Automated scoring of micronuclei in binucleated human lymphocytes

International Journal of Radiation Biology

Volumn 70, Issue 5, 1996, Pages 529-537

  Bocker W ^a   Streffer, C ^a   Muller W U ^a   Yu, C ^a  

^a UNIVERSITY HOSPITAL ESSEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOMATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; LYMPHOCYTE; MICRONUCLEUS TEST; PRIORITY JOURNAL; RADIATION EXPOSURE; X IRRADIATION;

EID: 0029911560     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/095530096144734     Document Type: Article

References (20)

1. BÖCKER, W., MÜLLER, W.-U., and STREFFER, C., 1993, A digital image-analysis system for the automated micronucleus assay in cytochalasin B blocked human lymphocytes. Proceedings of the 25th Annual Meeting of the European Society for Radiation Biology, Stockholm, Sweden.
2. BÖCKER, W., MÜLLER, W.-U., and STREFFER, C., 1995a, Image processing algorithms for the automated micronucleus assay in binucleated human lymphocytes. Cytometry, 19, 283-294.
3. BÖCKER, W., MÜLLER, W.-U., and STREFFER, C., 1995b, Comparison of different automatic threshold algorithms for image segmentation in microscope images. Proceedings of Applications of Digital Image Processing XVIII, SPIE San Diego, pp. 230-241.
4. CASTELAIN, P., VAN HUMMELEN, P., DELEENER, A., and KIRSCH-VOLDERS, M., 1993, Automated detected of cytochalasin-B blocked binucleated lymphocytes for scoring micronuclei. Mutagenesis, 8/4, 285-293.
5. FENECH, M., and MORLEY, A., 1985a, Solutions to the kinetic problem in the micronucleus assay, Cytobios, 43, 233-246.
6. FENECH, M. and MORLEY, A., 1985b, Measurement of micronuclei in lymphocytes. Mutation Research, 147, 29-36.
7. GANTENBERG, H. W., WUTTKE, K., STREFFER, C., and MÜLLER, W. U., 1991, Micronuclei in human lymphocytes irradiated in vitro or in vivo. Radiation Research, 128, 276-281.
8. HEDDLE, J. A., 1973, A rapid in vivo test for chromosomal damage. Mutation Research, 19, 187-190.
9. MADLE, S., NOWAK, J., and OBE, G., 1976, Effects of inhibitors of DNA, RNA and protein synthesis on frequencies and types of premature chromosome condensation from X-ray induced micronuclei. Humangenetik, 34, 143-149.
10. MOLLS, M., STREFFER, C., and ZAMBOGLOU, N., 1981, Micronucleus formation in preimplanted mouse embryos cultured in vitro after irradiation with X-rays and neutrons. International Journal of Radiation Biology, 39, 307-314.
11. MÜLLER, W. U., and STREFFER, C., 1984, Distribution of micronuclei among single cells of pre-implantation mouse embryos after X-irradiation in vitro. Mutation Research, 25, 65-70.
12. MÜLLER, W. U., and STREFFER, C., 1994, Micronucleus assays. In: Advances in Mutagenesis Research. Edited by: G. Obe (Berlin: Springer), Vol. 5, pp. 1-134.
13. OBE, G., and BEEK, B., 1975, The human leukocyte test system. VII. Further investigations concerning micronucleus-derived premature chromosome condensation. Humangenetik, 30, 143-154.
14. SCHMID, W., 1973, Chemical mutagen testing on in vivo somatic mammalian cells. Agents and Actions, 3, 77-85.
15. SCHREIBER, G. A., BEISKER, W., BRASELMANN, H., BAUGHINGER, M., BÖGL, K. W., and NÜSSE, M., 1992, An automated flow cytometric micronucleus assay for human lymphocytes. International Journal of Radiation Biology, 62, 695-709.
16. SERRA, J., 1982, Image Analysis and Mathematical Morphology (New York: Academic Press).
17. TATES, A. D., VAN WELIE, M. T., and PLOEM, J. S., 1990, The present state of the automated micronucleus test for lymphocytes. International Journal of Radiation Biology, 58, 813-825.
18. VERHAEGEN, F., VRAL, A., and SCHIPPER, N. W., 1993, Automated scoring of micronuclei in Cytochalasin-B blocked binucleated human lymphocytes. Proceedings of the 25th Annual Meeting of the European Society for Radiation Biology, Stockholm, Sweden.
19. VERHAEGEN, F., VRAL, A., SEUNTJENS, J., SCHIPPER, N. W., DE RIDDER, L., and THIERENS, H., 1994, Scoring of radiation-induced micronuclei in cytokinesis-blocked human lymphocytes by automated image analysis. Cytometry, 17, 119-127.
20. VIAGGI, S., BRASELMANN, H., and NÜSSE, M., 1995, Flow cytometric analysis of micronuclei in the CD2± subpopulation of human lymphocytes enriched by magnetic separation. International Journal of Radiation Biology, 67, 193-202.