COLD-PCR: Improving the sensitivity of molecular diagnostics assays

Expert Review of Molecular Diagnostics

Volumn 11, Issue 2, 2011, Pages 159-169

  Milbury, Coren A ^a   Li, Jin ^a   Liu, Pingfang ^a   Makrigiorgos, G Mike ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

cancer; coamplification at lower denaturation temperature; COLD PCR; denaturation temperature; low abundance mutations; variant and mutation enrichment

Indexed keywords

DNA; DOUBLE STRANDED DNA;

ALLELE; AMPLICON; COAMPLIFICATION AT LOWER DENATURATION TEMPERATURE POLYMERASE CHAIN REACTION; DENATURING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; DNA SEQUENCE; GENE MUTATION; GENETIC VARIABILITY; GENOTYPE; HUMAN; LOW TEMPERATURE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR DIAGNOSIS; POLYMERASE CHAIN REACTION; PYROSEQUENCING; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVIEW; SENSITIVITY AND SPECIFICITY; SINGLE NUCLEOTIDE POLYMORPHISM;

COLD TEMPERATURE; DNA; HUMANS; MUTATION; NUCLEIC ACID DENATURATION; PATHOLOGY, MOLECULAR; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TUMOR SUPPRESSOR PROTEIN P53;

EID: 79952940017     PISSN: 14737159     EISSN: 17448352     Source Type: Journal    
DOI: 10.1586/erm.10.115     Document Type: Review

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