Fast image analysis for the micronucleus assay in a fully automated high-throughput Biodosimetry system

Radiation Research

Volumn 181, Issue 2, 2014, Pages 146-161

  Lyulko, Oleksandra V ^a   Garty, Guy ^a   Randers Pehrson, Gerhard ^a   Turner, Helen C ^a   Szolc, Barbara ^a,b   Brenner, David J ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b NEW YORK UNIVERSITY COLLEGE OF DENTISTRY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ALGORITHM; ARTICLE; AUTOANALYZER; BINUCLEAR CELL; CELL NUCLEUS; CELL TYPE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOPLASM; DOSIMETRY; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMAGING SOFTWARE; IN VITRO STUDY; MICRONUCLEUS; MICRONUCLEUS TEST; MONONUCLEAR CELL; NORMAL HUMAN; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; RADIATION DOSE; RADIATION EXPOSURE; RADIATION RESPONSE; RAPID AUTOMATED BIODOSIMETRY TOOL;

AUTOMATION; DOSE-RESPONSE RELATIONSHIP, RADIATION; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LYMPHOCYTES; MICRONUCLEUS TESTS; MOLECULAR IMAGING; RADIOMETRY; SOFTWARE; TIME FACTORS;

EID: 84896298023     PISSN: 00337587     EISSN: 19385404     Source Type: Journal    
DOI: 10.1667/RR13441.1     Document Type: Article

References (53)

