Antibody variable region interactions with protein A: Implications for the development of generic purification processes

Biotechnology and Bioengineering

Volumn 92, Issue 6, 2005, Pages 665-673

  Ghose, Sanchayita ^a   Allen, Martin ^a   Hubbard, Brian ^a   Brooks, Clayton ^a   Cramer, Steven M ^b  

^a AMGEN INC   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Antibodies; Elution pH; Protein A chromatography; Variable region interactions

Indexed keywords

ANTIBODIES; CHROMATOGRAPHIC ANALYSIS; MOLECULAR STRUCTURE; PH EFFECTS; PURIFICATION;

ELUTION PH; PROTEIN A CHROMATOGRAPHY; VARIABLE REGION INTERACTION;

PROTEINS;

ANTIBODY; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; PROTEIN A; RESIN; STAPHYLOCOCCUS PROTEIN A;

ANTIBODY AFFINITY; ANTIBODY COMBINING SITE; ANTIBODY SPECIFICITY; ARTICLE; CHROMATOGRAPHY; ELUTION; HYDROGEN BOND; HYDROPHOBICITY; IMMUNOGLOBULIN CONSTANT REGION; IMMUNOGLOBULIN VARIABLE REGION; ISOTHERMAL TITRATION CALORIMETRY; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS AUREUS; TURBIDITY; X RAY CRYSTALLOGRAPHY;

ANIMALS; ANTIBODIES, MONOCLONAL; BINDING SITES, ANTIBODY; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN VARIABLE REGION; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCAL PROTEIN A;

STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 28844477910     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20729     Document Type: Article

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