Metabolic profiling reveals therapeutic biomarkers of processed Aconitum Carmichaeli Debx in treating hydrocortisone induced Kidney-Yang deficiency syndrome rats

Journal of Ethnopharmacology

Volumn 152, Issue 3, 2014, Pages 585-593

  Tan, Yong ^a   Liu, Xinru ^b   Lu, Cheng ^a,c   He, Xiaojuan ^a   Li, Jian ^d   Xiao, Cheng ^e   Jiang, Miao ^a   Yang, Jing ^a   Zhou, Ke ^a,f   Zhang, Zhongxiao ^b   Zhang, Weidong ^b   Lu, Aiping ^a,c  

^a INSTITUTE OF CHINESE MATERIA MEDICA   (China)

^b SECOND MILITARY MEDICAL UNIVERSITY   (China)

^c HONG KONG BAPTIST UNIVERSITY   (Hong Kong)

^d BEIJING UNIVERSITY OF CHINESE MEDICINE   (China)

^e CHINA JAPAN FRIENDSHIP HOSPITAL   (China)

^f HUNAN UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Kidney Yang deficiency syndrome rats; Metabolic profiling; Processed Aconitum carmichaeli debx; Therapeutic biomarkers

Indexed keywords

(4 HYDROXYPHENYL)ACETALDEHYDE; 5 HYDROXYINDOL 3 ACETIC ACID; 5' PHOSPHORIBOSYL N FORMYLGLYCINAMIDE; ACONITUM CARMICHAELII DEBX EXTRACT; ACONITUM EXTRACT; BAI FU PIAN; BETAINE; BIOLOGICAL MARKER; CORTICOTROPIN; CYCLIC AMP; CYCLIC GMP; HYDROCORTISONE; METHIONINE; PHOSPHATE; PURINE NUCLEOTIDE; SEROTONIN RECEPTOR; TYROSINE; UNCLASSIFIED DRUG; AMINO ACID; PLANT EXTRACT;

AMINO ACID SYNTHESIS; ANALYSIS OF VARIANCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOINFORMATICS; BLOOD SAMPLING; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG MECHANISM; DRUG METABOLISM; KIDNEY YANG DEFICIENCY SYNDROME; LIQUID CHROMATOGRAPHY; MALE; MASS SPECTROMETRY; NONHUMAN; PARTIAL LEAST SQUARES REGRESSION; PRINCIPAL COMPONENT ANALYSIS; PROTEIN DEGRADATION; QUADRUPLE TIME OF FLIGHT MASS SPECTROMETRY; RADIOIMMUNOASSAY; RAT; SYNDROME; TIME OF FLIGHT MASS SPECTROMETRY; ACONITE; ANIMAL; BIOLOGY; CHEMISTRY; CHINESE MEDICINE; DISEASE MODEL; DOSE RESPONSE; KIDNEY DISEASES; METABOLISM; PATHOPHYSIOLOGY; PROCEDURES; SPRAGUE DAWLEY RAT; STATISTICAL MODEL; TOXICITY; YANG DEFICIENCY;

ACONITUM CARMICHAELI; RANUNCULACEAE; RATTUS;

ACONITUM; AMINO ACIDS; ANIMALS; BIOLOGICAL MARKERS; COMPUTATIONAL BIOLOGY; DISEASE MODELS, ANIMAL; DOSE-RESPONSE RELATIONSHIP, DRUG; HYDROCORTISONE; KIDNEY DISEASES; LINEAR MODELS; MALE; MASS SPECTROMETRY; MEDICINE, CHINESE TRADITIONAL; PLANT EXTRACTS; PRINCIPAL COMPONENT ANALYSIS; PURINE NUCLEOTIDES; RATS; RATS, SPRAGUE-DAWLEY; SYNDROME; YANG DEFICIENCY;

EID: 84900348841     PISSN: 03788741     EISSN: 18727573     Source Type: Journal    
DOI: 10.1016/j.jep.2014.02.011     Document Type: Article

References (78)

