Diagnostic performance of a multiple real-time PCR assay in patients with suspected sepsis hospitalized in an internal medicine ward

Journal of Clinical Microbiology

Volumn 50, Issue 4, 2012, Pages 1285-1288

  Pasqualini, Leonella ^a   Mencacci, Antonella ^a   Leli, Christian ^a   Montagna, Paolo ^a   Cardaccia, Angela ^a   Cenci, Elio ^a   Montecarlo, Ines ^a   Pirro, Matteo ^a   Di Filippo, Francesco ^a   Cistaro, Emma ^a   Schillaci, Giuseppe ^a   Bistoni, Francesco ^a   Mannarinoa, Elmo ^a  

^a UNIVERSITY OF PERUGIA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; AEROMONAS SOBRIA; AGED; ANALYTIC METHOD; ARTICLE; BLOOD CULTURE; CANDIDA ALBICANS; CLOSTRIDIUM PERFRINGENS; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; FEMALE; HAFNIA ALVEI; HOSPITALIZATION; HUMAN; INTERMETHOD COMPARISON; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; LEUCONOSTOC; LISTERIA; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; PEPTOSTREPTOCOCCUS; PRIORITY JOURNAL; PROSPECTIVE STUDY; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEPSIS; SEPTIFAST; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS; STREPTOCOCCUS DYSGALACTIAE; STREPTOCOCCUS PNEUMONIAE; SYSTEMIC INFLAMMATORY RESPONSE SYNDROME; WARD;

ADULT; AGED; AGED, 80 AND OVER; BACTEREMIA; BACTERIA; CANDIDA ALBICANS; CANDIDEMIA; DNA, RIBOSOMAL SPACER; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 84858591383     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.06793-11     Document Type: Article

References (23)

1. Adrie C, et al. 2009. Model for predicting short-term mortality of severe sepsis. Crit. Care 13:R72.
2. Bauer M, Reinhart K. 2010. Molecular diagnostics of sepsis-where are we today? Int. J. Med. Microbiol. 300:411-413.
3. Bone RC, et al. 1992. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine. Chest 101:1644-1655.
4. Casalta JP, et al. 2009. Evaluation of the LightCycler SeptiFast test in the rapid etiologic diagnostic of infectious endocarditis. Eur. J. Clin. Microbiol. Infect. Dis. 28:569-573.
5. Dierkes C, et al. 2009. Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect. Dis. 9:126.
6. Glerant JC, Hellmuth D, Schmit JL, Ducroix JP, Jounieaux V. 1999. Utility of blood cultures in community-acquired pneumonia requiring hospitalization: influence of antibiotic treatment before admission. Respir. Med. 93:208-212. (Pubitemid 29090056)
7. Jorgensen JH, et al. 1997. Controlled clinical laboratory comparison of BACTECplus aerobic/F resinmediumwith BacT/Alert aerobicFANmedium for detection of bacteremia and fungemia. J. Clin. Microbiol. 35:53-58. (Pubitemid 26422627)
8. Josefson P, et al. 2011. Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections. Eur. J. Clin. Microbiol. Infect. Dis. 30:1127-1134.
9. Lamoth F, et al. 2010. Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J. Clin. Microbiol. 48:3510-3516.
10. Lee CM, Yeh SC, Lim HK, Liu CP, Tseng HK. 2009. High prevalence rate of multidrug resistance among nosocomial pathogens in the respiratory care center of a tertiary hospital. J. Microbiol. Immunol. Infect. 42:401-404.
11. Lehmann LE, et al. 2008. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med. Microbiol. Immunol. 197:313-324.
12. Lehmann LE, et al. 2010. Improved detection of blood stream pathogens by real-time PCR in severe sepsis. Intensive Care Med. 36:49-56.
13. Leyssene D, et al. 2011. Species-driven interpretation guidelines in case of a single-sampling strategy for blood culture. Eur. J. Clin. Microbiol. Infect. Dis. 30:1537-1541.
14. Louie RF, et al. 2008. Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit. Care Med. 36:1487-1492. (Pubitemid 351620647)
15. Lucignano B, et al. 2011. Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis. J. Clin. Microbiol. 49:2252-2258.
16. Mancini N, et al. 2008. Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J. Med. Microbiol. 57:601-604. (Pubitemid 351632907)
17. McKenzie R, Reimer LG. 1987. Effect of antimicrobials on blood cultures in endocarditis. Diagn. Microbiol. Infect. Dis. 8:165-172. (Pubitemid 18059454)
18. Obara H, et al. 2011. The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in wholeblood samples. J. Infect. Chemother. 17:327-333.
19. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM. 2004. New developments in the diagnosis of bloodstream infections. Lancet Infect. Dis. 4:751-760. (Pubitemid 39574347)
20. Varani S, et al. 2009. Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR. J. Infect. 58:346-351.
21. Westh H, et al. 2009. Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin. Microbiol. Infect. 15:544-551.
22. Yanagihara K, et al. 2010. Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit. Crit. Care. 14:R159.
23. Zubert S, Funk DJ, Kumar A. 2010. Antibiotics in sepsis and septic shock: like everything else in life, timing is everything. Crit. Care Med. 38:1211-1212.