Pesticide residues in honeybees, honey and bee pollen by LC-MS/MS screening: Reported death incidents in honeybees

Science of the Total Environment

Volumn 485-486, Issue 1, 2014, Pages 633-642

  Kasiotis, Konstantinos M ^a   Anagnostopoulos, Chris ^a   Anastasiadou, Pelagia ^a   Machera, Kyriaki ^a  

^a BENAKI PHYTOPATHOLOGICAL INSTITUTE   (Greece)

Author keywords

Bee pollen; Honey; Honeybee; LC MS MS; Multiresidue method; Pesticides

Indexed keywords

CHEMICAL ANALYSIS; PESTICIDES;

BEE POLLEN; HONEY; HONEYBEE; LC-MS/MS; MULTIRESIDUE METHOD;

FOOD PRODUCTS;

BEE POLLEN; HONEY; HONEYBEE; LC-MS/MS; MULTIRESIDUE METHOD; PESTICIDES;

ANIMALS; BEES; CHROMATOGRAPHY, LIQUID; ENVIRONMENTAL MONITORING; ENVIRONMENTAL POLLUTANTS; GREECE; HONEY; PESTICIDE RESIDUES; POLLEN; TANDEM MASS SPECTROMETRY;

EID: 84898877917     PISSN: 00489697     EISSN: 18791026     Source Type: Journal    
DOI: 10.1016/j.scitotenv.2014.03.042     Document Type: Article

References (61)