1. A. Ameri Effects of the Aconitum alkaloid mesaconitine in rat hippocampal slices and the involvement of alpha- and beta-adrenoceptors Br. J. Pharmacol. 123 1998 243 250
2. A. Ameri, and U. Seitz Effects of mesaconitine on [3H]noradrenaline uptake and neuronal excitability in rat hippocampus Exp. Brain. Res. 121 1998 451 456
3. S. Asano, S. Kamiya, and N. Takeguchi The energy transduction mechanism is different among P-type ion-transporting ATPases. Acetyl phosphate causes uncoupling between hydrolysis and ion transport in H+,K(+)-ATPase J. Biol. Chem. 267 1992 6590 6595
4. K. Bauer Carnosine and homocarnosine, the forgotten, enigmatic peptides of the brain Neurochem. Res. 30 2005 1339 1345
5. R.V. Carsia, G.J. Macdonald, and S. Malamed Steroid control of steroidogenesis in isolated adrenocortical cells: molecular and species specificity Steroids 41 1983 741 755
6. N.S. Carter, P. Yates, C.S. Arendt, J.M. Boitz, and B. Ullman Purine and pyrimidine metabolism in Leishmania Adv. Exp. Med. Biol. 625 2008 141 154
7. M. Chen, L. Zhao, and W. Jia Metabonomic study on the biochemical profiles of a hydrocortisone-induced animal model J. Proteome Res. 4 2005 2391 2396
8. Q. Chen Experimental Methodology of Pharmacological Research in Traditional Chinese Medicine 1993 Peoples Health Publishing House Beijing 982
9. V.N. Chesnokov, V.G. Gadzhiev, G.I. Leontyeva, S.M. Zelenin, and N.P. Mertvetsov Hydrocortisone regulation and expression of tyrosine aminotransferase gene in various tissues of rat Biomed. Biochim. Acta 49 1990 1177 1186
10. N.P. Committee China Pharmacopeia 2010 2010 China Medical Science Press Beijing
11. S.A. Craig Betaine in human nutrition Am. J. Clin. Nutr. 80 2004 539 549
12. Deng, Z.Z., He, Y.T., Yu, Y.M., 1996. [Comparison between two diagnostic methods of computers mathematic model and clinical diagnosis on TCM syndromes of rheumatoid arthritis]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi=Chin. J. Integr. Tradit. West. Med./Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 16, 727-729.
13. F.E. DiBenedetto, and L.Z. Bito The kinetics and energy dependence of prostaglandim transport processes. I. In vitro studies on the rate of PGF2 alpha accumulation by the rabbit anterior uvea Exp. Eye Res. 30 1980 175 182
14. L.S. Ding, F.E. Wu, and Y.Z. Chen [Diterpenoid alkaloids from Aconitum gymnandrum] Yao xue xue bao=Acta Pharm. Sin. 28 1993 188 191
15. F. Gao, Y.Y. Li, D. Wang, X. Huang, and Q. Liu Diterpenoid alkaloids from the Chinese traditional herbal "Fuzi" and their cytotoxic activity Molecules 17 2012 5187 5194
16. T. Gao, H. Bi, S. Ma, and J. Lu The antitumor and immunostimulating activities of water soluble polysaccharides from Radix Aconiti, Radix Aconiti Lateralis and Radix Aconiti Kusnezoffii Nat. Prod. Commun. 5 2010 447 455
17. Z.Z. Gizatullina, O.A. Sukocheva, and A.I. Gagelgans [Dose-dependent character of the effect of hydrocortisone on energy metabolism of rat thymocytes] Biokhimiia 61 1996 445 450
18. G.B. Grindey, J.K. Lowe, A.Y. Divekar, and M.T. Hakala Potentiation by guanine nucleosides of the growth-inhibitory effects of adenosine analogs on L1210 and sarcoma 180 cells in culture Cancer Res. 36 1976 379 383
19. W.L. Gu, Z.X. Shi, Y.X. Yu, Y.W. Wu, B.W. Lu, and K.K. Hui Distribution characteristics of syndrome types in essential hypertension Zhong xi yi jie he xue bao=J. Chin. Integr. Med. 8 2010 842 847
