COLD-PCR amplification of bisulfite-converted DNA allows the enrichment and sequencing of rare un-methylated genomic regions

PLoS ONE

Volumn 9, Issue 4, 2014, Pages

  Castellanos Rizaldos, Elena ^a   Milbury, Coren A ^a   Karatza, Elli ^a   Chen, Clark C ^b   Makrigiorgos, G Mike ^a   Merewood, Anne ^c  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c BOSTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BISULFITE; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; DNA; HYDROGEN SULFITE; SULFITE;

ARTICLE; CLINICAL ARTICLE; COAMPLIFICATION AT LOWER DENATURATION TEMPERATURE POLYMERASE CHAIN REACTION; CONTROLLED STUDY; DNA DENATURATION; DNA METHYLATION; DNA SEQUENCE; GLIOMA; HUMAN; HUMAN TISSUE; INFANT; O 6 METHYL GUANINE DNA METHYLTRANSFERASE GENE; POLYMERASE CHAIN REACTION; PROMOTER REGION; TEMPERATURE; CHEMISTRY; PROCEDURES;

DNA; DNA METHYLATION; POLYMERASE CHAIN REACTION; SULFITES;

EID: 84899618281     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0094103     Document Type: Article

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