1. Anastassiades M., Lehotay S.J., Stajnbaher D., Schenck F.J. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. J AOAC Int 2003, 86:412-431.
2. Antoniou V., Zantopoulos N., Tsoukali H. Fatal animal poisonings in northern Greece: 1990-1995. Vet Hum Toxicol 1997, 39:35-36.
3. Bacandritsos N., Granato A., Budge G., Papanastasiou I., Roinioti E., Caldon M., et al. Sudden deaths and colony population decline in Greek honey bee colonies. J Invertebr Pathol 2010, 105:335-340.
4. Balayannis P.G. Gas chromatographic determination of coumaphos and tau-fluvalinate residues in royal jelly produced under commercial conditions. J Apic Res 2001, 40:71-78.
5. Balayiannis G., Balayiannis P. Bee honey as an environmental bioindicator of pesticides' occurrence in six agricultural areas of Greece. Arch Environ Contam Toxicol 2008, 55:462-470.
6. Blasco C., Vazquez-Roig P., Onghena M., Masia A., Pico Y. Analysis of insecticides in honey by liquid chromatography-ion trap-mass spectrometry: comparison of different extraction procedures. J Chromatogr A 2011, 1218:4892-4901.
7. Bromenshenk J.J., Preston E.M. Public-participation in environmental monitoring - A means of attaining network capability. Environ Monit Assess 1986, 6:35-47.
8. Burkle L.A., Marlin J.C., Knight T.M. Plant-pollinator interactions over 120years: loss of species, co-occurrence, and function. Science 2013, 339:1611-1615.
9. Chauzat M.P., Faucon J.P. Pesticide residues in beeswax samples collected from honey bee colonies (Apis mellifera l.) in France. Pest Manag Sci 2007, 63:1100-1106.
10. Chauzat M.P., Faucon J.P., Martel A.C., Lachaize J., Cougoule N., Aubert M. A survey of pesticide residues in pollen loads collected by honey bees in France. J Econ Entomol 2006, 99:253-262.
11. Chauzat M.P., Martel A.C., Cougoule N., Porta P., Lachaize J., Zeggane S., et al. An assessment of honeybee colony matrices, Apis mellifera (Hymenoptera Apidae) to monitor pesticide presence in continental France. Environ Toxicol Chem 2011, 30:103-111.
12. Clothianidin E. EFSA J 2013, 11. 10.2903/j.efsa.2013.3066.
13. Elzen P.J., Baxter J.R., Spivak M., Wilson W.T. Control of Varroa jacobsoni Oud. resistant to fluvalinate and amitraz using coumaphos. Apidologie 2000, 31:437-441.
14. Fidente P., Seccia S., Vanni F., Morrica P. Analysis of nicotinoid insecticides residues in honey by solid matrix partition clean-up and liquid chromatography-electrospray mass spectrometry. J Chromatogr A 2005, 1094:175-178.
15. Garcia-Chao M., Agruna M.J., Flores Calvete G., Sakkas V., Llompart M., Dagnac T. Validation of an off line solid phase extraction liquid chromatography-tandem mass spectrometry method for the determination of systemic insecticide residues in honey and pollen samples collected in apiaries from NW Spain. Anal Chim Acta; 2010, 672:107-113.
16. Girolami V., Mazzon L., Squartini A., Mori N., Marzaro M., Di Bernardo A., et al. Translocation of neonicotinoid insecticides from coated seeds to seedling guttation drops: a novel way of intoxication for bees. J Econ Entomol 2009, 102:1808-1815.
17. Giroud B., Vauchez A., Vulliet E., Wiest L., Bulete A. Trace level determination of pyrethroid and neonicotinoid insecticides in beebread using acetonitrile-based extraction followed by analysis with ultra-high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr A 2013, 10.1016/j.chroma.2013.09.088.
18. Greatti M., Sabatini A., Barbattini R., Rossi S., Stravisi A. Risk of environmental contamination by the active ingredient imidacloprid used for corn seed dressing. Preliminary results. Bull Insectology 2003, 56:69-72.
19. Gregorc A., Evans J.D., Scharf M., Ellis J.D. Gene expression in honey bee (Apis mellifera) larvae exposed to pesticides and Varroa mites (Varroa destructor). J Insect Physiol 2012, 58:1042-1049.
20. Harizanis P.C., Alissandrakis E., Tarantilis P.A., Polissiou M. Solid-phase microextraction/gas-chromatographic/mass spectrometric analysis of p-dichlorobenzene and naphthalene in honey. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2008, 25:1272-1277. [http://sitem.herts.ac.uk/aeru/iupac/].
21. ICH Validation of analytical procedures: text and methodology Q2(R1) http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf.
22. Imidacloprid E. EFSA J 2013, 11. [55 pp.]. 10.2903/j.efsa.2013.3068.
23. Johnson R.M., Ellis M.D., Mullin C.A., Frazier M. Pesticides and honey bee toxicity - USA. Apidologie 2010, 41:312-331.
24. Jovanov P., Guzsvany V., Franko M., Lazic S., Sakac M., Saric B., et al. Multi-residue method for determination of selected neonicotinoid insecticides in honey using optimized dispersive liquid-liquid microextraction combined with liquid chromatography-tandem mass spectrometry. Talanta 2013, 111:125-133.
25. Kamel A. Refined methodology for the determination of neonicotinoid pesticides and their metabolites in honey bees and bee products by liquid chromatography-tandem mass spectrometry (LC-MS/MS). J Agric Food Chem 2010, 58:5926-5931.
26. Karazafiris E., Menkissoglu-Spiroudi U., Thrasyvoulou A. New multiresidue method using solid-phase extraction and gas chromatography-micro-electron-capture detection for pesticide residues analysis in royal jelly. J Chromatogr A 2008, 1209:17-21.
27. Krupke C.H., Hunt G.J., Eitzer B.D., Andino G., Given K. Multiple routes of pesticide exposure for honey bees living near agricultural fields. PloS One 2012, 7:e29268.
28. LD50-Clothianidin European Commission, review report for the active substance clothianidin, SANCO/10533/05 - final January 2005.
29. LD50-Fipronil Peer review of the pesticide risk assessment for bees for the active substance fipronil. EFSA J 2013, 11:3158.
30. LD50-Imidacloprid http://www.bee-quick.com/reprints/imd/BayerFAQ.pdf.
