Single-domain antibody fragments with high conformational stability

Protein Science

Volumn 11, Issue 3, 2002, Pages 500-515

  Dumoulin, Mireille ^a   Conrath, Katja ^b   Van Meirhaeghe, Annemie ^b   Meersman, Filip ^c   Heremans, Karel ^c   Frenken, Leon G J ^d   Muyldermans, Serge ^b   Wyns, Lode ^b   Matagne, Andre ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^c UNIVERSITY OF LEUVEN   (Belgium)

^d UNILEVER   (Netherlands)

Author keywords

Camel heavy chain antibodies; Circular dichroism; Fluorescence; Fourier transform infrared spectroscopy; High pressure; Protein folding; Protein stability; Surface plasmon resonance

Indexed keywords

ANTIBODY;

ANTIBODY SPECIFICITY; ANTIGEN RECOGNITION; ARTICLE; CIRCULAR DICHROISM; DENATURATION; INFRARED SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STABILITY; RENATURATION; SOLUBILITY; SURFACE PLASMON RESONANCE; THERMODYNAMICS;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BETA-LACTAMASES; CAMELIDS, NEW WORLD; CAMELS; HEAT; HUMANS; IMMUNOGLOBULIN FRAGMENTS; MOLECULAR SEQUENCE DATA; MURAMIDASE; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; SPECTROMETRY, FLUORESCENCE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

CAMELIDAE;

EID: 0036182644     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.34602     Document Type: Article

References (89)

