Stable 293 T and CHO cell lines expressing cleaved, stable HIV-1 envelope glycoprotein trimers for structural and vaccine studies

Retrovirology

Volumn 11, Issue 1, 2014, Pages

  Chung, Nancy P Y ^a   Matthews, Katie ^a,d   Kim, Helen J ^b   Ketas, Thomas J ^a   Golabek, Michael ^a   de los Reyes, Kevin ^a   Korzun, Jacob ^a   Yasmeen, Anila ^a   Sanders, Rogier W ^a,c   Klasse, Per J ^a   Wilson, Ian A ^b   Ward, Andrew B ^b   Marozsan, Andre J ^a,e   Moore, John P ^a   Cupo, Albert ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

^d ALEXION PHARMACEUTICALS   (United States)

^e Innovimmune Biotherapeutics Inc   (United States)

Author keywords

BG505 SOSIP.664 gp140 trimers; Flp In cells; HIV 1 envelope glycoproteins; Neutralizing antibodies; Vaccination

Indexed keywords

EPITOPE; GLYCOPROTEIN GP 140; NEUTRALIZING ANTIBODY; VIRUS ENVELOPE PROTEIN; BG505 SOSIP.664 GP140 PROTEIN TRIMER; FURIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; GLYCOPROTEIN; GLYCOPROTEIN GP 120; GLYCOPROTEIN GP 41; GP140 ENVELOPE PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; VACCINE; VIRUS ANTIGEN;

ANIMAL CELL; ANTIGEN PRESENTATION; ANTIGENICITY; ARTICLE; CHO CELL LINE; CONTROLLED STUDY; EXPRESSION VECTOR; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; T LYMPHOCYTE; 293 T CELL LINE; CELL CULTURE; CELL LINE; DIFFERENTIAL SCANNING CALORIMETRY; DOT HYBRIDIZATION; ELECTRON MICROSCOPY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GOOD LABORATORY PRACTICE; HAMSTER; HUMAN; HUMAN CELL; IMMUNOGENICITY; PROTEIN SECRETION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; SURFACE PLASMON RESONANCE; TRANSIENT TRANSFECTION; WESTERN BLOTTING; ANIMAL; BIOSYNTHESIS; CRICETULUS; GENE EXPRESSION; GENETICS; HEK293 CELL LINE; IMMUNOLOGY; METABOLISM; PROTEIN MULTIMERIZATION;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS 1; MAMMALIA;

ANIMALS; ANTIBODIES, NEUTRALIZING; ANTIGENS, VIRAL; CELL LINE; CHO CELLS; CRICETULUS; ENV GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; FURIN; GENE EXPRESSION; GLYCOPROTEINS; HEK293 CELLS; HIV ANTIBODIES; HIV ENVELOPE PROTEIN GP120; HIV ENVELOPE PROTEIN GP41; HIV-1; HUMANS; PROTEIN MULTIMERIZATION; RECOMBINANT PROTEINS; VACCINES;

EID: 84901499773     PISSN: None     EISSN: 17424690     Source Type: Journal    
DOI: 10.1186/1742-4690-11-33     Document Type: Article

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