20. P.A. Guy, I. Tavazzi, S.J. Bruce, Z. Ramadan, and S. Kochhar Global metabolic profiling analysis on human urine by UPLC-TOFMS: issues and method validation in nutritional metabolomics J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 871 2008 253 260
21. A.R. Hipkiss, S.P. Cartwright, C. Bromley, S.R. Gross, and R.M. Bill Carnosine: can understanding its actions on energy metabolism and protein homeostasis inform its therapeutic potential? Chem. Cent. J. 7 2013 38
22. D. Huang, J. Yang, X. Lu, Y. Deng, Z. Xiong, and F. Li An integrated plasma and urinary metabonomic study using UHPLC-MS: intervention effects of Epimedium koreanum on 'Kidney-Yang Deficiency syndrome' rats J. Pharm. Biomed. Anal. 76 2013 200 206
23. Y. Ikeda, T. Oyama, and M. Taki Effect of processed Aconiti tuber on catecholamine and indoleamine contents in brain in rats Acta Anaesthesiol. Belg. 45 1994 113 118
24. T. Isono, T. Oyama, A. Asami, Y. Suzuki, Y. Hayakawa, Y. Ikeda, M. Noguchi, and Y. Omiya The analgesic mechanism of processed Aconiti tuber: the involvement of descending inhibitory system Am. J. Chin. Med. 22 1994 83 94
25. Jin, Y., Hu, S., Yan, D., 2000. [Study on molecular mechanism of ganyang shangkang syndrome in hypertension]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi=Chin. J. Integr. Tradit. West. Med./Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 20, 87-90.
26. R. Kawasaki, W. Motoya, T. Atsumi, C. Mouri, N. Kakiuchi, and M. Mikage The relationship between growth of the aerial part and alkaloid content variation in cultivated Aconitum carmichaeli Debeaux J. Nat. Med. 65 2011 111 115
27. D. Keszthelyi, F.J. Troost, and A.A. Masclee Understanding the role of tryptophan and serotonin metabolism in gastrointestinal function Neurogastroenterol. Motil.: Official J. Eur. Gastrointest. Motil. Soc. 21 2009 1239 1249
28. J. Kolanowski, and J. Crabbe Characteristics of the response of human adrenocortical cells to ACTH Mol. Cell. Endocrinol. 5 1976 255 267
29. D.V. Kratschmar, D. Calabrese, J. Walsh, A. Lister, J. Birk, C. Appenzeller-Herzog, P. Moulin, C.E. Goldring, and A. Odermatt Suppression of the Nrf2-dependent antioxidant response by glucocorticoids and 11beta-HSD1-mediated glucocorticoid activation in hepatic cells PloS one 7 2012 e36774
30. Lang, J.M., Li, M.Z., Wei, A.S., 1995. Study on objective parameters of syndrome differentiation of diabetic nephropathy. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi=Chin. J. Integr. Tradit. West. Med./Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 15, 84-86.
31. M. Lever, M.K. Storer, J.G. Lewis, P.M. George, and S.T. Chambers Plasma betaine concentrations correlate with plasma cortisol but not with C-reactive protein in an elderly population Clin. Chem. Lab. Med.: CCLM/FESCC 50 2012 1635 1640
32. X. Liang, X. Chen, Q. Liang, H. Zhang, P. Hu, Y. Wang, and G. Luo Metabonomic study of Chinese medicine Shuanglong formula as an effective treatment for myocardial infarction in rats J. Proteome Res. 10 2011 790 799
33. L. Liu, B. Li, Y. Zhou, L. Wang, F. Tang, D. Shao, X. Jiang, H. Zhao, R. Cui, and Y. Li Antidepressant-like effect of Fuzi total alkaloid on ovariectomized mice J. Pharmacol. Sci. 120 2012 280 287
34. X. Liu, J. Du, J. Cai, G. Xu, A. Lin, and Q. Teng Clinical systematic observation of Kangxin capsule curing vascular dementia of senile kidney deficiency and blood stagnation type J. Ethnopharmacol. 112 2007 350 355
