24)

1. Bloos F., Hinder F., Becker K., Sachse S., Mekontso Dessap A., Straube E., et al. A multicenter trial to compare blood culture with polymerase chain reaction in severe human sepsis. Intensive Care Med 2010, 36:241-247.
2. Bravo D., Blanquer J., Tormo M., Aguilar G., Borras R., Solano C., et al. Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients. Int J Infect Dis 2011, 15:e326-e331.
3. Bucaneve G., Micozzi A., Menichetti F., Martino P., Dionisi M.S., Martinelli G., et al. Levofloxacin to prevent bacterial infection in patients with cancer and neutropenia. N Engl J Med 2005, 353:977-987.
4. Carrigan S.D., Scott G., Tabrizian M. Toward resolving the challenges of sepsis diagnosis. Clin Chem 2004, 50:1301-1314.
5. Dorn G.L., Burson G.G., Haynes J.R. Blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol 1976, 3:258-263.
6. Hall K.K., Lyman J.A. Updated review of blood culture contamination. Clin Microbiol Rev 2006, 19:788-802.
7. Hughes W.T. Bloodstream infections in cancer patients. Eur J Cancer 2005, 41:1370-1371.
8. Klastersky J., Aoun M. Opportunistic infections in patients with cancer. Ann Oncol 2004, 15(Suppl. 4):iv329-iv335.
9. Lamoth F., Jaton K., Prod'hom G., Senn L., Bille J., Calandra T., et al. Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol 2010, 48:3510-3516.
10. Lehmann L.E., Hunfeld K.P., Emrich T., Haberhausen G., Wissing H., Hoeft A., et al. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 2008, 197:313-324.
11. Mancini N., Clerici D., Diotti R., Perotti M., Ghidoli N., De Marco D., et al. Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol 2008, 57:601-604.
12. Morrell M., Fraser V.J., Kollef M.H. Delaying the empiric treatment of candida bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality. Antimicrob Agents Chemother 2005, 49:3640-3645.
13. Nakamura A., Sugimoto Y., Ohishi K., Sugawara Y., Fujieda A., Monma F., et al. Diagnostic value of PCR analysis of bacteria and fungi from blood in empiric-therapy-resistant febrile neutropenia. J Clin Microbiol 2010, 48:2030-2036.
14. Penack O., Beinert T., Buchheidt D., Einsele H., Hebart H., Kiehl M.G., et al. Management of sepsis in neutropenia: guidelines of the infectious diseases working party (AGIHO) of the German Society of Hematology and Oncology (DGHO). Ann Hematol 2006, 85:424-433.
15. Peters R.P., van Agtmael M.A., Danner S.A., Savelkoul P.H., Vandenbroucke-Grauls C.M. New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 2004, 4:751-760.
16. Raad I., Hachem R., Hanna H., Bahna P., Chatzinikolaou I., Fang X., et al. Sources and outcome of bloodstream infections in cancer patients: the role of central venous catheters. Eur J Clin Microbiol Infect Dis 2007, 26:549-556.
17. Richardson M.D. Changing patterns and trends in systemic fungal infections. J Antimicrob Chemother 2005, 56(Suppl 1):i5-i11.
18. Tsalik E.L., Jones D., Nicholson B., Waring L., Liesenfeld O., Park L.P., et al. Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. J Clin Microbiol 2010, 48:26-33.
19. Varani S., Stanzani M., Paolucci M., Melchionda F., Castellani G., Nardi L., et al. Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR. J Infect 2009, 58:346-351.
20. von Lilienfeld-Toal M., Lehmann L.E., Raadts A.D., Hahn-Ast C., Orlopp K.S., Marklein G., et al. Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia. J Clin Microbiol 2009, 47:2405-2410.
21. Wallet F., Nseir S., Baumann L., Herwegh S., Sendid B., Boulo M., et al. Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect 2010, 16:774-779.
22. Westh H., Lisby G., Breysse F., Boddinghaus B., Chomarat M., Gant V., et al. Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 2009, 15:544-551.
23. Wilson M.L., Mitchell M., Murray P., Reiner L.G., Reller L.B., Towns S.M., et al. Principles and procedures for blood cultures: approved guidelines 2007, Clinical and Laboratory Standard Institute, Wayne, PA, USA.
24. Woo P.C., Wong S.S., Lum P.N., Hui W.T., Yuen K.Y. Cell-wall-deficient bacteria and culture-negative febrile episodes in bone-marrow-transplant recipients. Lancet 2001, 357:675-679.