A silencer-proximal intronic region is required for sustained CD4 expression in postselection thymocytes

Journal of Immunology

Volumn 192, Issue 10, 2014, Pages 4620-4627

  Henson, David M ^a   Chou, Chun ^a   Sakurai, Nagisa ^a   Egawa, Takeshi ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; DEOXYRIBONUCLEASE I;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CD8+ T LYMPHOCYTE; CELL DIFFERENTIATION; CELL LINEAGE; CELL MATURATION; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; CPG ISLAND; DNA METHYLATION; EPIGENETICS; INTRON; MOLECULAR DOCKING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; T LYMPHOCYTE; THYMOCYTE; UPREGULATION;

ANIMALS; ANTIGENS, CD4; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; DNA METHYLATION; GENE EXPRESSION REGULATION; GENE SILENCING; INTRONS; MICE; MICE, KNOCKOUT; SILENCER ELEMENTS, TRANSCRIPTIONAL; THYMOCYTES;

EID: 84901276890     PISSN: 00221767     EISSN: 15506606     Source Type: Journal    
DOI: 10.4049/jimmunol.1302374     Document Type: Article

References (32)

1. Kappes, D. J., X. He, and X. He. 2005. CD4-CD8 lineage commitment: an inside view. Nat. Immunol. 6: 761-766.
2. Singer, A., S. Adoro, and J. H. Park. 2008. Lineage fate and intense debate: myths, models and mechanisms of CD4- versus CD8-lineage choice. Nat. Rev. Immunol. 8: 788-801.
3. Collins, A., D. R. Littman, and I. Taniuchi. 2009. RUNX proteins in transcription factor networks that regulate T-cell lineage choice. Nat. Rev. Immunol. 9: 106-115.
4. Brugnera, E., A. Bhandoola, R. Cibotti, Q. Yu, T. I. Guinter, Y. Yamashita, S. O. Sharrow, and A. Singer. 2000. Coreceptor reversal in the thymus: signaled CD4+8+ thymocytes initially terminate CD8 transcription even when differentiating into CD8+ T cells. Immunity 13: 59-71.
5. Kioussis, D., and W. Ellmeier. 2002. Chromatin and CD4, CD8A and CD8B gene expression during thymic differentiation. Nat. Rev. Immunol. 2: 909-919.
6. Adoro, S., T. McCaughtry, B. Erman, A. Alag, F. Van Laethem, J. H. Park, X. Tai, M. Kimura, L. Wang, A. Grinberg, et al. 2012. Coreceptor gene imprinting governs thymocyte lineage fate. EMBO J. 31: 366-377.
7. Sarafova, S. D., B. Erman, Q. Yu, F. Van Laethem, T. Guinter, S. O. Sharrow, L. Feigenbaum, K. F. Wildt, W. Ellmeier, and A. Singer. 2005. Modulation of coreceptor transcription during positive selection dictates lineage fate independently of TCR/coreceptor specificity. Immunity 23: 75-87.
8. Chong, M. M., N. Simpson, M. Ciofani, G. Chen, A. Collins, and D. R. Littman. 2010. Epigenetic propagation of CD4 expression is established by the Cd4 proximal enhancer in helper T cells. Genes Dev. 24: 659-669.
9. Taniuchi, I., W. Ellmeier, and D. R. Littman. 2004. The CD4/CD8 lineage choice: new insights into epigenetic regulation during T cell development. Adv. Immunol. 83: 55-89.
10. Sawada, S., J. D. Scarborough, N. Killeen, and D. R. Littman. 1994. A lineage-specific transcriptional silencer regulates CD4 gene expression during T lymphocyte development. Cell 77: 917-929.
11. Taniuchi, I., M. J. Sunshine, R. Festenstein, and D. R. Littman. 2002. Evidence for distinct CD4 silencer functions at different stages of thymocyte differentiation. Mol. Cell 10: 1083-1096.
12. Manjunath, N., P. Shankar, B. Stockton, P. D. Dubey, J. Lieberman, and U. H. von Andrian. 1999. A transgenic mouse model to analyze CD8(+) effector T cell differentiation in vivo. Proc. Natl. Acad. Sci. USA 96: 13932-13937.
13. Zou, Y. R., M. J. Sunshine, I. Taniuchi, F. Hatam, N. Killeen, and D. R. Littman. 2001. Epigenetic silencing of CD4 in T cells committed to the cytotoxic lineage. Nat. Genet. 29: 332-336.
