Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of function

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volumn 1841, Issue 4, 2014, Pages 574-580

  Pingitore, Piero ^a,b   Pirazzi, Carlo ^b   Mancina, Rosellina M ^b,c   Motta, Benedetta M ^b,d   Indiveri, Cesare ^a   Pujia, Arturo ^c   Montalcini, Tiziana ^c   Hedfalk, Kristina ^b   Romeo, Stefano ^b,c,d  

^a UNIVERSITY OF CALABRIA   (Italy)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

^c UNIVERSITY MAGNA GRAECIA OF CATANZARO   (Italy)

^d UNIVERSITY OF MILAN   (Italy)

Author keywords

Adiponutrin (ADPN); Lysophosphatidic acid acyltransferase activity; Patatin like phospholipase domain containing 3 (PNPLA3); Pichia pastoris; rs738409 (I148M); Triglyceride hydrolase activity

Indexed keywords

ACYLTRANSFERASE; LYSOPHOSPHATIDIC ACID; MEMBRANE PROTEIN; MUTANT PROTEIN; NICKEL; RECOMBINANT PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING 3 PROTEIN; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; ADIPONUTRIN, HUMAN; HYDROLASE; TRIACYLGLYCEROL;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOREACTOR; ENZYME ACTIVITY; EXPRESSION VECTOR; HUMAN; ISOTOPE LABELING; LOSS OF FUNCTION MUTATION; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; WILD TYPE; YEAST CELL; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; LIVER; METABOLISM; MOLECULAR CLONING; MUTATION; PATHOLOGY; PICHIA;

CLONING, MOLECULAR; HUMANS; HYDROLASES; LIPASE; LIVER; MEMBRANE PROTEINS; MUTATION; PICHIA; RECOMBINANT PROTEINS; TRIGLYCERIDES;

EID: 84896895656     PISSN: 13881981     EISSN: 18792618     Source Type: Journal    
DOI: 10.1016/j.bbalip.2013.12.006     Document Type: Article

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