Small-angle X-ray scattering screening complements conventional biophysical analysis: Comparative structural and biophysical analysis of monoclonal antibodies IgG1, IgG2, and IgG4

Journal of Pharmaceutical Sciences

Volumn 103, Issue 6, 2014, Pages 1701-1710

  Tian, Xinsheng ^a   Langkilde, Annette E ^a   Thorolfsson, Matthias ^b   Rasmussen, Hanne B ^b   Vestergaard, Bente ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b NOVO NORDISK A S   (Denmark)

Author keywords

analysis; IgG antibody; physicochemical properties; protein aggregation; protein formulation; small angle X ray scattering (SAXS); stability

Indexed keywords

ACETIC ACID; CITRATE SODIUM; GLYCINE; GLYCYLGLYCINE; HISTIDINE; IMIDAZOLE; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN G2A ANTIBODY; IMMUNOGLOBULIN G4; POLYSORBATE 80; SUCCINIC ACID; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY;

ARTICLE; CAPILLARY ISOELECTRIC FOCUSING; COMPARATIVE STUDY; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HUMAN; INFRARED SPECTROSCOPY; OLIGOMERIZATION; PROTEIN ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY; BIOPHYSICS; CHEMISTRY; CLASSIFICATION; GEL CHROMATOGRAPHY; PROTEIN CONFORMATION; SMALL ANGLE SCATTERING;

ANTIBODIES, MONOCLONAL; BIOPHYSICS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; IMMUNOGLOBULIN G; PROTEIN CONFORMATION; SCATTERING, SMALL ANGLE;

EID: 84901276478     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.23964     Document Type: Article

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