14. Lee, P. P., D. R. Fitzpatrick, C. Beard, H. K. Jessup, S. Lehar, K. W. Makar, M. Pérez-Melgosa, M. T. Sweetser, M. S. Schlissel, S. Nguyen, et al. 2001. A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival. Immunity 15: 763-774.
15. de Boer, J., A. Williams, G. Skavdis, N. Harker, M. Coles, M. Tolaini, T. Norton, K. Williams, K. Roderick, A. J. Potocnik, and D. Kioussis. 2003. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur. J. Immunol. 33: 314-325.
16. Koller, B. H., P. Marrack, J. W. Kappler, and O. Smithies. 1990. Normal development of mice deficient in beta 2M, MHC class I proteins, and CD8+ T cells. Science 248: 1227-1230.
17. Nakayamada, S., Y. Kanno, H. Takahashi, D. Jankovic, K. T. Lu, T. A. Johnson, H. W. Sun, G. Vahedi, O. Hakim, R. Handon, et al. 2011. Early Th1 cell differentiation is marked by a Tfh cell-like transition. Immunity 35: 919-931.
18. Wei, G., B. J. Abraham, R. Yagi, R. Jothi, K. Cui, S. Sharma, L. Narlikar, D. L. Northrup, Q. Tang, W. E. Paul, et al. 2011. Genome-wide analyses of transcription factor GATA3-mediated gene regulation in distinct T cell types. Immunity 35: 299-311.
19. Langmead, B., and S. L. Salzberg. 2012. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9: 357-359.
20. Ciofani, M., A. Madar, C. Galan, M. Sellars, K. Mace, F. Pauli, A. Agarwal, W. Huang, C. N. Parkurst, M. Muratet, et al. 2012. A validated regulatory network for Th17 cell specification. Cell 151: 289-303.
21. Egawa, T., and D. R. Littman. 2011. Transcription factor AP4 modulates reversible and epigenetic silencing of the Cd4 gene. Proc. Natl. Acad. Sci. USA 108: 14873-14878.
22. Egawa, T., and D. R. Littman. 2008. ThPOK acts late in specification of the helper T cell lineage and suppresses Runx-mediated commitment to the cytotoxic T cell lineage. Nat. Immunol. 9: 1131-1139.
23. He, X., X. He, V. P. Dave, Y. Zhang, X. Hua, E. Nicolas, W. Xu, B. A. Roe, and D. J. Kappes. 2005. The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment. Nature 433: 826-833.
24. Muroi, S., Y. Naoe, C. Miyamoto, K. Akiyama, T. Ikawa, K. Masuda, H. Kawamoto, and I. Taniuchi. 2008. Cascading suppression of transcriptional silencers by ThPOK seals helper T cell fate. Nat. Immunol. 9: 1113-1121.
25. Wang, L., K. F. Wildt, E. Castro, Y. Xiong, L. Feigenbaum, L. Tessarollo, and R. Bosselut. 2008. The zinc finger transcription factor Zbtb7b represses CD8- lineage gene expression in peripheral CD4+ T cells. Immunity 29: 876-887.
26. Bird, A. P., and A. P. Wolffe. 1999. Methylation-induced repression-belts, braces, and chromatin. Cell 99: 451-454.
27. Collins, A., S. L. Hewitt, J. Chaumeil, M. Sellars, M. Micsinai, J. Allinne, F. Parisi, E. P. Nora, D. J. Bolland, A. E. Corcoran, et al. 2011. RUNX transcription factor-mediated association of Cd4 and Cd8 enables coordinate gene regulation. Immunity 34: 303-314.
28. Kappes, D. J. 2010. Expanding roles for ThPOK in thymic development. Immunol. Rev. 238: 182-194.
29. Taniuchi, I., and W. Ellmeier. 2011. Transcriptional and epigenetic regulation of CD4/CD8 lineage choice. Adv. Immunol. 110: 71-110.
30. Xiong, Y., and R. Bosselut. 2011. The enigma of CD4-lineage specification. Eur. J. Immunol. 41: 568-574.
31. Stritesky, G. L., Y. Xing, J. R. Erickson, L. A. Kalekar, X. Wang, D. L. Mueller, S. C. Jameson, and K. A. Hogquist. 2013. Murine thymic selection quantified using a unique method to capture deleted T cells. Proc. Natl. Acad. Sci. USA 110: 4679-4684.
32. Leung, R. K., K. Thomson, A. Gallimore, E. Jones, M. Van den Broek, S. Sierro, A. R. Alsheikhly, A. McMichael, and A. Rahemtulla. 2001. Deletion of the CD4 silencer element supports a stochastic mechanism of thymocyte lineage commitment. Nat. Immunol. 2: 1167-1173.