Capture of human monoclonal antibodies from cell culture supernatant by ion exchange media exhibiting high charge density

Biotechnology and Bioengineering

Volumn 60, Issue 6, 1998, Pages 689-698

  Necina, Roman ^a   Amatschek, Karin ^a   Jungbauer, A ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Cation exchange chromatography; Cell culture; Cell culture medium; Purification; Serum free; Therapeutic antibodies

Indexed keywords

CELL CULTURE; CHROMATOGRAPHY; GENETIC ENGINEERING; GROWTH KINETICS; ION EXCHANGERS; IONIC STRENGTH; PH EFFECTS; PROTEINS; PURIFICATION;

CELL CULTURE SUPERNATANT; HUMAN MONOCLONAL ANTIBODIES; ION EXCHANGE MEDIUM; SERUM FREE; THERAPEUTIC ANTIBODIES;

MONOCLONAL ANTIBODIES;

MONOCLONAL ANTIBODY; SEPHAROSE; SILICON DIOXIDE;

ANTIBODY PRODUCTION; ARTICLE; CATION EXCHANGE; CELL CULTURE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FETAL CALF SERUM; FLOW RATE; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; HYBRIDOMA; HYDROGEL; IONIC STRENGTH; ISOELECTRIC POINT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION;

ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, GEL; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; HYBRIDOMAS; HYDROGEN-ION CONCENTRATION; ION EXCHANGE RESINS;

EID: 0032553850     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19981220)60:6<689::AID-BIT6>3.0.CO;2-M     Document Type: Article

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