Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2

Molecular Medicine Reports

Volumn 9, Issue 6, 2014, Pages 2505-2511

  Liu, Ling ^a   Zhang, Jingkai ^a   Li, Meiling ^a   Zhang, Xiaohong ^a   Zhang, Jinlu ^a   Li, Zhenjing ^a   Wang, Likui ^b   Wu, Jihui ^c   Luo, Cheng ^a  

^a TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b CAPITAL MEDICAL UNIVERSITY   (China)

^c UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

Anti inflammation; Antioxidation; COX 2; HepG2 cell; Polysaccharide; Ursolic acid

Indexed keywords

ASTRAGALUS POLYSACCHARIDE; CYCLOOXYGENASE 2; EXOPOLYSACCHARIDE; FATTY ACID; MALONALDEHYDE; POLYSACCHARIDE; PROSTAGLANDIN E2; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG; URSOLIC ACID; VIILI EXOPOLYSACCHARIDE; PROSTAGLANDIN SYNTHASE INHIBITOR; REACTIVE OXYGEN METABOLITE; TRITERPENE;

ANTINEOPLASTIC ACTIVITY; ANTIOXIDANT RESPONSIVE ELEMENT; ANTIPROLIFERATIVE ACTIVITY; APOPTOSIS; ARTICLE; ASTRAGALUS PLANT; CELL CYCLE ARREST; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; DRUG MECHANISM; DRUG POTENTIATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HEPG2 CELL LINE; HUMAN; HUMAN CELL; LIPID PEROXIDATION; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCANNING ELECTRON MICROSCOPY; WESTERN BLOTTING; CELL CYCLE CHECKPOINT; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETICS; METABOLISM;

APOPTOSIS; CELL CYCLE CHECKPOINTS; CELL PROLIFERATION; CYCLOOXYGENASE 2; CYCLOOXYGENASE INHIBITORS; DINOPROSTONE; DOWN-REGULATION; GENE EXPRESSION REGULATION, NEOPLASTIC; HEP G2 CELLS; HUMANS; POLYSACCHARIDES; REACTIVE OXYGEN SPECIES; TRITERPENES;

EID: 84899699515     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2014.2059     Document Type: Article

References (33)

