Alternative human liver transcripts of TCF7L2 bind to the gluconeogenesis regulator HNF4α at the protein level

Diabetologia

Volumn 57, Issue 4, 2014, Pages 785-796

  Neve, Bernadette ^a,b,c   Le Bacquer, Olivier ^a,b,d   Caron, Sandrine ^a,b,c,d   Huyvaert, Marlène ^a,b,c   Leloire, Audrey ^a,b,c   Poulain Godefroy, Odile ^a,b,c   Lecoeur, Cécile ^a,b,c   Pattou, François ^a,b,d   Staels, Bart ^a,b,c,d   Froguel, Philippe ^a,b,c,e  

^a EGID FR3508 European Genomics Institute of Diabetes   (France)

^b UNIV LILLE   (France)

^c INSTITUT PASTEUR DE LILLE   (France)

^d INSERM   (France)

^e HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

Gluconeogenesis; HNF4 ; Liver; TCF7L2 isoforms

Indexed keywords

GLUCOSE; INSULIN; SMALL INTERFERING RNA;

ADULT; ALLELE; ARTICLE; CELL CULTURE; CLINICAL ARTICLE; CONTROLLED STUDY; EXON; FEMALE; FOXO1 GENE; GENE; GENE CONTROL; GENE EXPRESSION; GENE REPRESSION; GENETIC TRANSCRIPTION; GENOTYPE; GLUCONEOGENESIS; GLUCOSE 6 PHOSPHATASE GENE; GLUCOSE METABOLISM; HEPATOCYTE NUCLEAR FACTOR 4A GENE; HEPATOCYTE NUCLEAR FACTOR 4ALPHA GENE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOPRECIPITATION; LUCIFERASE ASSAY; MALE; MIDDLE AGED; NON INSULIN DEPENDENT DIABETES MELLITUS; OBESITY; PATHOPHYSIOLOGY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; TRANSCRIPTION FACTOR 7 LIKE 2 GENE; TRANSCRIPTION REGULATION;

ADULT; BLOTTING, WESTERN; FEMALE; GLUCONEOGENESIS; HEP G2 CELLS; HEPATOCYTE NUCLEAR FACTOR 4; HUMANS; IMMUNOPRECIPITATION; LIVER; MALE; MIDDLE AGED; PROTEIN BINDING; PROTEIN ISOFORMS; TRANSCRIPTION FACTOR 7-LIKE 2 PROTEIN;

EID: 84896069312     PISSN: 0012186X     EISSN: 14320428     Source Type: Journal    
DOI: 10.1007/s00125-013-3154-z     Document Type: Article

References (34)

