Evaluation of high-capacity cation exchange chromatography for direct capture of monoclonal antibodies from high-titer cell culture processes

Biotechnology and Bioengineering

Volumn 111, Issue 7, 2014, Pages 1354-1364

  Tao, Yinying ^a   Ibraheem, Aladein ^a   Conley, Lynn ^a   Cecchini, Douglas ^a   Ghose, Sanchayita ^a  

^a BIOGEN   (United States)

Author keywords

Cation exchange chromatography; Direct capture; High capacity; Host cell proteins; Monoclonal antibody

Indexed keywords

CELL CULTURE; CHROMATOGRAPHY; ION EXCHANGERS; MOLECULAR BIOLOGY; MONOCLONAL ANTIBODIES; NEUTRON ABSORPTION; POSITIVE IONS; PROTEINS; QUALITY CONTROL; RESINS; SURFACE PLASMON RESONANCE;

ANTIBODY PURIFICATION; CATION EXCHANGE CHROMATOGRAPHY; CATIONEXCHANGE CHROMATOGRAPHY (CEX); CELL CULTURE PROCESS; DIRECT CAPTURE; HIGH CAPACITY; HOST CELL PROTEIN; PROCESS PERFORMANCE;

LOADING;

CATION EXCHANGE RESIN; MONOCLONAL ANTIBODY; PROTEIN A; RESIN;

ANTIBODY DETECTION; ANTIBODY TITER; ARTICLE; CATION EXCHANGE; CELL CULTURE; CONDUCTANCE; ELUTION; ENVIRONMENTAL TEMPERATURE; HIGH TITER CELL CULTURE; ION EXCHANGE CHROMATOGRAPHY; PARTICLE SIZE; PH; PROCESS OPTIMIZATION; BIOTECHNOLOGY; COST; CULTURE TECHNIQUE; ECONOMICS; ISOLATION AND PURIFICATION; PHARMACEUTICS; PROCEDURES;

ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CHROMATOGRAPHY, ION EXCHANGE; COSTS AND COST ANALYSIS; TECHNOLOGY, PHARMACEUTICAL;

EID: 84901640129     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25192     Document Type: Article

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