1. Lodato F, Mazzella G, Festi D, Azzaroli F, Colecchia A and Roda E: Hepatocellular carcinoma prevention: a worldwide emergence between the opulence of developed countries and the economic constraints of developing nations. World J Gastroenterol 12: 7239-7249, 2006. (Pubitemid 44911828)
2. El-Serag HB: Epidemiology of hepatocellular carcinoma. Clin Liver Dis 5: 87-107, 2001.
3. Goodgame B, Shaheen NJ, Galanko J and El-Serag HB: The risk of end stage liver disease and hepatocellular carcinoma among persons infected with hepatitis C virus: publication bias? Am J Gastroenterol 98: 2535-2342, 2003.
4. Simonetti RG, Camm̀ C, Fiorello F, Politi F, D'Amico G and Pagliaro L: Hepatocellular carcinoma: a worldwide problem and the major risk factors. Dig Dis Sci 36: 962-972, 1991.
5. Liu J: Oleanolic acid and ursolic acid: research perspectives. J Ethnopharmacol 100: 92-94, 2005.
6. Aggarwal BB and Shishodia S: Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol 71: 1397-1421, 2006.
7. Hsu YL, Kuo PL and Lin CC: Proliferative inhibition, cellcycle dysregulation, and induction of apoptosis by ursolic acid in human non-small cell lung cancer A549 cells. Life Sci 75: 2303-2316, 2004.
8. Choi BM, Park R, Pae HO, et al: Cyclic adenosine monophosphate inhibits ursolic acid-induced apoptosis via activation of protein kinase A in human leukaemic HL-60 cells. Pharmacol Toxicol 86: 53-58, 2000. (Pubitemid 30106349)
9. Choi YH, Baek JH, Yoo MA, et al: Induction of apoptosis by ursolic acid through activation of caspases and down-regulation of c-IAPs in human prostate epithelial cells. Int J Oncol 17: 565-571, 2000.
10. Huang MT, Ho CT, Wang ZY, et al: Inhibition of skin tumorigenesis by rosemary and its constituents carnosol and ursolic acid. Cancer Res 54: 701-708, 1994. (Pubitemid 24068325)
11. Nishino H, Nishino A, Takayasu J, et al: Inhibition of the tumor-promoting action of 12-O-tetradecanoylphorbol-13-acetate by some oleanane-type triterpenoid compounds. Cancer Res 48: 5210-5215, 1988.
12. Cha HJ, Bae SK, Lee HY, et al: Anti-invasive activity of ursolic acid correlates with the reduced expression of matrix metalloproteinase-9 (MMP-9) in HT1080 human fibrosarcoma cells. Cancer Res 56: 2281-2284, 1996.
13. Saxelin ML, Nurmiaho-Lassila EL, Meriläinen VT and Forsén RI: Ultrastructure and host specificty of bacteriophages of Streptococcus cremoris, Streptococcus lactis subsp. diacetylactis, and Leuconostoc cremoris from Finnish fermented milk 'viili'. Appl Environ Microbiol 52: 771-777, 1986.
14. Liu L, Wu J, Zhang J, et al: A compatibility assay of ursolic acid and foodborne microbial exopolysaccharides by antioxidant power and anti-proliferative properties in hepatocarcinoma cells. J Food Agric Environ 10: 111-114, 2012.
15. Kitazawa H, Yamaguchi T and Itoh T: B-cell mitogenic activity of slime products produced from slime-forming, encapsulated Lactococcus lactis ssp. cremoris. J Dairy Sci 75: 2946-2951, 1992.
16. Shao BM, Xu W, Dai H, et al: A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. Biochem Biophys Res Commun 320: 1103-1111, 2004. (Pubitemid 38916657)
17. Ross R: Atherosclerosis - an inflammatory disease. N Eng J Med 340: 115-126, 1999.
18. Akhtar M, Cheng Y, Magno RM, et al: Promoter methylation regulates Helicobacter pylori-stimulated cyclooxygenase-2 expression in gastric epithelial cells. Cancer Res 61: 2399-2403, 2001. (Pubitemid 32685813)
19. Kim H, Lim JW and Kim KH: Helicobacter pylori-induced expression of interleukin-8 and cyclooxygenase-2 in AGS gastric epithelial cells: mediation by NF-kappaB. Scand J Gastroenterol 36: 706-716, 2001. (Pubitemid 32568548)
20. Shariat SF, Kim JH, Ayala GE, et al: Cyclooxygenase-2 is highly expressed in carcinoma in situ and T1 transitional cell carcinoma of the bladder. J Urol 169: 938-942, 2003. (Pubitemid 36188129)
21. Leng J, Han C, Demetris AJ, et al: Cyclooxygenase-2 promotes hepatocellular carcinoma cell growth through Akt activation: evidence for Akt inhibition in celecoxib-induced apoptosis. Hepatology 38: 756-768, 2003. (Pubitemid 37052162)
22. Baek JY, Hur W, Wang JS, et al: Selective COX-2 inhibitor, NS-398, suppresses cellular proliferation in human hepatocellular carcinoma cell lines via cell cycle arrest. World J Gastroenterol 13: 1175-1181, 2007. (Pubitemid 46592957)
23. Mantovani A, Allavena P, Sica A and Balkwill F: Cancer-related inflammation. Nature 454: 436-444, 2008.
24. Coussens LM and Werb Z: Inflammation and cancer. Nature 420: 860-867, 2002. (Pubitemid 36019639)
25. Oztürk HS, Karayvaz M, Kaçmaz M, et al: Activities of the enzymes participating in purine and free-radical metabolism in cancerous human colorectal tissues. Cancer Biochem Biophs 16: 157-168, 1998. (Pubitemid 128701528)
26. Eapen CE, Madesh M, Balasubramanian KA, et al: Mucosal mitochondirial function and antioxidation defences in patients with gastric carcinoma. Scand J Gastroenterol 33: 975-981, 1998. (Pubitemid 28439316)
27. Luo C, Urgard E, Vooder T and Metspalu A: The role of COX-2 and Nrf2/ARE in anti-inflammation and antioxidative stress: Ageing and anti-ageing. Med Hypotheses 77: 174-178, 2011.
28. Chen C: COX-2's new role in inflammation. Nat Chem Biol 6: 401-402, 2010.
29. Groeger AL, Cipollina C, Cole MP, Woodcock SR, et al: Cyclooxygenase-2 generates anti-inflammatory mediators from omega-3 fatty acids. Nat Chem Biol 6: 433-441, 2010.
30. Kerr JF, Winterford CM and Harmon BV: Apoptosis. Its significance in cancer and cancer therapy. Cancer 73: 2013-2026, 1994. (Pubitemid 24107952)
31. Elstein KH and Zucker RM: Comparison of cellular and nuclear flow-cytometric techniques for discriminating apoptotic subpopulations. Exp Cell Res 211: 322-331, 1994. (Pubitemid 24174964)
32. Lebeer S, Claes IJ, Verhoeven TL, et al: Exopolysaccharides of Lactobacillus rhamnosus GG form a protective shield against innateimmune factors in the intestine. Microb Biotechnol 4: 368-374, 2011.
33. Goodman MT, Wu AH, Tung KH, et al: Association of dairy products, lactose, and calcium with the risk of ovarian cancer. Am J Epidemiol 156: 148-157, 2002. (Pubitemid 34755953)