메뉴 건너뛰기




Volumn 9, Issue 6, 2014, Pages 1514-1531

Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL COUNT; CELL DIFFERENTIATION; CELL EXPANSION; CELL FUNCTION; CELL ISOLATION; CONTROLLED STUDY; EMBRYO; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VIVO STUDY; NONHUMAN; PERICYTE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; VASCULARIZATION; XENOGRAFT; ZEBRA FISH; ANIMAL; CELL PROLIFERATION; CULTURE TECHNIQUE; CYTOLOGY; PHYSIOLOGY; PROCEDURES;

EID: 84901847622     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.102     Document Type: Article
Times cited : (279)

References (56)
  • 1
    • 0032491416 scopus 로고    scopus 로고
    • Embryonic stem cell lines derived from human blastocysts
    • Thomson, J.A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147 (1998).
    • (1998) Science , vol.282 , pp. 1145-1147
    • Thomson, J.A.1
  • 2
    • 36248966518 scopus 로고    scopus 로고
    • Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    • Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (2007).
    • (2007) Cell , vol.131 , pp. 861-872
    • Takahashi, K.1
  • 3
    • 36749043230 scopus 로고    scopus 로고
    • Induced pluripotent stem cell lines derived from human somatic cells
    • Yu, J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920 (2007).
    • (2007) Science , vol.318 , pp. 1917-1920
    • Yu, J.1
  • 4
    • 62149125434 scopus 로고    scopus 로고
    • Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    • Chambers, S.M. et al. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat. Biotechnol. 27, 275-280 (2009).
    • (2009) Nat. Biotechnol. , vol.27 , pp. 275-280
    • Chambers, S.M.1
  • 5
    • 77951480489 scopus 로고    scopus 로고
    • Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development
    • Touboul, T. et al. Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology 51, 1754-1765 (2010).
    • (2010) Hepatology , vol.51 , pp. 1754-1765
    • Touboul, T.1
  • 6
    • 79952397747 scopus 로고    scopus 로고
    • Generation of anterior foregut endoderm from human embryonic and induced pluripotent stem cells
    • Green, M.D. et al. Generation of anterior foregut endoderm from human embryonic and induced pluripotent stem cells. Nat. Biotechnol. 29, 267-272 (2011).
    • (2011) Nat. Biotechnol. , vol.29 , pp. 267-272
    • Green, M.D.1
  • 7
    • 79551686425 scopus 로고    scopus 로고
    • Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
    • Spence, J.R. et al. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 470, 105-109 (2011).
    • (2011) Nature , vol.470 , pp. 105-109
    • Spence, J.R.1
  • 8
    • 82355175128 scopus 로고    scopus 로고
    • NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes
    • Elliott, D.A. et al. NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nat. Methods 8, 1037-1040 (2011).
    • (2011) Nat. Methods , vol.8 , pp. 1037-1040
    • Elliott, D.A.1
  • 9
    • 80755142726 scopus 로고    scopus 로고
    • SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells
    • Dubois, N.C. et al. SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells. Nat. Biotechnol. 29, 1011-1018 (2011).
    • (2011) Nat. Biotechnol. , vol.29 , pp. 1011-1018
    • Dubois, N.C.1
  • 10
    • 84892764101 scopus 로고    scopus 로고
    • Efficient generation of lung and airway epithelial cells from human pluripotent stem cells
    • Huang, S.X.L. et al. efficient generation of lung and airway epithelial cells from human pluripotent stem cells. Nat. Biotechnol. 1-11 (2013).
    • (2013) Nat. Biotechnol. , pp. 1-11
    • Huang, S.X.L.1
  • 11
    • 84884414984 scopus 로고    scopus 로고
    • Cerebral organoids model human brain development and microcephaly
    • Lancaster, M.A. et al. Cerebral organoids model human brain development and microcephaly. Nature 501, 373-379 (2013).
    • (2013) Nature , vol.501 , pp. 373-379
    • Lancaster, M.A.1
  • 12
    • 7244242362 scopus 로고    scopus 로고
    • Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood
    • Ingram, D.A. et al. Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood 104, 2752-2760 (2004).
    • (2004) Blood , vol.104 , pp. 2752-2760
    • Ingram, D.A.1
  • 13
    • 33847348148 scopus 로고    scopus 로고
    • Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals
    • Yoder, M.C. et al. Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals. Blood 109, 1801-1809 (2007).
