Primary neuronal-astrocytic co-culture platform for neurotoxicity assessment of di-(2-ethylhexyl) phthalate

Journal of Environmental Sciences (China)

Volumn 26, Issue 5, 2014, Pages 1145-1153

  Wu, Yang ^a,b   Li, Ke ^c   Zuo, Haoxiao ^a   Yuan, Ye ^a   Sun, Yi ^b   Yang, Xu ^a  

^a CENTRAL CHINA NORMAL UNIVERSITY   (China)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Wuhan Institute for Food and Drug Control   (China)

Author keywords

Astrocyte gliosis; Di (2 ethylhexyl)phthalate; Immunostaining; Neurite length; Neuron count; Neurotoxicity; Oxidative stress

Indexed keywords

CHLORINE COMPOUNDS; ESTERS; OXIDATIVE STRESS; POLYVINYL CHLORIDES; POTASSIUM COMPOUNDS;

ASTROCYTE GLIOSIS; DI-2-ETHYLHEXYL PHTHALATE; IMMUNOSTAINING; NEURITES; NEUROTOXICITY;

NEURONS;

BIOMARKER; BRAIN; NERVOUS SYSTEM; PHTHALATE; POLLUTION EFFECT; POLLUTION EXPOSURE; RODENT; TOXICITY;

PHTHALIC ACID BIS(2 ETHYLHEXYL) ESTER; REACTIVE OXYGEN METABOLITE;

ANIMAL; ARTICLE; ASTROCYTE GLIOSIS; BAGG ALBINO MOUSE; CHEMISTRY; COCULTURE; CYTOLOGY; DI-(2-ETHYLHEXYL)PHTHALATE; DRUG EFFECT; GERMFREE ANIMAL; HUMAN; IMMUNOHISTOCHEMISTRY; LD 50; METHODOLOGY; MOUSE; NERVE CELL; NEURITE LENGTH; NEURON COUNT; NEUROTOXICITY; OXIDATIVE STRESS;

ASTROCYTE GLIOSIS; DI-(2-ETHYLHEXYL)PHTHALATE; IMMUNOSTAINING; NEURITE LENGTH; NEURON COUNT; NEUROTOXICITY; OXIDATIVE STRESS;

ANIMALS; COCULTURE TECHNIQUES; DIETHYLHEXYL PHTHALATE; HUMANS; LETHAL DOSE 50; MICE; MICE, INBRED BALB C; NEURONS; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; SPECIFIC PATHOGEN-FREE ORGANISMS;

EID: 84897491008     PISSN: 10010742     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1001-0742(13)60504-5     Document Type: Article

References (35)

