Image-based high-throughput screening for inhibitors of angiogenesis

Methods in Molecular Biology

Volumn 931, Issue , 2012, Pages 139-151

  Evensen, Lasse ^a   Link, Wolfgang ^b   Lorens, James B ^a  

^a UNIVERSITY OF BERGEN   (Norway)

^b UNIVERSITY OF ALGARVE   (Portugal)

Author keywords

Angiogenesis; Automated fluorescence microscopy; Cell sorting; Co culture; Fluorescent protein; High content screening; High throughput screening; Image analysis; Retroviral vector transduction

Indexed keywords

ANGIOGENESIS INHIBITOR; RETROVIRUS VECTOR;

ANGIOGENESIS; ANTIANGIOGENIC ACTIVITY; ARTICLE; COCULTURE; DRUG RESEARCH; FLUORESCENCE MICROSCOPY; HIGH THROUGHPUT SCREENING; HUMAN; IMAGE ANALYSIS; PRIORITY JOURNAL;

EID: 84934440196     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-56-4_8     Document Type: Article

References (7)

1. Evensen L et al (2009) Mural cell associated VEGF is required for organotypic vessel formation. PLoS One 4:e5798
2. Zanella F, Lorens JB, Link W (2010) High content screening: seeing is believing. Trends Biotechnol 28:237-245
3. Swift S et al (2001) Rapid production of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc Immunol Chapter 10:Unit 10.17C
4. Wood JM et al (2000) PTK787/ZK 222584, a novel and potent inhibitor of vascular endothelial growth factor receptor tyrosine kinases, impairs vascular endothelial growth factor-induced responses and tumor growth after oral administration. Cancer Res 60:2178-2189
5. Lee SH et al (2005) In vivo target modulation and biological activity of CHIR-258, a multitargeted growth factor receptor kinase inhibitor, in colon cancer models. Clin Cancer Res 11:3633-3641
6. Lorens JB et al (2000) Retroviral delivery of peptide modulators of cellular functions. Mol Ther 1:438-447
7. Shaner NC et al (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22:1567-1572