Tetrameric oligomerization of IκB kinase γ (IKKγ) is obligatory for IKK complex activity and NF-κB activation

Molecular and Cellular Biology

Volumn 23, Issue 6, 2003, Pages 2029-2041

  Tegethoff, Sebastian ^a   Behlke, Joachim ^a   Scheidereit, Claus ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LIPOPOLYSACCHARIDE; MONOMER; PHOSPHOTRANSFERASE; TETRAMER; BIOPOLYMER; CHUK PROTEIN, HUMAN; HYBRID PROTEIN; I KAPPA B; I KAPPA B BETA; I KAPPA B KINASE; IKBKB PROTEIN, HUMAN; IKBKE PROTEIN, HUMAN; NF KAPPAB INHIBITOR ALPHA; NF-KAPPAB INHIBITOR ALPHA; PROTEIN SERINE THREONINE KINASE; ZINC FINGER PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTORADIOGRAPHY; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CROSS LINKING; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; OLIGOMERIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; STOICHIOMETRY; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; GENE DELETION; HELA CELL; KIDNEY; MACROMOLECULE; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROCESSING; PROTEIN TERTIARY STRUCTURE; STRUCTURE ACTIVITY RELATION;

BIOPOLYMERS; CELL LINE; HELA CELLS; HUMANS; I-KAPPA B KINASE; I-KAPPA B PROTEINS; KIDNEY; MACROMOLECULAR SUBSTANCES; MODELS, MOLECULAR; NF-KAPPA B; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; STRUCTURE-ACTIVITY RELATIONSHIP; ZINC FINGERS;

EID: 0037332580     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.23.6.2029-2041.2003     Document Type: Article

References (46)