1. Ahmed Z., Mazibrada G., Seabright R.J., Dent R.G., Berry M., Logan A. TACE induced cleavage of NgR and p75NTR in dorsal root ganglion cultures disinhibits outgrowth and promotes branching of neurites in the presence of inhibitory CNS myelin. The FASEB J. 2006, 20(11):1939-1941.
2. Air Toxics Website, EPAUS, 2013. Bis(2-ethylhexyl) phthalate (DEHP). Technology Transfer Network, Air Toxics Website.
3. Anderl J.L., Redpath S., Ball A.J. A neuronal and astrocyte co-culture assay for high content analysis of neurotoxicity. J. Visual. Exper. 2009, 5(27):1173. 10.3791/1173.
4. Andersen H.R., Nielsen J.B., Grandjean P. Toxicologic evidence of developmental neurotoxicity of environmental chemicals. Toxicology 2000, 144(1-3):121-127.
5. Banker G., Goslin K. Culturing Nerve Cells 1998, 135-152. The MIT Press, Massachusetts.
6. Bornehag C.G., Lundgren B., Weschler C.J., Sigsgaard T., Hägerhed-Engman L., Sundell J. Phthalates in indoor dust and their association with building characteristics. Environ. Health Perspec. 2005, 113(10):1399-1404.
7. Chao C.C., Hu S., Peterson P.K. Glia: the not so innocent bystanders. J. Neurovirol. 1996, 2(4):234-239.
8. Darenfed H., Dayanandan B., Zhang T., Hsieh S.H., Fournier A.E., Mandato C.A. Molecular characterization of the effects of Y-27632. Cell Motil. Cytos. 2007, 64(2):97-109.
9. Dringen R., Pfeiffer B., Hamprecht B. Synthesis of the antioxidant glutathione in neurons: supply by astrocytes of CysGly as precursor for neuronal glutathione. J. Neurosc. 1999, 19(2):562-569.
10. Ferguson K.K., Loch-Caruso R., Meeker J.D. Urinary phthalate metabolites in relation to biomarkers of inflammation and oxidative stress: NHANES 1999-2006. Environ. Res. 2011, 111(5):718-726.
11. Fournier A.E., Gould G.C., Liu B.P., Strittmatter S.M. Truncated soluble Nogo receptor binds Nogo-66 and blocks inhibition of axon growth by myelin. J. Neurosci. 2002, 22(20):8876-8883.
12. Fournier A.E., Takizawa B.T., Strittmatter S.M. Rho kinase inhibition enhances axonal regeneration in the injured CNS. J. Neurosci. 2003, 23(4):1416-1423.
13. Gao P.P., Yue Y., Zhang J.H., Cerretti D.P., Levitt P., Zhou R. Regulation of thalamic neurite outgrowth by the Eph ligand ephrin-A5: implications in the development of thalamocortical projections. Proceed. Natl. Acad. Sci. USA 1998, 95(9):5329-5334.
14. Govek E.E., Newey S.E., Van Aelst L. The role of the Rho GTPases in neuronal development. Genes Devel. 2005, 19(1):1-49.
15. Guo J., Han B., Qin L.J., Li B., You H.H., Yang J.W., et al. Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. PLoS One 2012, 7(6):e39008.
16. Hauser R., Meeker J.D., Duty S., Silva M., Calafat A.M. Altered semen quality in relation to urinary concentrations of phthalate monoester and oxidative metabolites. Epidemiology 2006, 17(6):682-691.
17. Hauser R., Meeker J.D., Singh N.P., Silva M.J., Ryan L., Duty S., et al. DNA damage in human sperm is related to urinary levels of phthalate monoester and oxidative metabolites. Hum. Reprod. 2007, 22(3):688-695.
18. Heudorf U., Volker Mersch-Sundermann V., Jurgen Angerer J. Phthalates toxicology and exposure. Inter. J. Hyg. Environ. Health 2007, 210(5):623-634.
19. Hsieh S.H., Ferraro G.B., Fournier A.E. Myelin-associated inhibitors regulate cofilin phosphorylation and neuronal inhibition through LIM kinase and Slingshot phosphatase. J. Neurosci. 2006, 26(3):1006-1015.
20. Koprivica V., Cho K.S., Park J.B., Yiu G., Atwal J., Gore B. EGFR activation mediates inhibition of axon regeneration by myelin and chondroitin sulfate proteoglycans. Science 2005, 310(5745):106-110.
21. Testa M.P., Alvarado O., Wournell A., Lee J., Guilford F.T., Henriksen S.H. Screening assay for oxidative stress in a feline astrocyte cell line, G355-5. J. Visual. Exper. 2011, 13:53. 10.3791/2841.
22. Meeker J.D. Exposure to environmental endocrine disruption compounds and men's health. Maturitas 2010, 66(3):236-241.
23. Meijering E., Jacob M., Sarria J.C., Steiner P., Hirling H., Unser M. Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images. Cytometry A 2004, 58(2):167-176.
24. Pool M., Thiemann J., Bar-Or B., Fournier A.E. NeuriteTracer: a novel imageJ plugin for automated quantification of neurite outgrowth. J. Neurosci. Methods 2008, 168(1):134-139.
25. Rhodes C., Orton T.C., Pratt I.S., Batten P.L., Bratt H., Jackson S.J., et al. Comparative pharmacokinetics and subacute toxicity of di(2-ethylhexyl) phthalate (DEHP) in rats and marmosets: extrapolation of effects in rodents to man. Environ. Health Perspect. 1986, 65:299-307.
26. Radio N.M., Mundy W.R. Developmental neurotoxicity testing in vitro: Models for assessing chemical effects on neurite outgrowth. Neurotoxicology 2008, 29(3):361-376.
27. Sakurai T., Lustig M., Nativ M., Hemperly J.J., Schlessinger J., Peles E. Induction of neurite outgrowth through contactin and Nr-CAM by extracellular regions of glial receptor tyrosine phosphatase beta. J. Cell Biol. 1997, 136(4):907-918.
28. Schmid P., Schlatter C. Excretion and metabolism of di-(2-ethylhexyl) phthalate in man. Xenobiotica 1985, 15(3):251-256.
29. Schwab M.E., Caroni P. Oligodendrocytes and CNS myelin are nonpermissive substrates for neurite growth and fibroblast spreading in vitro. J. Neurosci. 1988, 8(7):2381-2393.
30. Silva M.J., Barr D.B., Reidy J.A., Malek N.A., Hodge C.C., Caudill S.P., et al. Urinary levels of seven phthalate metabolites in the US population from the National Health and Nutrition Examination Survey (NHANES) 1999-2000. Environ. Health Perspect. 2004, 112(3):331-338.
31. Stephanie M.R., Stephen B., Steven S., Fernando L., Anthony G. Toxicological profile for di(2-ethylhexyl) phthalate 2002, Public Health Service Agency for Toxic Substances and Disease Registry, Georgia, USA.
32. Swan S.H. Environmental phthalate exposure in relation to reproductive outcomes and other health endpoints in humans. Environ. Res. 2008, 108(2):177-184.
33. Weuve J., Hauser R., Calafat A.M., Missmer S.A., Wise L.A. Association of exposure to phthalates with endometriosis and uterine leiomyomata: findings from NHANES, 1999-2004. Environ. Health Perspect. 2010, 118(6):825-832.
34. Yang G.T., Qiao Y.K., Li B., Yang J.W., Liu D.D., Yao H.C., et al. Adjuvant effect of di-(2-ethylhexyl) phthalate on asthmalike pathological changes in ovalbumin-immunised rats. Food Agr. Immunol. 2008, 19(4):351-362.
35. Yang L., Milutinovic P.S., Brosnan R.J., Eger E.I., Sonner J. The plasticizer di(2-ethylhexyl) phthalate modulates gamma-aminobutyric acid type A and glycine receptor function. Anesth Analg 2007, 105(2):393-396.