Binding capacity differences for antibodies and Fc-fusion proteins on protein A chromatographic materials

Biotechnology and Bioengineering

Volumn 96, Issue 4, 2007, Pages 768-779

  Ghose, Sanchayita ^a   Hubbard, Brian ^a   Cramer, Steven M ^b  

^a AMGEN INC   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Binding capacity; Fc fusion proteins; Monoclonal antibodies; Protein A chromatography; Steric hindrance

Indexed keywords

BIOMOLECULES; CHROMATOGRAPHIC ANALYSIS; ENZYME IMMOBILIZATION; MASS TRANSFER; MONOCLONAL ANTIBODIES; PURIFICATION;

BINDING CAPACITY; CHROMATOGRAPHIC MATERIALS; FC-FUSION PROTEINS; PROTEIN A CHROMATOGRAPHY; STERIC HINDRANCE;

PROTEINS;

ANTIBODY; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; PROTEIN A;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CHO CELL; CHROMATOGRAPHY; CONTROLLED STUDY; DIFFUSION COEFFICIENT; NONHUMAN; STOICHIOMETRY;

ANTIBODY AFFINITY; CHROMATOGRAPHY, AFFINITY; IMMUNOGLOBULIN FC FRAGMENTS; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCAL PROTEIN A; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 33947138076     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21044     Document Type: Article

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