    • (2007) Blood , vol.109 , pp. 1801-1809
    • Yoder, M.C.1
  • 14
    • 79961230399 scopus 로고    scopus 로고
    • Pericytes: Developmental, physiological, and pathological perspectives, problems, and promises
    • Armulik, A., Genové, G. & Betsholtz, C. Pericytes: developmental, physiological, and pathological perspectives, problems, and promises. Dev. Cell 21, 193-215 (2011).
    • (2011) Dev. Cell , vol.21 , pp. 193-215
    • Armulik, A.1    Genové, G.2    Betsholtz, C.3
  • 15
    • 80055014830 scopus 로고    scopus 로고
    • Central nervous system pericytes in health and disease
    • Winkler, E.A., Bell, R.D. & Zlokovic, B.V. Central nervous system pericytes in health and disease. Nat. Neurosci. 14, 1398-1405 (2011).
    • (2011) Nat. Neurosci. , vol.14 , pp. 1398-1405
    • Winkler, E.A.1    Bell, R.D.2    Zlokovic, B.V.3
  • 16
    • 77950538918 scopus 로고    scopus 로고
    • Thalidomide stimulates vessel maturation and reduces epistaxis in individuals with hereditary hemorrhagic telangiectasia
    • Lebrin, F. et al. Thalidomide stimulates vessel maturation and reduces epistaxis in individuals with hereditary hemorrhagic telangiectasia. Nat. Med. 16, 420-428 (2010).
    • (2010) Nat. Med. , vol.16 , pp. 420-428
    • Lebrin, F.1
  • 17
    • 84880566944 scopus 로고    scopus 로고
    • Coronary microvascular pericytes are the cellular target of sunitinib malate-induced cardiotoxicity
    • Chintalgattu, V. et al. Coronary microvascular pericytes are the cellular target of sunitinib malate-induced cardiotoxicity. Sci. Transl. Med. 5, 187ra69 (2013).
    • (2013) Sci. Transl. Med. , vol.5 , pp. 187-269
    • Chintalgattu, V.1
  • 18
    • 50849139576 scopus 로고    scopus 로고
    • A perivascular origin for mesenchymal stem cells in multiple human organs
    • Crisan, M. et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3, 301-313 (2008).
    • (2008) Cell Stem Cell , vol.3 , pp. 301-313
    • Crisan, M.1
  • 19
    • 84872088799 scopus 로고    scopus 로고
    • The meaning, the sense and the significance: Translating the science of mesenchymal stem cells into medicine
    • Bianco, P. et al. The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine. Nat. Med. 19, 35-42 (2013).
    • (2013) Nat. Med. , vol.19 , pp. 35-42
    • Bianco, P.1
  • 20
    • 84875539909 scopus 로고    scopus 로고
    • Mesenchymal stem cell: Keystone of the hematopoietic stem cell niche and a stepping-stone for regenerative medicine
    • Frenette, P.S., Pinho, S., Lucas, D. & Scheiermann, C. Mesenchymal stem cell: keystone of the hematopoietic stem cell niche and a stepping-stone for regenerative medicine. Annu. Rev. Immunol. 31, 285-316 (2013).
    • (2013) Annu. Rev. Immunol. , vol.31 , pp. 285-316
    • Frenette, P.S.1    Pinho, S.2    Lucas, D.3    Scheiermann, C.4
  • 21
    • 84891833000 scopus 로고    scopus 로고
    • Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts
    • Orlova, V.V. et al. Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts. Arterioscler. Thromb. Vasc. Biol. 34, 177-186 (2014).
    • (2014) Arterioscler. Thromb. Vasc. Biol. , vol.34 , pp. 177-186
    • Orlova, V.V.1
  • 22
    • 0037007042 scopus 로고    scopus 로고
    • Endothelial cells derived from human embryonic stem cells
    • Levenberg, S. Endothelial cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA 99, 4391-4396 (2002).
    • (2002) Proc. Natl. Acad. Sci. USA , vol.99 , pp. 4391-4396
    • Levenberg, S.1
  • 23
    • 84855407739 scopus 로고    scopus 로고
    • Multipotent vasculogenic pericytes from human pluripotent stem cells promote recovery of murine ischemic limb
    • Dar, A. et al. Multipotent vasculogenic pericytes from human pluripotent stem cells promote recovery of murine ischemic limb. Circulation 125, 87-99 (2012).
