Automated micronucleus (MN) scoring for population triage in case of large scale radiation events

International Journal of Radiation Biology

Volumn 86, Issue 1, 2010, Pages 2-11

  Willems, Petra ^a   August, Liezel ^b   Slabbert, Jacobus ^b   Romm, Horst ^c   Oestreicher, Ursula ^c   Thierens, Hubert ^a   Vral, Anne ^a  

^a GHENT UNIVERSITY   (Belgium)

^b IThemba LABS ^*  (South Africa)

^c Bundesamt fuer Strahlenschutz   (Germany)

Author keywords

Automated micronucleus (MN) assay; Biological dosimetry; Cytogenetic assay; Large scale irradiation event; Population triage

Indexed keywords

ADULT; ARTICLE; BELGIUM; BLOOD SAMPLING; CLINICAL ARTICLE; COMPUTER PROGRAM; CYTOLOGY; DIAGNOSTIC ACCURACY; DOSE RESPONSE; EMERGENCY HEALTH SERVICE; FEMALE; GERMANY; HUMAN; HUMAN CELL; LABORATORY; MALE; MICRONUCLEUS; NORMAL HUMAN; POPULATION; PRIORITY JOURNAL; RADIATION ACCIDENT; RADIATION DOSE; RADIATION EXPOSURE; REPRODUCIBILITY; SCORING SYSTEM; SENSITIVITY AND SPECIFICITY; STANDARD; UNCERTAINTY; VISION;

ADULT; DOSE-RESPONSE RELATIONSHIP, RADIATION; FEMALE; HUMANS; INDOLES; MALE; MICRONUCLEUS TESTS; MIDDLE AGED; RADIOACTIVE HAZARD RELEASE; TRIAGE;

EID: 75149176761     PISSN: 09553002     EISSN: 13623095     Source Type: Journal    
DOI: 10.3109/09553000903264481     Document Type: Article

References (34)

