Optimization of heterologous protein production in Chinese hamster ovary cells under overexpression of spliced form of human X-box binding protein

BMC Biotechnology

Volumn 14, Issue , 2014, Pages

  Gulis, Galina ^a   Simi, Kelly Cristina R ^a   de Toledo, Renata R ^a   Maranhao, Andrea Q ^a   Brigido, Marcelo M ^a  

^a UNIVERSITY OF BRASILIA   (Brazil)

Author keywords

CHO cells; Doxycycline; Heterologous protein production; T REx system; X box binding protein

Indexed keywords

CELL CULTURE; CELL ENGINEERING; CELL MEMBRANES; CLOUDS; OPTIMIZATION; PRODUCTIVITY;

BINDING PROTEINS; CHINESE HAMSTER OVARY CELLS; CHO CELL; DOXYCYCLINE; ELEVATED CONCENTRATIONS; HETEROLOGOUS PROTEIN PRODUCTION; HUMAN IMMUNOGLOBULIN G; POST-TRANSLATIONAL MODIFICATIONS;

RECOMBINANT PROTEINS;

DOXYCYCLINE; HUMAN IMMUNOGLOBULIN; HUMAN IMMUNOGLOBULIN G; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; X BOX BINDING PROTEIN 1;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BODY TEMPERATURE MEASUREMENT; CELL ENGINEERING; CELL EXPANSION; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GENE EXPRESSION SYSTEM; HETEROLOGOUS EXPRESSION; HUMAN; IMMUNOGLOBULIN PRODUCTION; IMMUNOGLOBULIN STRUCTURE; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PROTEIN FOLDING; PROTEIN SECRETION; REGULATORY MECHANISM;

ANIMALS; CHO CELLS; CRICETULUS; CULTURE MEDIA; DNA-BINDING PROTEINS; DOXYCYCLINE; ENDOPLASMIC RETICULUM; HUMANS; IMMUNOGLOBULIN G; PLASMIDS; PROTEIN ISOFORMS; TEMPERATURE; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 84899491085     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-26     Document Type: Article

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