    • (2012) Circulation , vol.125 , pp. 87-99
    • Dar, A.1
  • 24
    • 65249102249 scopus 로고    scopus 로고
    • Hematopoietic and endothelial differentiation of human induced pluripotent stem cells
    • Choi, K.-D. et al. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells. Stem Cells 27, 559-567 (2009).
    • (2009) Stem Cells , vol.27 , pp. 559-567
    • Choi, K.-D.1
  • 25
    • 73849105655 scopus 로고    scopus 로고
    • VEGF induces differentiation of functional endothelium from human embryonic stem cells: Implications for tissue engineering
    • Nourse, M.B. et al. VEGF induces differentiation of functional endothelium from human embryonic stem cells: implications for tissue engineering. Arterioscler. Thromb. Vasc. Biol. 30, 80-89 (2009).
    • (2009) Arterioscler. Thromb. Vasc. Biol. , vol.30 , pp. 80-89
    • Nourse, M.B.1
  • 26
    • 84881366718 scopus 로고    scopus 로고
    • Stem cell reports
    • Adams, W.J. et al. Stem cell reports. Stem Cell Rep. 1, 105-113 (2013).
    • (2013) Stem Cell Rep. , vol.1 , pp. 105-113
    • Adams, W.J.1
  • 27
    • 78649647544 scopus 로고    scopus 로고
    • A mesoderm-derived precursor for mesenchymal stem and endothelial cells
    • Vodyanik, M.A. et al. A mesoderm-derived precursor for mesenchymal stem and endothelial cells. Cell Stem Cell 7, 718-729 (2010).
    • (2010) Cell Stem Cell , vol.7 , pp. 718-729
    • Vodyanik, M.A.1
  • 28
    • 84881118373 scopus 로고    scopus 로고
    • Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
    • Kusuma, S. et al. Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix. Proc. Natl. Acad. Sci. USA 110, 12601-12606 (2013).
    • (2013) Proc. Natl. Acad. Sci. USA , vol.110 , pp. 12601-12606
    • Kusuma, S.1
  • 29
    • 76349087336 scopus 로고    scopus 로고
    • Expansion and maintenance of human embryonic stem cell-derived endothelial cells by TGFβ inhibition is Id1 dependent
    • James, D. et al. Expansion and maintenance of human embryonic stem cell-derived endothelial cells by TGFβ inhibition is Id1 dependent. Nat. Biotechnol. 28, 161-166 (2010).
    • (2010) Nat. Biotechnol. , vol.28 , pp. 161-166
    • James, D.1
  • 30
    • 84881114127 scopus 로고    scopus 로고
    • Generation of functionally competent and durable engineered blood vessels from human induced pluripotent stem cells
    • Samuel, R. et al. Generation of functionally competent and durable engineered blood vessels from human induced pluripotent stem cells. Proc. Natl. Acad. Sci. USA 110, 12774-12779 (2013).
    • (2013) Proc. Natl. Acad. Sci. USA , vol.110 , pp. 12774-12779
    • Samuel, R.1
  • 31
    • 84893704122 scopus 로고    scopus 로고
    • Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature
    • Park, T.S. et al. Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature. Circulation 129, 359-372 (2014).
    • (2014) Circulation , vol.129 , pp. 359-372
    • Park, T.S.1
  • 32
    • 84871558573 scopus 로고    scopus 로고
    • Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells
    • White, M.P. et al. Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells. Stem Cells 31, 92-103 (2012).
    • (2012) Stem Cells , vol.31 , pp. 92-103
    • White, M.P.1
  • 33
    • 84869131455 scopus 로고    scopus 로고
    • Derivation of endothelial cells from human embryonic stem cells in fully defined medium enables identification of lysophosphatidic acid and platelet activating factor as regulators of eNOS localization
    • Costa, M. et al. Derivation of endothelial cells from human embryonic stem cells in fully defined medium enables identification of lysophosphatidic acid and platelet activating factor as regulators of eNOS localization. Stem Cell Res. 10, 103-117 (2013).
    • (2013) Stem Cell Res. , vol.10 , pp. 103-117
    • Costa, M.1
  • 34
    • 84864874302 scopus 로고    scopus 로고
    • Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells
    • Lippmann, E.S. et al. Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells. Nat. Biotechnol. 30, 783-791 (2012).
