Improved HF183 quantitative real-time PCR assay for characterization of human fecal pollution in ambient surface water samples

Applied and Environmental Microbiology

Volumn 80, Issue 10, 2014, Pages 3086-3094

  Green, Hyatt C ^a   Haugland, Richard A ^a   Varma, Manju ^a   Millen, Hana T ^b   Borchardt, Mark A ^c   Field, Katharine G ^d   Walters, William A ^e   Knight, R ^e,f   Sivaganesan, Mano ^a   Kelty, Catherine A ^a   Shanks, Orin C ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b WISCONSIN WATER SCIENCE CENTER   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

^d OREGON STATE UNIVERSITY   (United States)

^e UNIVERSITY OF COLORADO   (United States)

^f HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; BIOMARKERS; RNA; SEWAGE; SURFACE WATERS;

16S RRNA GENE SEQUENCE; ACCURATE ESTIMATION; AMPLIFICATION PRODUCTS; ANALYTICAL PRECISION; HUMAN FECAL POLLUTION; INTERNAL AMPLIFICATION; LIMITS OF DETECTION; QUANTITATIVE REAL TIME PCR;

POLYMERASE CHAIN REACTION;

BIOASSAY; CONCENTRATION (COMPOSITION); DATABASE; FECAL COLIFORM; GENE EXPRESSION; GENETIC MARKER; INHIBITION; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; WATER POLLUTION;

SEWAGE;

BACTERIUM; CLASSIFICATION; FECES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SEWAGE; STANDARDS; WATER POLLUTION;

BACTERIA; FECES; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; SEWAGE; WATER MICROBIOLOGY; WATER POLLUTION;

EID: 84899050704     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.04137-13     Document Type: Article

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