Group I PAK inhibitor IPA-3 induces cell death and affects cell adhesivity to fibronectin in human hematopoietic cells

PLoS ONE

Volumn 9, Issue 3, 2014, Pages

  Kuželová, Kateřina ^a   Grebeňová, Dana ^a   Holoubek, Aleš ^a   Röselová, Pavla ^a   Obr, Adam ^a  

^a INSTITUTE OF HEMATOLOGY AND BLOOD TRANSFUSION   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; COFILIN; FIBRONECTIN; IPA 3; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; P21 ACTIVATED KINASE 1; PROTEIN SERINE THREONINE KINASE INHIBITOR; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; ACTIN DEPOLYMERIZING FACTOR; DISULFIDE; IPA-3 COMPOUND; NAPHTHOL DERIVATIVE; P21 ACTIVATED KINASE; PROTEIN KINASE INHIBITOR; SERINE;

ARTICLE; CELL ADHESION; CELL DEATH; CELL MIGRATION; CELL PROLIFERATION; CML T1 CELL LINE; CONTROLLED STUDY; DNA FRAGMENTATION; DOWNSTREAM PROCESSING; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; HEL CELL LINE; HEMATOPOIETIC CELL; HL 60 CELL LINE; HUMAN; HUMAN CELL; IMPEDANCE; INTERFERENCE REFLECTION MICROSCOPY; JURKAT CELL LINE; JURL MK1 CELL LINE; K562 CELL LINE; KARPAS 299 CELL LINE; LEUKEMIA CELL LINE; MICROSCOPY; MOLM 7 CELL LINE; PROTEIN CLEAVAGE; PROTEIN DEPHOSPHORYLATION; ANTAGONISTS AND INHIBITORS; APOPTOSIS; BLOOD CELL; CHEMISTRY; CYTOLOGY; DEFICIENCY; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; HEMATOPOIESIS; INTRACELLULAR SPACE; LEUKEMIA; LYMPHOMA; METABOLISM; PATHOLOGY; PHOSPHORYLATION;

ACTIN DEPOLYMERIZING FACTORS; APOPTOSIS; BLOOD CELLS; CELL ADHESION; CELL PROLIFERATION; DISULFIDES; FIBRONECTINS; GENE EXPRESSION REGULATION; GENE SILENCING; HEMATOPOIESIS; HUMANS; INTRACELLULAR SPACE; LEUKEMIA; LYMPHOMA; NAPHTHOLS; P21-ACTIVATED KINASES; PHOSPHORYLATION; PROTEIN KINASE INHIBITORS; RNA, SMALL INTERFERING; SERINE;

EID: 84899769797     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0092560     Document Type: Article

References (44)