1. Cauchi S, Froguel P (2008) TCF7L2 genetic defect and type 2 diabetes. Curr Diab Rep 8:149-155
2. Rosengren AH, Braun M, Mahdi T et al (2012) Reduced insulin exocytosis in human pancreatic beta-cells with gene variants linked to type 2 diabetes. Diabetes 61:1726-1733
3. Schafer SA, Tschritter O, Machicao F et al (2007) Impaired glucagon-like peptide-1-induced insulin secretion in carriers of transcription factor 7-like 2 (TCF7L2) gene polymorphisms. Diabetologia 50:2443-2450 (Pubitemid 350060632)
4. Shu L, Matveyenko AV, Kerr-Conte J, Cho JH, McIntosh CH, Maedler K (2009) Decreased TCF7L2 protein levels in type 2 diabetes mellitus correlate with downregulation of GIP- and GLP-1 receptors and impaired beta-cell function. Hum Mol Genet 18:2388-2399
5. Villareal DT, Robertson H, Bell GI et al (2010) TCF7L2 variant rs7903146 affects the risk of type 2 diabetes by modulating incretin action. Diabetes 59:479-485
6. LeBacquer O, Kerr-Conte J, Gargani S et al (2012) TCF7L2 rs7903146 impairs islet function and morphology of non-diabetic individuals. Diabetologia 55:2677-2681
7. Savic D, Ye H, Aneas I, Park SY, Bell GI, Nobrega MA (2011) Alterations in TCF7L2 expression define its role as a key regulator of glucose metabolism. Genome Res 21:1417-1425
8. Osmark P, Hansson O, Jonsson A, Ronn T, Groop L, Renstrom E (2009) Unique splicing pattern of the TCF7L2 gene in human pancreatic islets. Diabetologia 52:850-854
9. Prokunina-Olsson L, Kaplan LM, Schadt EE, Collins FS (2009) Alternative splicing of TCF7L2 gene in omental and subcutaneous adipose tissue and risk of type 2 diabetes. PLoS One 4:e7231
10. Prokunina-Olsson L, Welch C, Hansson O et al (2009) Tissuespecific alternative splicing of TCF7L2. Hum Mol Genet 18:3795-3804
11. Mondal AK, Das SK, Baldini G et al (2010) Genotype and tissuespecific effects on alternative splicing of the transcription factor 7-like 2 gene in humans. J Clin Endocrinol Metab 95:1450-1457
12. LeBacquer O, Shu L, Marchand M et al (2011) TCF7L2 splice variants have distinct effects on β-cell turnover and function. Hum Mol Genet 20:1906-1915
13. Weise A, Bruser K, Elfert S et al (2010)Alternative splicing of Tcf7l2 transcripts generates protein variants with differential promoter-binding and transcriptional activation properties at Wnt/beta-catenin targets. Nucleic Acids Res 38:1964-1981
14. Locke JM, Da Silva Xavier G, Rutter GA, Harries LW (2012) An alternative polyadenylation signal in TCF7L2 generates isoforms that inhibit T cell factor/lymphoid-enhancer factor (TCF/LEF)-dependent target genes. Diabetologia 54:3078-3082
15. Lyssenko V, Lupi R, Marchetti P et al (2007) Mechanisms by which common variants in the TCF7L2 gene increase risk of type 2 diabetes. J Clin Invest 117:2155-2163 (Pubitemid 47219560)
16. Wegner L, Hussain MS, Pilgaard K et al (2008) Impact of TCF7L2 rs7903146 on insulin secretion and action in young and elderly Danish twins. J Clin Endocrinol Metab 93:4013-4019
17. Pilgaard K, Jensen CB, Schou JH et al (2009) The T allele of rs7903146 TCF7L2 is associated with impaired insulinotropic action of incretin hormones, reduced 24 h profiles of plasma insulin and glucagon, and increased hepatic glucose production in young healthy men. Diabetologia 52:1298-1307
18. Musso G, Gambino R, Pacini G, Pagano G, Durazzo M, Cassader M (2009) Transcription factor 7-like 2 polymorphism modulates glucose and lipid homeostasis, adipokine profile, and hepatocyte apoptosis in NASH. Hepatology 49:426-435
19. Torre C, Perret C, Colnot S (2011) Transcription dynamics in a physiological process: beta-catenin signaling directs liver metabolic zonation. Int J Biochem Cell Biol 43:271-278
20. Ip W, Shao W, Chiang YT, Jin T (2012) The Wnt signaling pathway effector TCF7L2 is up-regulated by insulin and represses hepatic gluconeogenesis. Am J Physiol Endocrinol Metab 303:E1166-E1176
21. Norton L, Fourcaudot M, Abdul-Ghani MA et al (2011) Chromatin occupancy of transcription factor 7-like 2 (TCF7L2) and its role in hepatic glucose metabolism. Diabetologia 54:3132-3142
22. Oh KJ, Park J, Kim SS, Oh H, Choi CS, Koo SH (2012) TCF7L2 modulates glucose homeostasis by regulating CREB- and FoxO1-dependent transcriptional pathway in the liver. PLoS Genet 8:e1002986
23. Lu M, Wan M, Leavens KF et al (2012) Insulin regulates liver metabolism in vivo in the absence of hepatic Akt and Foxo1. Nat Med 18:388-395
24. Liu H, Fergusson MM, Wu JJ et al (2011) Wnt signaling regulates hepatic metabolism. Sci Signal 4:ra6
25. Cauchi S, Meyre D, Dina C et al (2006) Transcription factor TCF7L2 genetic study in the French population: expression in human betacells and adipose tissue and strong association with type 2 diabetes. Diabetes 55:2903-2908 (Pubitemid 44923694)
26. Duval A, Rolland S, Tubacher E, Bui H, Thomas G, Hamelin R (2000) The human T cell transcription factor-4 gene: structure, extensive characterization of alternative splicings, and mutational analysis in colorectal cancer cell lines. Cancer Res 60:3872-3879 (Pubitemid 32204580)
27. Pukrop T, Gradl D, Henningfeld KA, Knochel W, Wedlich D, Kuhl M (2001) Identification of two regulatory elements within the high mobility group box transcription factor XTCF-4. J Biol Chem 276:8968-8978
28. Cattin AL, Le Beyec J, Barreau F et al (2009) Hepatocyte nuclear factor 4alpha, a key factor for homeostasis, cell architecture, and barrier function of the adult intestinal epithelium. Mol Cell Biol 29:6294-6308
29. Li Y, Mechin MC, van de Werve G (1999) Diabetes affects similarly the catalytic subunit and putative glucose-6-phosphate translocase of glucose-6-phosphatase. J Biol Chem 274:33866-33868 (Pubitemid 129511712)
30. Hirota K, Sakamaki J, Ishida J et al (2008) A combination of HNF-4 and Foxo1 is required for reciprocal transcriptional regulation of glucokinase and glucose-6-phosphatase genes in response to fasting and feeding. J Biol Chem 283:32432-32441
31. Hatzis P, van der Flier LG, van Driel MA et al (2008) Genome-wide pattern of TCF7L2/TCF4 chromatin occupancy in colorectal cancer cells.Mol Cell Biol 28:2732-2744 (Pubitemid 351542371)
32. Mokry M, Hatzis P, de Bruijn E et al (2010) Efficient double fragmentation ChIP-seq provides nucleotide resolution protein-DNA binding profiles. PLoS One 5:e15092
33. Frietze S, Wang R, Yao L et al (2012) Cell type-specific binding patterns reveal that TCF7L2 can be tethered to the genome by association with GATA3. Genome Biol 13:R52
34. Hecht A, Stemmler MP (2003) Identification of a promoterspecific transcriptional activation domain at the C terminus of the Wnt effector protein T cell factor 4. J Biol Chem 278:3776-3785 (Pubitemid 36801104)