    • (2012) Nat. Biotechnol. , vol.30 , pp. 783-791
    • Lippmann, E.S.1
  • 35
    • 84856748072 scopus 로고    scopus 로고
    • Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility
    • Cheung, C., Bernardo, A.S., Trotter, M.W.B., Pedersen, R.A. & Sinha, S. Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility. Nat. Biotechnol. 30, 165-173 (2012).
    • (2012) Nat. Biotechnol. , vol.30 , pp. 165-173
    • Cheung, C.1    Bernardo, A.S.2    Trotter, M.W.B.3    Pedersen, R.A.4    Sinha, S.5
  • 36
    • 43149101280 scopus 로고    scopus 로고
    • A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies
    • Ng, E.S., Davis, R., Stanley, E.G. & Elefanty, A.G. A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies. Nat. Protoc. 3, 768-776 (2008).
    • (2008) Nat. Protoc. , vol.3 , pp. 768-776
    • Ng, E.S.1    Davis, R.2    Stanley, E.G.3    Elefanty, A.G.4
  • 37
    • 84863560929 scopus 로고    scopus 로고
    • Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling
    • Lian, X. et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc. Natl. Acad. Sci. USA 109, E1848-E1857 (2012).
    • (2012) Proc. Natl. Acad. Sci. USA , vol.109
    • Lian, X.1
  • 38
    • 74049146291 scopus 로고    scopus 로고
    • A novel imaging-based high-throughput screening approach to anti-angiogenic drug discovery
    • Evensen, L., Micklem, D.R., Link, W. & Lorens, J.B. A novel imaging-based high-throughput screening approach to anti-angiogenic drug discovery. Cytometry A 77, 41-51 (2010).
    • (2010) Cytometry A , vol.77 , pp. 41-51
    • Evensen, L.1    Micklem, D.R.2    Link, W.3    Lorens, J.B.4
  • 39
    • 84934440196 scopus 로고    scopus 로고
    • Image-based high-throughput screening for inhibitors of angiogenesis
    • Evensen, L., Link, W. & Lorens, J.B. Image-based high-throughput screening for inhibitors of angiogenesis. Methods Mol. Biol. 931, 139-151 (2013).
    • (2013) Methods Mol. Biol. , vol.931 , pp. 139-151
    • Evensen, L.1    Link, W.2    Lorens, J.B.3
  • 40
    • 67249129599 scopus 로고    scopus 로고
    • Mural cell associated VEGF is required for organotypic vessel formation
    • Evensen, L. et al. Mural cell associated VEGF is required for organotypic vessel formation. PLoS ONE 4, e5798 (2009).
    • (2009) PLoS ONE , vol.4
    • Evensen, L.1
  • 41
    • 81155134376 scopus 로고    scopus 로고
    • A computational tool for quantitative analysis of vascular networks
    • Zudaire, E., Gambardella, L., Kurcz, C. & Vermeren, S. A computational tool for quantitative analysis of vascular networks. PLoS ONE 6, e27385 (2011).
    • (2011) PLoS ONE , vol.6
    • Zudaire, E.1    Gambardella, L.2    Kurcz, C.3    Vermeren, S.4
  • 42
    • 58149510531 scopus 로고    scopus 로고
    • CellProfiler Analyst: Data exploration and analysis software for complex image-based screens
    • Jones, T.R. et al. CellProfiler Analyst: data exploration and analysis software for complex image-based screens. BMC Bioinformatics 9, 482 (2008).
    • (2008) BMC Bioinformatics , vol.9 , pp. 482
    • Jones, T.R.1
  • 43
    • 48649088341 scopus 로고    scopus 로고
    • Catch of the day: Zebrafish as a human cancer model
    • Stoletov, K. & Klemke, R. Catch of the day: zebrafish as a human cancer model. Oncogene 27, 4509-4520 (2008).
    • (2008) Oncogene , vol.27 , pp. 4509-4520
    • Stoletov, K.1    Klemke, R.2
  • 44
    • 34347211064 scopus 로고    scopus 로고
    • New waves of discovery: Modeling cancer in zebrafish
    • Goessling, W., North, T.E. & Zon, L.I. New waves of discovery: modeling cancer in zebrafish. J. Clin. Oncol. 25, 2473-2479 (2007).