1. Refinement of human lysozyme at 1.5 Å resolution
2. Escherichia coli secretion of an active chimeric antibody fragment
3. Single-chain antigen-binding proteins
4. Crystal structure of lysozyme by X-ray diffraction
5. Examination of the secondary structure of proteins by deconvolved FTIR spectra
6. A melanoma-specific VH antibody cloned from a fusion phage library of a vaccinated melanoma patient
7. The 3-D structure of a zinc metallo-β-lactamase from Bacillus cereus reveals a new type of protein fold
8. Inhibition par des polymères de la N-acetylglucosamine de l'activitè lysante à pH 6,2 de lysozymes d'origines diffèrentes vis-à-vis de Micrococcus kysodeikiicus
9. β-lactamase inhibitors derived from single-domain antibody fragments elicited in Camelidae
10. "Camelising" human antibody fragments: NMR studies on VH domains
11. Single antibody domains as small recognition units: Design and in vitro antigen selection of camelized, human VH domains with improved protein stability
12. A single-domain antibody fragment in complex with RNase A: Non-canonical loop structures and nanomolar affinity using two CDR loops
13. Canonical antigen-binding loop structures in immunoglobulins: More structures, more canonical classes?
14. Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme
15. Contribution of the carbohydrate moiety to conformational stability of the carboxypeptidase Y. High pressure study
16. Reversible dissociation and conformational stability of dimeric ribulose bisphosphate carboxylase
17. Protein stability
18. Isolation of antigen specific llama VH antibody fragments and their high level secretion by Saccharomyces cerevisiae
19. Selection and identification of single domain antibody fragments from camel heavy-chain antibodies
20. Clinical trials of antibody therapy
21. Monoclonal antibody therapy for solid tumors
22. Naturally occurring antibodies devoid of light chains
23. Does Fourier-transform infrared-spectroscopy provide useful information on protein structures
24. The conformational, analysis of peptides using Fourier transform IR spectroscopy
25. Secretion of functional antibody and Fab fragment from yeast cells
26. Recombinant antibody fragments
27. Tertiary interactions in the folding pathway of hen lysozyme: Kinetic studies using fluorescent probes
28. The use and misuse of FTIR spectroscopy in the determination of protein structure
29. Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting
30. Sequence of proteins of immunological interest
31. Fourier self-deconvolution - A method for resolving intrinsically overlapped bands
32. The molten globule state as a clue for understanding the folding and cooperativity of globular-protein structure
33. Stopped-flow circular dichroism
34. Potent enzyme inhibitors derived from dromedary heavy-chain antibodies
35. P22 Arc repressor: Enhanced expression of unstable mutants by addition of polar C-terminal sequences
36. Single-domain camel antibodies: Current status
37. Unique single-domain binding fragments derived from naturally occuring camel heavy-chain antibodies
38. Sequence and structure of VH domain from naturally occurring camel heavy chain immunoglobulins lacking light chains
39. Recognition of antigens by single-domain antibody fragments: The superfluous luxury of paired domains
40. Denaturant m values and heat capacity changes: Relation to changes in accessible surface areas of protein unfolding
41. Recovery of antigens from immunoadsorbents using high pressure
42. Determination and analysis of urea and guanidine hydrochloride denaturation curves
43. Measuring and increasing protein stability
44. Conformational stability of globular proteins
45. Structural characterization of the pressure-unfolded state of staphylococcal nuclease by synchrotron small-angle X-ray scattering and Fourier-transform infrared spectroscopy
46. Thermal unfolding of a llama antibody fragment: A two-state reversible process
47. Optimal (Wiener) filtering with the FFT
48. Natural antibody missing a cysteine in VH: Consequences for thermodynamic stability and folding
49. Evidence for a molten globule state as a general intermediate in protein folding
50. TEM β-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis
51. Stability and folding properties of a model β-sheet protein, Escherichia coli CspA
52. Engineering antibody Fv fragments for cancer detection and therapy: Disulfide-stabilized Fv fragments
53. Single domain antibodies: Comparison of camel VH and camelised human VH domains
54. Expression of an antibody Fv fragment in myeloma cells
55. Fluorescence spectroscopy
56. High pressure study of the liquid/solid interface: Surface enhanced raman scattering from adsorbed molecules
57. Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethane-sulfonyl alpha-chymotrypsin using different denaturants
58. Extremely rapid protein folding in the absence of intermediates
59. Optical spectroscopy to characterize protein conformation and conformational changes
60. Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe
61. Comparative thermodynamic of the Fv, Fab* and Fab fragments of anti-dansyl mouse monoclonal antibody
62. Pressure dissociation and conformational drift of the beta dimer of tryptophan synthetase
63. Dissociation of a native dimer to a molten globule monomer. Effects of pressure and dilution on the association equilibrium of arc repressor
64. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli
65. Determination of the secondary structure of proteins at high pressure
66. How to minimize certain artifacts in Fourier self-deconvolution
67. Disaggregation of Alzheimer beta-amyloid by site-directed mAb
68. Monoclonal antibodies inhibit in vitro fibrillar aggregation of the Alzheimer beta-amyloid peptide
69. The crystal structure of a llama heavy chain variable domain
70. Camelid heavy-chain variable domains provide efficient combining sites to haptens
71. Pressure-induced dissociation of antigen-antibody complexes
72. X-ray analysis of the NMC-A β-lactamase at 1.64-Å resolution, a class A carbapenemase with broad substrate specificity
73. Pressure versus temperature unfolding of ribonuclease A: An FTIR spectroscopic characterization of 10 variants at the carboxy-terminal site
74. Camel single-domain antibody inhibits enzyme by mimicking carbohydrate substrate
75. Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies
76. Induction of immune responses and molecular cloning of the heavy chain antibody repertoire of Lama glama
77. Determination of protein secondary structure
78. Comparison of llama VH sequences from conventional and heavy chain antibodies
79. Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
80. Conformation, pH-induced conformational changes and thermal unfolding of anti-p25 (HIV-1) monoclonal antibody CB4-1 and its Fab and Fc fragments
81. Pressure effects on protein secondary structure and hydrogen deuterium exchange in chymotrypsinogen: A Fourier transform infrared spectroscopic study
82. A new internal pressure calibrant for high-pressure infrared spectroscopy of aqueous systems
83. Contributions of tryptophan side chains to the far-ultraviolet circular dichroism of proteins
84. Mutual stabilisation of VL and VH in single-chain antibody fragments, investigated with mutants engineered for stability
85. Different equilibrium stability behavior of scFv fragments: Identification, classification, and improvement by protein engineering
86. Correlation between in vitro stability and in vivo performance of anti-GCN4 intrabodies as cytoplasmic inhibitors
87. Length distribution of CDR H3 in antibodies
88. Effects of substitutions of amino acids on the thermal stability of the Fv fragments of antibodies
89. Thermal stabilisation of a single-chain Fv antibody fragment by introduction of a disulfide bond