Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells

Biotechnology and Bioengineering

Volumn 91, Issue 6, 2005, Pages 722-732

  Jones, Jullian ^a   Nivitchanyong, Tarangsri ^a   Giblin, Christina ^a   Ciccarone, Valentine ^b,d   Judd, David ^b   Gorfien, Stephen ^b   Krag, Sharon S ^c   Betenbaugh, Michael J ^a  

^a Whiting School of Engineering   (United States)

^b Invitrogen Corporation   (United States)

^c JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^d MacroGenics Inc   (United States)

Author keywords

BiP; Cell culture; ER stress; Optimization; Tetracycline; Transferrin; Unfolded protein response

Indexed keywords

CELL CULTURE; CELLS; CONCENTRATION (PROCESS); GENES; OPTIMIZATION;

BIP; ER STRESS; TETRACYCLINE; TRANSFERRIN; UNFOLDED PROTEIN RESPONSE;

PROTEINS;

CHAPERONE; GLUCOSE REGULATED PROTEIN 78; RECOMBINANT PROTEIN; TETRACYCLINE; TRANSFERRIN;

ARTICLE; CELL DENSITY; CELL GROWTH; CELL STRAIN HEK293; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CYTOMEGALOVIRUS; ENDOPLASMIC RETICULUM; HETEROLOGOUS EXPRESSION; HUMAN; HUMAN CELL; MAMMAL CELL; PROTEIN EXPRESSION; STRESS;

ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CYTOMEGALOVIRUS; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; HUMANS; MAMMALS; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TETRACYCLINE; TIME FACTORS; TRANS-ACTIVATORS; TRANSFERRIN;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; MAMMALIA;

EID: 24644516989     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20566     Document Type: Article

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