1. Arias-Romero LE, Chernoff J (2008) A tale of two paks. Biol. Cell. 100: 97-108. (Pubitemid 351266767)
2. Kichina JV, Goc A, Al-Husein B, Somanath PR, Kandel ES (2010) PAK1 as a therapeutic target. Expert Opin. Ther. Targets 14: 703-725.
3. Coniglio SJ, Zavarella S, Symons MH (2008) Pak1 and Pak2 mediate tumor cell invasion through distinct signaling mechanisms. Mol. Cell. Biol. 28: 4162-4172. (Pubitemid 351812999)
4. Arai A, Jin A, Yan W, Mizuchi D, Yamamoto K, et al. (2005) SDF-1 synergistically enhances IL-3-induced activation of the raf-1/MEK/Erk signaling pathway through activation of rac and its effector pak kinases to promote hematopoiesis and chemotaxis. Cell. Signal. 17: 497-506. (Pubitemid 39643067)
5. Wang RA, Zhang H, Balasenthil S, Medina D, Kumar R (2006) PAK1 hyperactivation is sufficient for mammary gland tumor formation. Oncogene 25: 2931-2936. (Pubitemid 43739243)
6. Parekh P, Rao KV (2007) Overexpression of cyclin D1 is associated with elevated levels of MAP kinases, akt and Pak1 during diethylnitrosamine-induced progressive liver carcinogenesis. Cell Biol. Int. 31: 35-43. (Pubitemid 46124028)
7. Kamai T, Shirataki H, Nakanishi K, Furuya N, Kambara T, et al. (2010) Increased Rac1 activity and Pak1 overexpression are associated with lymphovascular invasion and lymph node metastasis of upper urinary tract cancer. BMC Cancer 10: 164-2407-10-164.
8. McCarty SK, Saji M, Zhang X, Jarjoura D, Fusco A, et al. (2010) Group I p21-activated kinases regulate thyroid cancer cell migration and are overexpressed and activated in thyroid cancer invasion. Endocr. Relat. Cancer 17: 989-999.
9. Lee MY, Kim SH, Ihm HJ, Chae HD, Kim CH, et al. (2011) Up-regulation of p21-activated kinase 1 by in vitro treatment with interleukin 1-beta and its increased expression in ovarian endometriotic cysts. Fertil. Steril. 96: 508-511.
10. Ong CC, Jubb AM, Haverty PM, Zhou W, Tran V, et al. (2011) Targeting p21-activated kinase 1 (PAK1) to induce apoptosis of tumor cells. Proc. Natl. Acad. Sci. U. S. A. 108: 7177-7182.
11. Sato M, Matsuda Y, Wakai T, Kubota M, Osawa M, et al. (2013) P21-activated kinase-2 is a critical mediator of transforming growth factor-beta-induced hepatoma cell migration. J. Gastroenterol. Hepatol.
12. Ong CC, Jubb AM, Jakubiak D, Zhou W, Rudolph J, et al. (2013) P21-activated kinase 1 (PAK1) as a therapeutic target in BRAF wild-type melanoma. J. Natl. Cancer Inst.
13. Hoover WC, Zhang W, Xue Z, Gao H, Chernoff J, et al. (2012) Inhibition of p21 activated kinase (PAK) reduces airway responsiveness in vivo and in vitro in murine and human airways. PLoS One 7: e42601.
14. Stockton RA, Schaefer E, Schwartz MA (2004) P21-activated kinase regulates endothelial permeability through modulation of contractility. J. Biol. Chem. 279: 46621-46630. (Pubitemid 39518307)
15. Walsh K, McKinney MS, Love C, Liu Q, Fan A, et al. (2013) PAK1 mediates resistance to PI3K inhibition in lymphomas. Clin. Cancer Res. 19: 1106-1115.
16. Deacon SW, Beeser A, Fukui JA, Rennefahrt UE, Myers C, et al. (2008) An isoform-selective, small-molecule inhibitor targets the autoregulatory mechanism of p21-activated kinase. Chem. Biol. 15: 322-331. (Pubitemid 351508427)
17. Flaiz C, Chernoff J, Ammoun S, Peterson JR, Hanemann CO (2009) PAK kinase regulates rac GTPase and is a potential target in human schwannomas. Exp. Neurol. 218: 137-144.
18. Viaud J, Peterson JR (2009) An allosteric kinase inhibitor binds the p21-activated kinase autoregulatory domain covalently. Mol. Cancer. Ther. 8: 2559-2565.
19. Grebenova D, Roselova P, Pluskalova M, Halada P, Rosel D, et al. (2012) Proteins implicated in the increase of adhesivity induced by suberoylanilide hydroxamic acid in leukemic cells. J. Proteomics.
20. Dorrance AM, De Vita S, Radu M, Reddy PN, McGuinness MK, et al. (2013) The rac GTPase effector p21-activated kinase is essential for hematopoietic stem/progenitor cell migration and engraftment. Blood 121: 2474-2482.
21. Tsuji-Takayama K, Kamiya T, Nakamura S, Matsuo Y, Adachi T, et al. (1994) Establishment of multiple leukemia cell lines with diverse myeloid and/or megakaryoblastoid characteristics from a single Ph1 positive chronic myelogenous leukemia blood sample. Hum. Cell 7: 167-171.
