Quantitative identification of fecal water pollution sources by TaqMan real-time PCR assays using Bacteroidales 16S rRNA genetic markers

Applied Microbiology and Biotechnology

Volumn 88, Issue 6, 2010, Pages 1373-1383

  Lee, Dae Young ^a   Weir, Susan C ^b   Lee, Hung ^a   Trevors, Jack T ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b NONE

Author keywords

16S rRNA genetic marker; Bacteroidales; Fecal source tracking; TaqMan real time PCR; Water pollution

Indexed keywords

16S RRNA; 16S RRNA GENE; BACTEROIDALES; CROSS REACTION; DETECTION SENSITIVITY; DOWNSTREAM AREAS; ENVIRONMENTAL WATER SAMPLES; FECAL POLLUTION; FECAL SAMPLES; FECAL SOURCES; FRESHWATER STREAMS; GENETIC MARKERS; MICROBIAL SOURCES; POLLUTION SOURCES; QUANTITATIVE IDENTIFICATION; REAL-TIME PCR; REAL-TIME PCR ASSAY; TRACKING CAPABILITY;

GENES; OIL SPILLS; POLLUTION DETECTION; POLYMERASE CHAIN REACTION; RNA; WATER POLLUTION; WATER QUALITY;

ASSAYS;

RNA 16S;

ACCURACY ASSESSMENT; ASSAY; BACTERIUM; CELL ORGANELLE; DETECTION METHOD; FECES; FRESHWATER ENVIRONMENT; GENE EXPRESSION; GENETIC MARKER; IDENTIFICATION METHOD; MAMMAL; POLLUTANT SOURCE; QUANTITATIVE ANALYSIS; RNA; SENSITIVITY ANALYSIS; WATER POLLUTION;

ARTICLE; BACBOVINE ASSAY; BACGENERAL ASSAY; BACHUMAN ASSAY; BACTEROIDALES; CONTROLLED STUDY; FALSE POSITIVE RESULT; FECES ANALYSIS; GENETIC ANALYSIS; GENETIC MARKER; HUMAN; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOURCE SINK RELATIONSHIP; WATER POLLUTION; WATER QUALITY;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; BACTEROIDETES; DNA, BACTERIAL; DNA, RIBOSOMAL; FALSE POSITIVE REACTIONS; FECES; HUMANS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; WATER MICROBIOLOGY; WATER POLLUTION;

ANSER; BACTEROIDALES; BOS; BOVINAE; CANIS FAMILIARIS; CERVIDAE; EQUIDAE; PROCYON; SUIDAE;

EID: 78649323310     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2880-0     Document Type: Article

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