Enhanced stabilization of a stable single domain antibody for SEB toxin by random mutagenesis and stringent selection

Protein Engineering, Design and Selection

Volumn 27, Issue 3, 2014, Pages 89-95

  Turner, Kendrick B ^a   Zabetakis, Dan ^a   Goldman, Ellen R ^a   Anderson, George P ^a  

^a NAVAL RESEARCH LABORATORY   (United States)

Author keywords

Differential scanning calorimetry; Phage display; Random mutagenesis; Single domain antibody; Thermal stabilization

Indexed keywords

CHEMICAL DENATURATION; DEGREE OF STABILITY; HIGHER MELTING POINTS; PHAGE DISPLAY; RANDOM MUTAGENESIS; SINGLE-DOMAIN ANTIBODIES; STAPHYLOCOCCAL ENTEROTOXIN B; THERMAL STABILIZATION;

DIFFERENTIAL SCANNING CALORIMETRY; MELTING POINT; MUTAGENESIS; STABILIZATION;

ANTIBODIES;

NANOBODY; STAPHYLOCOCCUS ENTEROTOXIN B;

ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; BIOPANNING; DISULFIDE BOND; HIGH TEMPERATURE; MELTING POINT; MOLECULAR STABILITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN STRUCTURE; THERMOSTABILITY;

DIFFERENTIAL SCANNING CALORIMETRY; PHAGE DISPLAY; RANDOM MUTAGENESIS; SINGLE DOMAIN ANTIBODY; THERMAL STABILIZATION;

AMINO ACID SEQUENCE; ANIMALS; CAMELIDS, NEW WORLD; ENTEROTOXINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN ENGINEERING; PROTEIN STABILITY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SINGLE-DOMAIN ANTIBODIES; TEMPERATURE;

EID: 84896689246     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzu001     Document Type: Article

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