22. Martin P, Papayannopoulou T (1982) HEL cells: A new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233-1235. (Pubitemid 12080055)
23. Kuzelova K, Grebenova D, Hrkal Z (2007) Labeling of apoptotic JURL-MK1 cells by fluorescent caspase-3 inhibitor FAM-DEVD-fmk occurs mainly at site(s) different from caspase-3 active site. Cytometry A. 71: 605-611. (Pubitemid 47221723)
24. Kuzelova K, Pluskalova M, Brodska B, Otevrelova P, Elknerova K, et al. (2010) Suberoylanilide hydroxamic acid (SAHA) at subtoxic concentrations increases the adhesivity of human leukemic cells to fibronectin. J. Cell. Biochem. 109: 184-195.
25. Obr A, Roselova P, Grebenova D, Kuzelova K (2013) Real-time monitoring of hematopoietic cell interaction with fibronectin fragment: The effect of histone deacetylase inhibitors. Cell. Adh Migr. 7: 275-282.
26. Persico A, Cervigni RI, Barretta ML, Corda D, Colanzi A (2010) Golgi partitioning controls mitotic entry through aurora-A kinase. Mol. Biol. Cell 21: 3708-3721.
27. Barr VA, Bunnell SC (2009) Interference reflection microscopy. Curr. Protoc. Cell. Biol. Chapter 4: Unit 4.23.
28. Singhal R, Kandel ES (2012) The response to PAK1 inhibitor IPA3 distinguishes between cancer cells with mutations in BRAF and ras oncogenes. Oncotarget 3: 700-708.
29. Bright MD, Garner AP, Ridley AJ (2009) PAK1 and PAK2 have different roles in HGF-induced morphological responses. Cell. Signal. 21: 1738-1747.
30. Luo S, Rubinsztein DC (2009) Huntingtin promotes cell survival by preventing Pak2 cleavage. J. Cell. Sci. 122: 875-885.
31. Siu MK, Wong ES, Chan HY, Kong DS, Woo NW, et al. (2010) Differential expression and phosphorylation of Pak1 and Pak2 in ovarian cancer: Effects on prognosis and cell invasion. Int. J. Cancer 127: 21-31.
32. Wu R, Abramson AL, Symons MH, Steinberg BM (2010) Pak1 and Pak2 are activated in recurrent respiratory papillomas, contributing to one pathway of Rac1-mediated COX-2 expression. Int. J. Cancer 127: 2230-2237.
33. Marlin JW, Chang YW, Ober M, Handy A, Xu W, Jakobi R (2011) Functional PAK-2 knockout and replacement with a caspase cleavage-deficient mutant in mice reveals differential requirements of full-length PAK-2 and caspase-activated PAK-2p34. Mamm. Genome 22: 306-317.
34. Li T, Zhang J, Zhu F, Wen W, Zykova T, et al. (2011) P21-activated protein kinase (PAK2)-mediated c-jun phosphorylation at 5 threonine sites promotes cell transformation. Carcinogenesis 32: 659-666.
35. Lee N, MacDonald H, Reinhard C, Halenbeck R, Roulston A, et al. (1997) Activation of hPAK65 by caspase cleavage induces some of the morphological and biochemical changes of apoptosis. Proc. Natl. Acad. Sci. U. S. A. 94: 13642-13647. (Pubitemid 28009636)
36. Rudel T, Bokoch GM (1997) Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2. Science 276: 1571-1574. (Pubitemid 27255323)
37. Kuzelova K, Grebenova D, Brodska B (2011) Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes. J. Cell. Biochem. 112: 3334-3342.
38. Yang L, Wang L, Geiger H, Cancelas JA, Mo J, Zheng Y (2007) Rho GTPase Cdc42 coordinates hematopoietic stem cell quiescence and niche interaction in the bone marrow. Proc. Natl. Acad. Sci. U. S. A. 104: 5091-5096. (Pubitemid 47186181)
39. Tybulewicz VL, Henderson RB (2009) Rho family GTPases and their regulators in lymphocytes. Nat. Rev. Immunol. 9: 630-644.
40. Mulloy JC, Cancelas JA, Filippi MD, Kalfa TA, Guo F, Zheng Y (2010) Rho GTPases in hematopoiesis and hemopathies. Blood 115: 936-947.
41. Wang H, Zeng X, Fan Z, Lim B (2010) RhoH plays distinct roles in T-cell migrations induced by different doses of SDF1 alpha. Cell. Signal. 22: 1022-1032.
42. Daub H, Gevaert K, Vandekerckhove J, Sobel A, Hall A (2001) Rac/Cdc42 and p65PAK regulate the microtubule-destabilizing protein stathmin through phosphorylation at serine 16. J. Biol. Chem. 276: 1677-1680. (Pubitemid 32109635)
43. Wittmann T, Bokoch GM, Waterman-Storer CM (2004) Regulation of microtubule destabilizing activity of Op18/stathmin downstream of Rac1. J. Biol. Chem. 279: 6196-6203. (Pubitemid 38220653)
44. Kosoff R, Chow HY, Radu M, Chernoff J (2013) Pak2 kinase restrains mast cell FcepsilonRI receptor signaling through modulation of rho protein guanine nucleotide exchange factor (GEF) activity. J. Biol. Chem. 288: 974-983.