Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing

RNA

Volumn 19, Issue 7, 2013, Pages 958-970

  Mohr, Sabine ^a   Ghanem, Eman ^a   Smith, Whitney ^a   Sheeter, Dennis ^a   Qin, Yidan ^a   King, Olga ^a   Polioudakis, Damon ^a   Iyer, Vishwanath R ^a   Hunicke Smith, Scott ^a   Swamy, Sajani ^b   Kuersten, Scott ^b   Lambowitz, Alan M ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b ILLUMINA INC   (United States)

Author keywords

miRNA; Next generation sequencing; qRT PCR; Retrovirus; Transcriptome

Indexed keywords

COMPLEMENTARY DNA; HYBRID PROTEIN; MICRORNA; RNA DIRECTED DNA POLYMERASE; RNA LIGASE;

ARTICLE; BASE PAIRING; CAPILLARY ELECTROPHORESIS; DNA SYNTHESIS; INTRON; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; RNA STRUCTURE; THERMOPHILE; THERMOSTABILITY;

MIRNA; NEXT-GENERATION SEQUENCING; QRT-PCR; RETROVIRUS; TRANSCRIPTOME;

BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, COMPLEMENTARY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION PROFILING; GENE LIBRARY; GEOBACILLUS STEAROTHERMOPHILUS; HELA CELLS; HUMANS; INTRONS; MCF-7 CELLS; MICRORNAS; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; PERIPLASMIC BINDING PROTEINS; PLASMIDS; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA-DIRECTED DNA POLYMERASE; SEQUENCE ANALYSIS, RNA; TEMPERATURE;

BACTERIA (MICROORGANISMS);

EID: 84879286291     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.039743.113     Document Type: Article

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