35. A.P. Lu, X.W. Liu, and X.R. Ding Methodology of pharmacodynamic evaluation on Chinese herbal medicine based on syndrome differentiation Zhong xi yi jie he xue bao=J. Chin. Integr. Med. 7 2009 501 504
36. X. Lu, Z. Xiong, J. Li, S. Zheng, T. Huo, and F. Li Metabonomic study on 'Kidney-Yang Deficiency syndrome' and intervention effects of Rhizoma Drynariae extracts in rats using ultra performance liquid chromatography coupled with mass spectrometry Talanta 83 2011 700 708
37. Y. Lv, X. Liu, S. Yan, X. Liang, Y. Yang, W. Dai, and W. Zhang Metabolomic study of myocardial ischemia and intervention effects of Compound Danshen Tablets in rats using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry J. Pharm. Biomed. Anal. 52 2010 129 135
38. M.P. Maguire, K.R. Sheets, K. McVety, A.P. Spada, and A. Zilberstein A new series of PDGF receptor tyrosine kinase inhibitors: 3-substituted quinoline derivatives J. Med. Chem. 37 1994 2129 2137
39. C. Morin, R. Zini, N. Simon, P. Charbonnier, J.P. Tillement, and H. Le Louet Low glucocorticoid concentrations decrease oxidative phosphorylation of isolated rat brain mitochondria: an additional effect of dexamethasone Fundam. Clin. Pharmacol. 14 2000 493 500
40. T. Nakagohri, T. Asano, T. Uematsu, T. Kenmochi, T. Kubota, O. Kainuma, and K. Isono The effects of prostaglandin E1 and tyrosine kinase inhibitors on energy status and protein synthetic ability in hepatic ischemia-reperfusion injury Surg. Today 28 1998 517 521
41. R.J. Porter, R.H. McAllister-Williams, B.S. Lunn, and A.H. Young 5-Hydroxytryptamine receptor function in humans is reduced by acute administration of hydrocortisone Psychopharmacology 139 1998 243 250
42. Y. Qiu, M. Chen, M. Su, G. Xie, X. Li, M. Zhou, A. Zhao, J. Jiang, and W. Jia Metabolic profiling reveals therapeutic effects of Herba Cistanches in an animal model of hydrocortisone-induced 'kidney-deficiency syndrome Chin. Med. 3 2008 3
43. D.Y. Rhyu, K.S. Kang, M. Sekiya, and T. Yokozawa Antioxidant effect of Wen-Pi-Tang and its component crude drugs on oxidative stress Am. J. Chin. Med. 35 2007 127 137
44. E.C. Schmidt, and R.M. Goodman Hormone-induced alterations in nonhistone protein. Methylation and phosphorylation in Sciara coprophila Differentiation Res. Biol. Divers. 6 1976 177 186
45. Sepiashvili, R.I., Balmasova, I.P., Staurina, L.N., 2013. Serotonin and its immune and physiological effects. Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova/Rossiiskaia akademiia nauk 99, 17-32.
46. H. Sheridan, L. Krenn, R. Jiang, I. Sutherland, S. Ignatova, A. Marmann, X. Liang, and J. Sendker The potential of metabolic fingerprinting as a tool for the modernisation of TCM preparations J. Ethnopharmacol. 140 2012 482 491
47. J. Singhuber, M. Zhu, S. Prinz, and B. Kopp Aconitum in traditional Chinese medicine: a valuable drug or an unpredictable risk? J. Ethnopharmacol. 126 2009 18 30
48. V.S. Slyshenkov, D. Dymkowska, and L. Wojtczak Pantothenic acid and pantothenol increase biosynthesis of glutathione by boosting cell energetics FEBS Lett. 569 2004 169 172
49. W. Su, A.X. Hu, H. Du, K. Yuan, and H.F. Xu Effects of Yiniao Recipe on serum antidiuretic hormone level and plasma ratio of cAMP to cGMP in rats with kidney-yang deficiency Zhong xi yi jie he xue bao=J. Chin. Integr. Med. 8 2010 168 172
