Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis

Journal of Biotechnology

Volumn 150, Issue 3, 2010, Pages 299-306

  Yasukawa, Kiyoshi ^a   Mizuno, Masaki ^a   Konishi, Atsushi ^a   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

CDNA synthesis; Moloney murine leukaemia virus; Reverse transcriptase; Site directed mutagenesis; Stability

Indexed keywords

CDNA SYNTHESIS; HIGHEST TEMPERATURE; MOLONEY MURINE LEUKAEMIA VIRUS; MUTATION STRATEGY; POSITIVE CHARGES; REVERSE TRANSCRIPTASES; REVERSE TRANSCRIPTION; RNASE H; SITE DIRECTED MUTAGENESIS; SITE-DIRECTED MUTAGENSIS; THERMAL INACTIVATION; THERMAL STABILITY;

ESCHERICHIA COLI; MUTAGENESIS; THERMODYNAMIC STABILITY;

VIRUSES;

ASPARAGINE; COMPLEMENTARY DNA; GLUTAMINE; GLYCINE; LEUCINE; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE; TRYPTOPHAN;

ARTICLE; DNA SYNTHESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; MOLONEY LEUKEMIA ONCOVIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HOT TEMPERATURE; KINETICS; MODELS, MOLECULAR; MOLONEY MURINE LEUKEMIA VIRUS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RNA-DIRECTED DNA POLYMERASE;

ESCHERICHIA COLI; MURINAE;

EID: 78149465784     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.09.961     Document Type: Article

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