    • (2007) J. Clin. Oncol. , vol.25 , pp. 2473-2479
    • Goessling, W.1    North, T.E.2    Zon, L.I.3
  • 45
    • 12144273121 scopus 로고    scopus 로고
    • In vivo drug discovery in the zebrafish
    • Zon, L.I. & Peterson, R.T. In vivo drug discovery in the zebrafish. Nat. Rev. Drug Discov. 4, 35-44 (2005).
    • (2005) Nat. Rev. Drug Discov. , vol.4 , pp. 35-44
    • Zon, L.I.1    Peterson, R.T.2
  • 47
    • 84878183437 scopus 로고    scopus 로고
    • Snail and Slug, key regulators of TGF-β-induced EMT, are sufficient for the induction of single-cell invasion
    • Naber, H.P.H., Drabsch, Y., Snaar-Jagalska, B.E., ten Dijke, P. & van Laar, T. Snail and Slug, key regulators of TGF-β-induced EMT, are sufficient for the induction of single-cell invasion. Biochem. Biophys. Res. Commun. 435, 58-63 (2013).
    • (2013) Biochem. Biophys. Res. Commun. , vol.435 , pp. 58-63
    • Naber, H.P.H.1    Drabsch, Y.2    Snaar-Jagalska, B.E.3    Ten Dijke, P.4    Van Laar, T.5
  • 48
    • 84883827391 scopus 로고    scopus 로고
    • TRAF4 promotes TGF-β receptor signaling and drives breast cancer metastasis
    • Zhang, L. et al. TRAF4 promotes TGF-β receptor signaling and drives breast cancer metastasis. Mol. Cell 51, 559-572 (2013).
    • (2013) Mol. Cell , vol.51 , pp. 559-572
    • Zhang, L.1
  • 49
    • 84863218422 scopus 로고    scopus 로고
    • USP4 is regulated by AKT phosphorylation and directly deubiquitylates TGF-β type i receptor
    • Zhang, L. et al. USP4 is regulated by AKT phosphorylation and directly deubiquitylates TGF-β type I receptor. Nat. Cell Biol. 14, 717-726 (2012).
    • (2012) Nat. Cell Biol. , vol.14 , pp. 717-726
    • Zhang, L.1
  • 50
    • 84863725047 scopus 로고    scopus 로고
    • Neutrophil-mediated experimental metastasis is enhanced by VEGFR inhibition in a zebrafish xenograft model
    • He, S. et al. Neutrophil-mediated experimental metastasis is enhanced by VEGFR inhibition in a zebrafish xenograft model. J. Pathol. 227, 431-445 (2012).
    • (2012) J. Pathol. , vol.227 , pp. 431-445
    • He, S.1
  • 51
    • 84856726745 scopus 로고    scopus 로고
    • Automated whole-animal bio-imaging assay for human cancer dissemination
    • Ghotra, V.P.S. et al. Automated whole-animal bio-imaging assay for human cancer dissemination. PLoS ONE 7, e31281 (2012).
    • (2012) PLoS ONE , vol.7
    • Ghotra, V.P.S.1
  • 52
    • 28844467657 scopus 로고    scopus 로고
    • Human embryonic stem cells: Towards therapies for cardiac disease. Derivation of a Dutch human embryonic stem cell line
    • Online
    • van de Stolpe, A. et al. Human embryonic stem cells: towards therapies for cardiac disease. Derivation of a Dutch human embryonic stem cell line. Reprod. BioMed. Online 11, 476-485 (2005).
    • (2005) Reprod. BioMed , vol.11 , pp. 476-485
    • Van De Stolpe, A.1
  • 53
    • 84877330336 scopus 로고    scopus 로고
    • Polycistronic lentivirus induced pluripotent stem cells from skin biopsies after long term storage, blood outgrowth endothelial cells and cells from milk teeth
    • Dambrot, C. et al. Polycistronic lentivirus induced pluripotent stem cells from skin biopsies after long term storage, blood outgrowth endothelial cells and cells from milk teeth. Differentiation 85, 101-109 (2013).
    • (2013) Differentiation , vol.85 , pp. 101-109
    • Dambrot, C.1
  • 54
    • 52749085345 scopus 로고    scopus 로고
    • A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis
    • Davis, R.P. et al. A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis. Nat. Protoc. 3, 1550-1558 (2008).
    • (2008) Nat. Protoc. , vol.3 , pp. 1550-1558
    • Davis, R.P.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.