50. K. Suhre, S.Y. Shin, A.K. Petersen, R.P. Mohney, D. Meredith, B. Wagele, E. Altmaier, P. Deloukas, J. Erdmann, E. Grundberg, C.J. Hammond, M.H. de Angelis, G. Kastenmuller, A. Kottgen, F. Kronenberg, M. Mangino, C. Meisinger, T. Meitinger, H.W. Mewes, M.V. Milburn, C. Prehn, J. Raffler, J.S. Ried, W. Romisch-Margl, N.J. Samani, K.S. Small, H.E. Wichmann, G. Zhai, T. Illig, T.D. Spector, J. Adamski, N. Soranzo, and C. Gieger Human metabolic individuality in biomedical and pharmaceutical research Nature 477 2011 54 60
51. Sun, S., Dai, J., Wang, W., Cao, H., Fang, J., Hu, Y.Y., Su, S., Zhang, Y., 2012a. Metabonomic evaluation of ZHENG differentiation and treatment by Fuzhenghuayu tablet in hepatitis-b-caused cirrhosis. Evidence-based Complementary and Alternative Medicine: eCAM 2012, 453503.
52. Sun, Z.Y., Zhao, Y.L., Wang, J.B., Zhang, L., Wei, S.S., Jiang, F.J., Jia, L., Cheng, D.H., Xiao, X.H., 2012b. Research on fuzi based on animal thermotropism behavior to discover if it has fewer "hot" characteristics without Ganjiang. J. Tradit. Chin. Med.=Chung i tsa chih ying wen pan / sponsored by All-China Association of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine 32, 208-214.
53. Y.W. Teng, J.M. Ellis, R.A. Coleman, and S.H. Zeisel Mouse betaine-homocysteine S-methyltransferase deficiency reduces body fat via increasing energy expenditure and impairing lipid synthesis and enhancing glucose oxidation in white adipose tissue J. Biol. Chem. 287 2012 16187 16198
54. A.S. Tischler, R.L. Perlman, G.M. Morse, and B.E. Sheard Glucocorticoids increase catecholamine synthesis and storage in PC12 pheochromocytoma cell cultures J. Neurochem. 40 1983 364 370
55. H. Tsutsui, T. Maeda, J.Z. Min, S. Inagaki, T. Higashi, Y. Kagawa, and T. Toyooka Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry Clin. Chim. Acta 412 2011 861 872
56. H. Uzuner, R. Bauer, T.P. Fan, D.A. Guo, A. Dias, H. El-Nezami, T. Efferth, E.M. Williamson, M. Heinrich, N. Robinson, P.J. Hylands, B.M. Hendry, Y.C. Cheng, and Q. Xu Traditional Chinese medicine research in the post-genomic era: good practice, priorities, challenges and opportunities J. Ethnopharmacol. 140 2012 458 468
57. K. Van den Bergh, M. Vercammen, S. Regenass, R. Derua, P. Vermeersch, P. Pokreisz, A. Ocmant, K.O. de Beeck, S. Janssens, E. Waelkens, and X. Bossuyt Betaine homocysteine methyl transferase 1, a novel auto-antigen associated with anti-Golgi immune reactivity Clin. Chim. Acta 413 2012 105 108
58. C. van Waveren, and C.T. Moraes Transcriptional co-expression and co-regulation of genes coding for components of the oxidative phosphorylation system BMC Genomics 9 2008 18
59. J.G. Wang, L. Pan, B. Wu, and M. Wang Familial characteristics of kidney-yang deficiency and cold syndrome J. Toxicol. Environ. Health A 69 2006 1939 1950
60. R. Wang, A.Z. Xiong, Z.Q. Teng, Q.W. Yang, Y.H. Shi, and L. Yang Radix Paeoniae Rubra and Radix Paeoniae Alba attenuate CCl4-induced acute liver injury: an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) based metabolomic approach for the pharmacodynamic study of traditional Chinese medicines (TCMs) Int. J. Mol. Sci. 13 2012 14634 14647
61. X. Wang, H. Sun, A. Zhang, W. Sun, P. Wang, and Z. Wang Potential role of metabolomics apporoaches in the area of traditional Chinese medicine: as pillars of the bridge between Chinese and Western medicine J. Pharm. Biomed. Anal. 55 2011 859 868
62. X. Wang, A. Zhang, H. Sun, and P. Wang Systems biology technologies enable personalized traditional Chinese medicine: a systematic review Am. J. Chin. Med. 40 2012 1109 1122
63. T.H. Welsh Jr., T.H. Bambino, and A.J. Hsueh Mechanism of glucocorticoid-induced suppression of testicular androgen biosynthesis in vitro Biol. Reprod. 27 1982 1138 1146