1. AFRRI (Armed Forces Radiobiology Research Institute). Accessed 10 October 2008 from the website: http://www.afrri. usuhs.mil/.
2. Alexander G, Swartz H, Amundson S, Blakely W, Buddemeier B, Gallez B, Dainiak N, Goans R, Hayes R, Lowry P, Noska M, Okunieff P, Salner A, Schauer D, Trompier F, Turteltaub K, Voisin P, Wiley AJ, Wilkins R. 2007. BiodosEPR-2006 Meeting: Acute dosimetry consensus committee recommendations on biodosimetry applications in events involving uses of radiation by terrorists and radiation accidents. Radiation Measurements 42:972-996.
3. Berger M, Christensen D, Lowry P, Jones O, Wiley A. 2006. Medical management of radiation injuries: Current approaches. Occupational Medicine 56:162-172.
4. Berger M, Leonard R, Ricks R, Wiley A, Lowry P, Flynn D. 2008. Hospital triage in the first 24 hours after a nuclear or radiological disaster. Accessed 10 October 2008 from the website: http://orise.orau.gov/reacts/.
5. Castelain P, Van Hummelen P, Deleener A, Kirsch-Volders M. 1993. Automated detection of cytochalasin-B blocked binucleated lymphocytes for scoring micronuclei. Mutagenesis 8:285-293.
6. Decordier I, Papine A, Plas G, Roesems S, Vande Loock K, Moreno-Palomo J, Cemeli E, Anderson D, Fucic A,Marcos R, Soussaline F, Kirsch-Volders M. 2009. Automated image analysis of cytokinesis-blocked micronuclei: An adapted protocol and a validated scoring procedure for biomonitoring. Mutagenesis 24:85-93.
7. Fenech M. 2000. The in vitro micronucleus technique. Mutation Research 455:81-95.
8. Fenech M, Chang W, Kisch-Volders M, Holland N, Bonassi S, Zeiger E, project HM. 2003. HUMN project: Detailed description of the scoring criteria for the cytokinesis-block micronusleus assay using isolated human lymphocyte cultures. Mutation Research 534:65-75.
9. Fenech M, Morley A. 1985. Measurement of micronuclei in lymphocytes. Mutation Research 147:29-36.
10. International Atomic Energy Agency (IAEA). 1987. Absorbed dose determination in photon and electron beams: An international code of practice. Technical Report Series 277. Vienna: IAEA.
11. International Atomic Energy Agency (IAEA). 1998. Diagnosis and treatement of radiation injuries. Safety Reports Series No. 2.
12. International Atomic Energy Agency (IAEA). 2001. Cytogenetic analysis for radiation dose assessment: A manual. Technical Report No. 405.
13. Kuniak M, Azizova T, Day R, Wald N, Suyama J, Zhang A, Sumina MV, Pesternikova VS, Vasilenko E, Soaita A, Slaughter DM. 2008. The Radiation Injury Severity Classification system: An early injury assessment tool for the frontline health-care provider. British Journal of Radiology 81:232-243.
14. Leonard A, Rueff J, Gerber G, Leonard E. 2005. Usefulness and limits of biological dosimetry based on cytogenetic methods. Radiation Protection Dosimetry 115:448-454.
15. Lloyd D, Edwards AA, Moquet JE, Guerrero-Carbajal YC. 2000. The role of cytogenetics in early triage of radiation casualties. Applied Radiation and Isotopes 52:1107-1112.
16. Loong T. 2003. Understanding sensitivity and specificity with the right side of the brain. British Medical Journal 327:716-719.
17. Pala FS, Alkaya F, Tabakçioglu K, Tokatli F, Uzal C, Parlar S, Algünes Ç. 2008. The effects of micronuclei with whole chromosome on biological dose estimation. Turk Biyoloji Dergisi 32:283-290.
18. Roy L, Roch-Lefevre S, Vaurijoux A, Voisin P, Martin C, Gregoire E, Voisin P. 2007. Optimization of cytogenetic procedures for population triage in case of radiological emergency. Radiation Measurements 42:1143-1146.
19. Schunck C, Johannes T, Varga D, Lörch T, Plesch A. 2004. New developments in automated cytogenetic imaging: Unattended scoring of dicentric chromosomes, micronuclei, single cell gel electrophoresis, and fluorescence signals. Cytogenetic and Genome Research 104:383-389.
20. Stephan G, Oestreicher U, Romm H. 2007. Biological dosimetry. chromosomal alterations. In: Obe G, Vijayalaxmi, editors. Heidelberg: Springer. pp 201-209.
21. Thierens H, Vral A, Barbé M, Aoulasalah B, De Ridder L. 1999a. A cytogenetic study of nuclear power plant workers using the micronucleus- centromere assay. Mutation Research 445: 105-111.
22. Thierens H, Vral A, De Ridder L. 1996. Cytogenetic study of radiological workers: Effect of age, smoking and radiation burden on the micronucleus frequency. Mutation Research 360:75-82.
23. Thierens H, Vral A, De Ridder L, Touil N, Kirsch-Volders M, Lambert V, Laurent C. 1999b. Inter-laboratory comparison of cytogenetic endpoints for the biomonitoring of radiological workers. International Journal of Radiation Biology 75:23-34.
24. Thierens H, Vral A, Morthier R, Aoulasalah B, De Ridder L. 2000. Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay. Mutagenesis 15:245-249.
25. Thomas P, Umegaki K, Fenech M. 2003. Nucleoplasmic bridges are a sensitive measure of chromosome rearrangement in the cytokinesis-block micronucleus assay. Mutagenesis 18:187-194.
26. Varga D, Johannes T, Jainta S, Schwarz-Boeger U, Kiechle M, Garcia BP, Vogel W. 2004. An automated scoring procedure for the micronucleus test by image analysis. Mutagenesis 19:391-397.
27. Verhaegen F, Vral A, Seuntjens J, Schipper N, De Ridder L, Thierens H. 1994. Scoring of radiation-induced micronuclei in cytokinesis-blocked human-lymphocytes by automated imageanalysis. Cytometry 17:119-127.
28. Voisin P, Benderitter M, Claraz M, Chambrette V, Sorokine-Durm I, Delbos M, Durand V, Leroy A, Paillole N. 2001. The cytogenetic dosimetry of recent accidental overexposure. Cellular and Molecular Biology 47:557-564.
29. Vral A, Thierens H, De Ridder L. 1997. In vitro micronucleuscentromere assay to detect radiation-damage induced by low doses in human lymphocytes. International Journal of Radiation Biology 71:61-68.
30. Vral A, Verhaegen F, Thierens H, De Ridder L. 1994a. The in vitro cytokinesis-block micronucleus assay - a detailed description of an improved slide preparation technique for the automated detection of micronuclei in human-lymphocytes. Mutagenesis 9:439-443. (Pubitemid 24297872)
31. Vral A, Verhaegen F, Thierens H, De Ridder L. 1994b. Micronuclei induced by fast neutrons versus 60Co-rays in human peripheral blood lymphocytes. International Journal of Radiation Biology 65:321-328.
32. Vral A, Verhaegen F, Thierens H, De Ridder L. 1994c. Micronuclei induced by fast neutrons versus 60Co g-rays in human peripheral blood lymphocytes. International Journal of Radiation Biology 65:321-328.
33. Wilkins R, Romm H, Kao T, Awa A, Yoshida M, Livingston G, Jenkins M, Oestreicher U, Pellmar T, Prasanna P. 2008. Interlaboratory comparison of the dicentric chromosome assay for radiation biodosimetry in mass casualty events. Radiation Research 169:551-560.
34. Wojcik A, Gregoire E, Hayata I, Roy L, Sommer S, Stephan G, Voisin P. 2004. Cytogenetic damage in lymphocytes for the purpose of dose reconstruction: A review of three recent radiation accidents. Cytogenetic and Genome Research 104:200-205.