Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 848, Issue 1, 2007, Pages 151-158

  Stein, Andreas ^a   Kiesewetter, André ^a  

^a MERCK KGAA   (Germany)

Author keywords

Cation exchange chromatography; Comparative study; Host cell protein removal; Monoclonal antibodies; Purification; Residence time; Resin screening

Indexed keywords

BIOLEACHING; CHROMATOGRAPHY; ION EXCHANGE; MONOCLONAL ANTIBODIES; PH EFFECTS; PURIFICATION;

BINDING CAPACITIES; HOST CELL PROTEIN REMOVAL; RESIN SCREENING;

POSITIVE IONS;

CELL PROTEIN; LIGAND; MONOCLONAL ANTIBODY; PROTEIN A;

ARTICLE; BINDING AFFINITY; DRUG PURIFICATION; ELUTION; HOST CELL; ION EXCHANGE; ION EXCHANGE CHROMATOGRAPHY; PH MEASUREMENT; PRIORITY JOURNAL; RELIABILITY; SCREENING; TITRIMETRY;

ANIMALS; ANTIBODIES, MONOCLONAL; CATION EXCHANGE RESINS; CATIONS; CELL LINE, TUMOR; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HYDROGEN-ION CONCENTRATION; PROTEIN BINDING; REPRODUCIBILITY OF RESULTS;

EID: 33847690744     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2006.10.010     Document Type: Article

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