1. Pellmar TC, Rockwell S. Priority list of research areas for radiological nuclear threat countermeasures. Radiat Res 2005; 163:115-23.
2. Salerno A, Zhang J, Bhatla A, Lyulko OV, Dutta A, Garty G, et al. Design considerations for a minimally invasive high-throughput automation system for radiation biodosimetry. In: Automation Science and Engineering. CASE 2007: Proceedings of the 3rd IEEE International Conference on Automation Science and Engineering; 2007 Sept 22-25; Scottsdale, AZ. p. 846-52.
3. Garty G, Chen Y, Salerno A, Turner HC, Zhang J, Lyulko OV, et al. The RABiT: A rapid automated biodosimetry tool for radiological triage. Health Phys 2010; 98:209-17.
4. Garty G, Chen Y, Turner HC, Zhang J, Lyulko OV, Bertucci A, et al. The RABiT: A Rapid Automated BIodosimetry Tool For Radiological Triage II. Technological Developments. Int J Rad Biol 2011; 87:776-90.
5. Chen Y, Zhang J, Wang H, Garty G, Xu Y, Lyulko OV, et al. Design and preliminary validation of a rapid automated biodosim-etry tool for high-throughput radiological triage. MESA 2009: Proceedings of the 5th ASME/IEEE International Conference on Mechatronic and Embedded Systems and Applications; 2009 Aug 30 -Sept 2; San Diego, CA. 3; p. 61-7.
6. Garty G, Karam A, Brenner DJ. Infrastructure to support ultra high throughput biodosimetry screening after a radiological event. Int J Radiat Biol 2011; 87:754-65.
7. Nakamura A, Sedelnikova OA, Redon C, Pilch DR, Sinogeeva NI, Shroff R, et al. Techniques for gamma-H2AX detection. Meth Enzymol 2006; 409:266-50.
8. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998; 273:5858-68.
9. Fenech M. Cytokinesis-block micronucleus cytome assay. Nat Protoc 2007; 2:1084-104.
10. Turner HC, Brenner DJ, Chen Y, Bertucci A, Zhang J, Wang H, et al. Adapting the c-H2AX assay for automated processing in human lymphocytes. 1. Technological aspects. Radiat Res 2011; 175:282-90.
11. Gough AH, Johnston PA. Requirements, features, and performance of high content screening platforms. Methods Mol Biol 2007; 656:41-61.
12. Pepperkok R, Ellenberg J. High-throughput fluorescence microscopy for systems biology. Nat Rev Mol Cell Biol 2006; 7:690-6.
13. Fenech M, Morley AA. Measurement of micronuclei in lymphocytes. Mutat Res 1985; 147:29-36.
14. Heddle JA. A rapid in vivo test for chromosomal damage. Mutat Res 1973; 18:187-90.
15. Schmid W. Chemical mutagen testing on in vivo somatic mammalian cells. Agents Actions 1973; 3:77-85.
16. Kirsch-Volders M. Towards a validation of the micronucleus test. Mutat Res 1997; 392:1-4.
17. International Atomic Energy Agency. Cytogenetic analysis for radiation dose assessment: a manual. Vienna: IAEA; 2001.
18. Amundson SA, Bittner M, Meltzer P, Trent J, Fornace AJ Jr. Biological indicators for the identification of ionizing radiation exposure in humans. Expert Rev Mol Diagn 2001; 1:211-9.
19. Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res 2003; 534:65-75.
20. Iarmarcovai G, Bonassi S, Botta A, Baan RA, Orsière T. Genetic polymorphisms and micronucleus formation: A review of the literature. Mutat Res 2008; 658:215-33.
21. Fenech M, Holland N, Zeiger E, Chang WP, Burgaz S, Thomas P, et al. The HUMN and HUMNxL international collaboration projects on human micronucleus assays in lymphocytes and buccal cells-past, present and future. Mutagenesis 2011; 26:239-45.
22. Fenech M, Jarvis LR, Morley AA. Preliminary studies on scoring micronuclei by computerised image analysis. Mutat Res 1988; 203:33-8.
23. Böcker W, Mü ller W-U, Streffer C. Image processing algorithms for the automated micronucleus assay in binucleated human lymphocytes. Cytometry 1995; 19:283-94.
24. Varga D, Johannes T, Jainta S, Schuster S, Schwarz-Boeger U, Kiechle M, et al. An automated scoring procedure for the micronucleus test by image analysis. Mutagenesis 2004; 19:391-7.
25. Böcker W, Streffer C, Mü ller W-U, Yu C. Automated scoring of micronuclei in binucleated human lymphocytes. Int J Radiat Biol 1996; 70:529-37.
26. Castelain P, Van Hummelen P, Deleener A, Kirsch-Volders M. Automated detection of cytochalasin-B blocked binucleated lymphocytes for scoring micronuclei. Mutagenesis 1993; 8:285-93.
27. Decordier I, Papine A, Plas G, Roesems S, Vande Loock K, Moreno-Palomo J, et al. Automated image analysis of cytokinesisblocked micronuclei: an adapted protocol and a validated scoring procedure for biomonitoring. Mutagenesis 2009; 24:85-93.
28. Szirmai S, Berces J, Koteles GJ. Computerized Image Analysis for Determining Micronucleus Frequency. Environ Health Perspect 1993; 101 Suppl 3:S57-60.
