Major groove binding track residues of the connection subdomain of human immunodeficiency virus type 1 reverse transcriptase enhance cDNA synthesis at high temperatures

Biochemistry

Volumn 52, Issue 51, 2013, Pages 9318-9328

  Matamoros, Tania ^a   Barrioluengo, Verónica ^a   Abia, David ^a   Menéndez Arias, Luis ^a  

^a CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; NUCLEIC ACIDS; TRANSCRIPTION; VIRUSES;

DNA BINDING AFFINITY; ELEVATED TEMPERATURE; HIGH TEMPERATURE; HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 REVERSE TRANSCRIPTASE; QUANTITATIVE REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; REFINED STRUCTURES; REVERSE TRANSCRIPTASES; REVERSE TRANSCRIPTION;

EFFICIENCY;

ALANINE; ARGININE; BACTERIOPHAGE DNA; COMPLEMENTARY DNA; DNA FRAGMENT; DNA POLYMERASE; DOUBLE STRANDED DNA; GLYCINE; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE; THYMIDINE TRIPHOSPHATE; DNA; MUTANT PROTEIN; PYRIMIDINE NUCLEOTIDE; RECOMBINANT PROTEIN; THYMIDINE 5'-TRIPHOSPHATE; VIRUS RNA;

ALPHA HELIX; ARTICLE; BASE MISPAIRING; BINDING AFFINITY; CRYSTAL STRUCTURE; DISSOCIATION; DNA BINDING; DNA SYNTHESIS; DNA TEMPLATE; ENZYME ACTIVITY; EQUILIBRIUM CONSTANT; GENE MUTATION; HIGH TEMPERATURE; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR DYNAMICS; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THERMOSTABILITY; WILD TYPE; AMINO ACID SUBSTITUTION; BINDING SITE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; CONFORMATION; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HEAT; KINETICS; METABOLISM; PROTEIN DATABASE; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; BINDING SITES; COMPUTER SIMULATION; DATABASES, PROTEIN; DNA; DNA, COMPLEMENTARY; ENZYME STABILITY; HIV REVERSE TRANSCRIPTASE; HIV-1; HOT TEMPERATURE; KINETICS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; RECOMBINANT PROTEINS; RIBONUCLEASE H, HUMAN IMMUNODEFICIENCY VIRUS; RNA, VIRAL; THYMINE NUCLEOTIDES;

EID: 84895778481     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi401390x     Document Type: Article

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