64. L. Xiang, P. Jiang, C. Zhan, Z. Chen, X. Liu, X. Huang, S. Wang, Y. Hu, W. Zhang, and R. Liu The serum metabolomic study of intervention effects of the traditional Chinese medicine Shexiang Baoxin Pill and a multi-component medicine polypill in the treatment of myocardial infarction in rats Mol. Biosyst. 8 2012 2434 2442
65. C. Xie, D. Zhong, K. Yu, and X. Chen Recent advances in metabolite identification and quantitative bioanalysis by LC-Q-TOF MS Bioanalysis 4 2012 937 959
66. G.Z. Xin, L.W. Qi, Z.Q. Shi, P. Li, H.P. Hao, G.J. Wang, and J. Shang Strategies for integral metabolism profile of multiple compounds in herbal medicines: pharmacokinetics, metabolites characterization and metabolic interactions Curr. Drug Metab. 12 2011 809 817
67. P.H. Xiong, L. Zhang, S.F. Tian, M.H. Gu, and A.P. Chen Effect of Equiguard in treating patients with Shen-yang deficiency syndrome Chin. J. Integr. Med. 14 2008 225 227
68. Yin, G.Y., Yin, Y.F., He, X.F., 1995. Effect of zhuchun pill on immunity and endocrine function of elderly with kidney-yang deficiency. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi=Chin. J. Integr. Tradit. West. Med./Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 15, 601-603.
69. A.H. Young, A.L. Sharpley, G.M. Campling, R.A. Hockney, and P.J. Cowen Effects of hydrocortisone on brain 5-HT function and sleep J. Affect. Disord. 32 1994 139 146
70. S.N. Young Mechanism of decline in rat brain 5-hydroxytryptamine after induction of liver tryptophan pyrrolase by hydrocortisone: roles of tryptophan catabolism and kynurenine synthesis Br. J. Pharmacol. 74 1981 695 700
71. H.Y. Yu, S.J. Wang, J.L. Teng, X.M. Ji, Z.C. Wu, Q.C. Ma, and X.J. Fu Effects of Radix aconiti lateralis preparata and Rhizoma zingiberis on energy metabolism and expression of the genes related to metabolism in rats Chin. J. Integr. Med. 18 2012 23 29
72. S.H. Zeisel Metabolic crosstalk between choline/1-carbon metabolism and energy homeostasis Clin. Chem. Lab. Med.: CCLM/FESCC 51 2013 467 475
73. S.H. Zeisel, and K.A. da Costa Choline: an essential nutrient for public health Nutr. Rev. 67 2009 615 623
74. A. Zhang, H. Sun, Z. Wang, W. Sun, P. Wang, and X. Wang Metabolomics: towards understanding traditional Chinese medicine Planta Med. 76 2010 2026 2035
75. D. Zhao, J. Wang, Y. Cui, and X. Wu Pharmacological effects of Chinese herb aconite (fuzi) on cardiovascular system J. Tradit. Chin. Med.=Chung i tsa chih ying wen pan/sponsored by All-China Association of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine 32 2012 308 313
76. Zhao, H., Li, J., He, X., Lu, C., Xiao, C., Niu, X., Zhao, N., Ju, D., Lu, A., 2012b. The protective effect of yi shen juan bi pill in arthritic rats with castration-induced kidney deficiency. Evidence-based complementary and alternative medicine: eCAM 2012, 102641.
77. X. Zhao, Z. Long, J. Dai, K. Bi, and X. Chen Identification of multiple constituents in the traditional Chinese medicine formula Zhi-zi-chi decoction and rat plasma after oral administration by liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry Rapid Commun. Mass Spectrom. RCM 26 2012 2443 2453
78. Z.D. Zou, N. Liu, P. Guo, L.Y. Guo, Y. Sun, J. Shi, and L. Wang Analysis on clinical treatment in hypertension by traditional Chinese medicine for 10 years in Beijing Zhongguo Zhong yao za zhi=Zhongguo zhongyao zazhi=China J. Chin. Materia Medica 32 2007 1569 1572