29. Tates AD, Van Welie MT, Ploem JS. The present state of the automated micronucleus test for lymphocytes. Int J Radiat Biol 1990; 58:813-25.
30. Diaz D, Scott A, Carmichael P, Shi W, Costales C. Evaluation of an automated in vitro micronucleus assay in CHO-K1 cells. Mutat Res 2007; 630:1-13.
31. Donnelly EH, Nemhauser JB, Smith JM, Kazzi ZN, Farfán EB, Chang AS, et al. Acute radiation syndrome: assessment and management. South Med J 2010; 103:541-4.
32. International Atomic Energy Agency. Diagnosis and treatment of radiation injuries. Vienna: IAEA; 1998. (http://www-pub.iaea.org/MTCD/publications/PDF/ P040-scr.pdf)
33. Hall EJ, Giaccia AJ. Radiobiology for the radiologist. 6th ed. Philadelphia: Lippincott Williams &Wilkins; 2006.
34. Armed Forces Radiobiology Research Institute. Medical management of radiological casualties. Bethesda: AFFRI; 2003.
35. Baranov A, Gale RP, Guskova A, Piatkin E, Selidovkin G, Muravyova L, et al. Bone marrow transplantation after the Chernobyl nuclear accident. N Engl J Med 1989; 321:205-12.
36. Müller W-U, Rode A. The micronucleus assay in human lymphocytes after high radiation doses (5-15 Gy). Mutat Res 2002; 502:47-51.
37. Romm H, Barnard S, Boulay-Greene H, De Amicis A, De Sanctis S, Franco M, et al. Laboratory intercomparison of the cytokinesisblock micronucleus assay. Radiat Res 2013; 180:120-8.
38. Yao B, Jiang BR, Ai HS, Li YF, Liu GX, Man QH, et al. Biological dose estimation for two severely exposed patients in a radiation accident in Shandong Jining, China, in 2004. Int J Radiat Biol 2010; 86:800-8.
39. Yao B, Li Y, Liu G, Guo M, Bai J, Man Q, et al. Estimation of the biological dose received by five victims of a radiation accident using three different cytogenetic tools. Mutat Res 2013; 751:66-72.
40. Thomas P, Fenech M. Cytokinesis-block micronucleus cytome assay in lymphocytes. In: Didenko VV, editor. DNA damage detection in situ, ex vivo, and in vivo: Methods and protocols, Methods Mol Biol 2011; 682:217-34.
41. Slowinski J, Mazurek U, Bierzynska-Macyszyn G, Widel M, Latocha M, Glogowska-Ligus J, et al. Cell proliferative activity estimated by histone H2B mRNA level correlates with cytogenetic damage induced by radiation in human glioblastoma cell lines. J Neurooncol 2005; 71:237-43.
42. Wu H, Hada M, Meador J, Hu X, Rusek A, Cucinotta FA. Induction of micronuclei in human fibroblasts across the Bragg curve of energetic heavy ions. Radiat Res 2006; 166:583-9.
43. Otsu N. A threshold selection method from gray-level histograms. IEEE Trans Syst Man Cybern 1979; 9:62-6.
44. Ridler TW, Calvard S. Picture thresholding using an iterative selection method. IEEE Trans Syst Man Cybern 1978; 8:630-2.
45. McNamee JP, Flegal FN, Greene HB, Marro L, Wilkins R. Validation of the cytokinesis-block micronucleus (CBMN) assay for use as a triage biological dosimetry tool. Radiat Prot Dosim 2009; 135:232-42.
46. Fenech M. Important variables that influence base-line micronucleus frequency in cytokinesis-blocked lymphocytes-A biomarker for DNA damage in human populations. Mutat Res 1998; 404:155-65.
47. Kato TA, Wilson PF, Nagasaw H, Peng Y, Weil MM, Little JB, et al. Variations in radiosensitivity among individuals: a potential impact on risk assessment? Health Phys 2009; 97:470-80.
48. Kacprzak J, Kuszewski T, Lankoff A, Mü ller W-U, Wojcik A, Lisowska H. Individual variations in the micronucleus assay for biological dosimetry after high dose exposure. Mutat Res 2013; 756:196-200.
49. Di Giorgio C, De Meo MP, Laget M, Guiraud H, Botta A, Dumenil G. The micronucleus assay in human lymphocytes: screening for inter-individual variability and application to biomonitoring. Carcinogenesis 1994; 15:313-7.
50. Duffaud F, Orsière T, Villlani P, Pelissier AL, Volot F, Favre E, et al. Comparison between micronucleated lymphocyte rates observed in healthy subjects and cancer patients. Mutagenesis 1997; 12:227-31.
51. Swartz H, Flood AB, Gougelet RM, Rea ME, Nicolalde RJ, Williams BB. A critical assessment of biodosimetry methods for large-scale incidents. Health Phys 2010; 98:95-108.
52. Willems P, August L, Slabbert J, Romm H, Oestreicher U, Thierens H, et al. Automated micronucleus (MN) scoring for population triage in case of large scale radiation events. Int J Radiat Biol 2010; 86:2-11.
53. Dolphin GW. Biological dosimetry with particular reference to chromosome aberration analysis. A review of methods. Handling of Radiation Accidents, IAEA, Vienna 1969; 215-24.