31. LD50-Thiamethoxam European Commission, review report for the active substance thiamethoxam, SANCO/10390/2002 - rev. final July 2006.
32. Lozowicka B. The development, validation and application of a GC-dual detector (NPD-ECD) multi-pesticide residue method for monitoring bee poisoning incidents. Ecotoxicol Environ Saf 2013, 10.1016/j.ecoenv.2013.07.010.
33. Martel A.C., Lair C. Validation of a highly sensitive method for the determination of neonicotinoid insecticides residues in honeybees by liquid chromatography with electrospray tandem mass spectrometry. Int J Environ Anal Chem 2011, 91:978-988.
34. Menkissoglu-Spiroudi U., Tsigouri A.D., Diamantidis G.C., Thrasyvoulou A.T. Residues in honey and beeswax caused by beekeeping treatments. Fresenius Environ Bull 2001, 10:445-450.
35. Mizell R. Many plants have extrafloral nectaries helpful to beneficials. Consulté le 08 01, 2010, sur EDIS 2009, University of Florida IFAS Extension, [http://edis.ifas.ufl.edu].
36. Mullin C.A., Frazier M., Frazier J.L., Ashcraft S., Simonds R., Vanengelsdorp D., et al. High levels of miticides and agrochemicals in North American apiaries: implications for honey bee health. PLoS One 2010, 5:e9754.
37. Norgaard K.B., Cedergreen N. Pesticide cocktails can interact synergistically on aquatic crustaceans. Environ Sci Pollut Res Int 2010, 17:957-967.
38. OPERA Bee health group: bee health in Europe - facts & figs 2013.
39. Pettis J.S., Delaplane K.S. Coordinated responses to honey bee decline in the USA. Apidologie 2010, 41:256-263.
40. Pettis J.S., vanEngelsdorp D., Johnson J., Dively G. Pesticide exposure in honey bees results in increased levels of the gut pathogen Nosema. Naturwissenschaften 2012, 99:153-158.
41. Pirard C., Widart J., Nguyen B.K., Deleuze C., Heudt L., Haubruge E., et al. Development and validation of a multi-residue method for pesticide determination in honey using on-column liquid-liquid extraction and liquid chromatography-tandem mass spectrometry. J Chromatogr A 2007, 1152:116-123.
42. Porrini C., Sabatini A.G., Girotti S., Ghini S., Medrzycki P., Grillenzoni F. Honey bees and bee products as monitors of the environmental contamination. Apiacta 2003, 38:63-70.
43. Przybylski C., Segard C. Method for routine screening of pesticides and metabolites in meat based baby-food using extraction and gas chromatography-mass spectrometry. J Sep Sci 2009, 32:1858-1867.
44. Raeymaekers B. A prospective biomonitoring campaign with honey bees in a district of upper-Bavaria (Germany). Environ Monit Assess 2006, 116:233-243.
45. Reetz J.E., Zuhlke S., Spiteller M., Wallner K. Neonicotinoid insecticides translocated in guttated droplets of seed-treated maize and wheat: a threat to honeybees?. Apidologie 2011, 42:596-606.
46. SANCO Method validation and quality control procedures for pesticide residues analysis in food and feed Document No. SANCO/12495/2011 http://www.crl-pesticides.eu/library/docs/fv/SANCO12495-2011.pdf.
47. Schmuck R., Stadler T., Schmidt H.W. Field relevance of a synergistic effect observed in the laboratory between an EBI fungicide and a chloronicotinyl insecticide in the honeybee (Apis mellifera L, Hymenoptera). Pest Manag Sci 2003, 59:279-286.
48. Schneider C.W., Tautz J., Grunewald B., Fuchs S. RFID tracking of sublethal effects of two neonicotinoid insecticides on the foraging behavior of Apis mellifera. PLoS One 2012, 7:e30023.
49. Schoning R., Schmuck R. Analytical determination of imidacloprid and relevant metabolite residues by LC MS/MS. Bull Insectology 2003, 56:41-50.
50. Tanner G., Czerwenka C. LC-MS/MS analysis of neonicotinoid insecticides in honey: methodology and residue findings in Austrian honeys. J Agric Food Chem 2011, 59:12271-12277.
51. Thiamethoxam E. EFSA J 2013, 11. [68 pp.]. 10.2903/j.efsa.2013.3067.
52. Tylianakis J.M. Ecology. The global plight of pollinators. Science 2013, 339:1532-1533.
53. Tylianakis J.M. Pollination decline in context-response. Science 2013, 340:924-925.
54. Validation Bioanalytical method validation "Guidance for Industry" http://www.fda.gov/downloads/Drugs/Guidances/ucm070107.pdf.
55. Van Dijk T. Effects of neonicotinoid pesticide pollution of Dutch surface water on non-target species abundance 2010, [MSc Thesis], Utrecht University.
56. Walorczyk S., Gnusowski B. Development and validation of a multi-residue method for the determination of pesticides in honeybees using acetonitrile-based extraction and gas chromatography-tandem quadrupole mass spectrometry. J Chromatogr A 2009, 1216:6522-6531.
57. Wiest L., Bulete A., Giroud B., Fratta C., Amic S., Lambert O., et al. Multi-residue analysis of 80 environmental contaminants in honeys, honeybees and pollens by one extraction procedure followed by liquid and gas chromatography coupled with mass spectrometric detection. J Chromatogr A 2011, 1218:5743-5756.
58. Williamson S.M., Wright G.A. Exposure to multiple cholinergic pesticides impairs olfactory learning and memory in honeybees. J Exp Biol 2013, 216:1799-1807.
59. Yanez K.P., Bernal J.L., Nozal M.J., Martin M.T., Bernal J. Fast determination of imidacloprid in beeswax by liquid chromatography coupled to electrospray-mass spectrometry. Curr Anal Chem 2013, 9:495-503.
60. Yanez K.P., Martin M.T., Bernal J.L., Nozal M.J., Bernal J. Trace analysis of seven neonicotinoid insecticides in bee pollen by solid-liquid extraction and liquid chromatography coupled to electrospray ionization mass spectrometry. Food Anal Methods 2013, 10.1007/s12161-013-9710-9.
61. Zacharis C.K., Rotsias I., Zachariadis P.G., Zotos A. Dispersive liquid-liquid microextraction for the determination of organochlorine pesticides residues in honey by gas chromatography-electron capture and ion trap mass spectrometric detection. Food Chem 2012, 134:1665-1672.