1. Agou, F., F. Ye, S. Goffinont, G. Courtois, S. Yamaoka, A. Israel, and M. Veron. 2002. NEMO trimerizes through its coiled-coil C-terminal domain. J. Biol. Chem. 277:17464-17475.
2. Behlke, J., and O. Ristau. 2002. A new approximate whole boundary solution of the Lamm differential equation for the analysis of sedimentation velocity experiments. Biophys. Chem. 95:59-68.
3. Behlke, J., O. Ristau, and H. J. Schonfeld. 1997. Nucleotide-dependent complex formation between the Escherichia coli chaperonins GroEL and GroES studied under equilibrium conditions. Biochemistry 36:5149-5156.
4. Cao, Y., G. Bonizzi, T. N. Seagroves, F. R. Greten, R. Johnson, E. V. Schmidt, and M. Karin. 2001. IKKα provides an essential link between RANK signaling and cyclin D1 expression during mammary gland development. Cell 107:763-775.
5. Chen, G., P. Cao, and D. V. Goeddel. 2002. TNF-induced recruitment and activation of the IKK complex require Cdc37 and Hsp90. Mol. Cell 9:401-410.
6. Courtois, G., A. Smahi, and A. Israel. 2001. NEMO/IKKγ: linking NF-κB to human disease. Trends Mol. Med. 7:427-430.
7. Delhase, M., M. Hayakawa, Y. Chen, and M. Karin. 1999. Positive and negative regulation of IκB kinase activity through IKKβ subunit phosphorylation. Science 284:309-313.
8. Devin, A., A. Cook, Y. Lin, Y. Rodriguez, M. Kelliher, and Z. Liu. 2000. The distinct roles of TRAF2 and RIP in IKK activation by TNF-R1: TRAF2 recruits IKK to TNF-R1 while RIP mediates IKK activation. Immunity 12:419-429.
9. Dumitru, C. D., J. D. Ceci, C. Tsatsanis, D. Kontoyiannis, K. Stamatakis, J. H. Lin, C. Patriotis, N. A. Jenkins, N. G. Copeland, G. Kollias, and P. N. Tsichlis. 2000. TNF-α induction by LPS is regulated posttranscriptionally via a Tp12/ERK-dependent pathway. Cell 103:1071-1083.
10. Ghosh, S., and M. Karin. 2002. Missing pieces in the NF-κB puzzle. Cell 109:S81-S96.
11. Hatada, E. N., D. Krappmann, and C. Scheidereit. 2000. NF-κB and the innate immune response. Curr. Opin. Immunol. 12:52-58.
12. βTrCP Mediated ubiquitination and degradation for the NF-κB precursor p105 and IκBα. Mol. Cell. Biol. 21:1024-1035.
13. Hu, Y., V. Baud, T. Oga, K. I. Kim, K. Yoshida, and M. Karin. 2001. IKKα controls formation of the epidermis independently of NF-κB. Nature 410:710-714.
14. Huang, T. T., S. L. Feinberg, S. Suryanarayanan, and S. Miyamoto. 2002. The zinc finger domain of NEMO is selectively required for NF-κB activation by UV radiation and topoisomerase inhibitors. Mol. Cell. Biol. 22:5813-5825.
15. Israel, A. 2000. The IKK complex: an integrator of all signals that activate NF-κB? Trends Cell Biol. 10:129-133.
16. Karin, M. 1999. How NF-κB is activated: the role of the IκB kinase (IKK) complex. Oncogene 18:6867-6874.
17. Kelliher, M. A., S. Grimm, Y. Ishida, F. Kuo, B. Z. Stanger, and P. Leder. 1998. The death domain kinase RIP mediates the TNF-induced NF-κB signal. Immunity 8:297-303.
18. Kim, D., Y. J. Lee, and P. M. Corry. 1992. Constitutive HSP70: oligomerization and its dependence on ATP binding. J. Cell. Physiol. 153:353-361.
19. Krappmann, D., E. N. Hatada, S. Tegethoff, J. Li, A. Klippel, K. Giese, P. A. Baeuerle, and C. Scheidereit. 2000. The IκB kinase (IKK) complex is tripartite and contains IKKγ but not IKAP as a regular component. J. Biol. Chem. 275:29779-29787.
20. Li, X., M. Commane, C. Burns, K. Vithalani, Z. Cao, and G. R. Stark. 1999. Mutant cells that do not respond to interleukin-1 (IL-1) reveal a novel role for IL-1 receptor-associated kinase. Mol. Cell. Biol. 19:4643-4652.
21. Li, Y., J. Kang, J. Friedman, L. Tarassishin, J. Ye, A. Kovalenko, D. Wallach, and M. S. Horwitz. 1999. Identification of a cell protein (FIP-3) as a modulator of NF-κB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis. Proc. Natl. Acad. Sci. USA 96:1042-1047.
22. Makris, C., J. L. Roberts, and M. Karin. 2002. The carboxyl-terminal region of IκB kinase γ (IKK-γ) is required for full IKK activation. Mol. Cell. Biol. 22:6573-6581.
23. May, M. J., F. D'Acquisto, L. A. Madge, J. Glockner, J. S. Pober, and S. Ghosh. 2000. Selective inhibition of NF-κB activation by a peptide that blocks the interaction of NEMO with the IκB kinase complex. Science 289:1550-1554.
24. May, M. J., and S. Ghosh. 1997. Rel/NF-κB and IκB proteins: an overview. Semin. Cancer Biol. 8:63-73.
25. May, M. J., R. B. Marienfeld, and S. Ghosh. 2002. Characterization of the IκB-kinase NEMO binding domain. J. Biol. Chem. 277:45992-46000.
26. Mercurio, F., B. W. Murray, A. Shevehenko, B. L. Bennett, D. B. Young, J. W. Li, G. Pascual, A. Motiwala, H. Zhu, M. Mann, and A. M. Manning. 1999. IκB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex. Mol. Cell. Biol. 19:1526-1538.
27. Mercurio, F., H. Zhu, B. W. Murray, A. Shevehenko, B. L. Bennett, J. Li, D. B. Young, M. Barbosa, M. Mann, A. Manning, and A. Rao. 1997. IKK-1 and IKK-2: cytokine-activated IκB kinases essential for NF-κB activation. Science 278:860-866.
28. Miller, B. S., and E. Zandi. 2001. Complete reconstitution of human IκB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKγ on the complex activity in the absence of stimulation. J. Biol. Chem. 276:36320-36326.
29. Poyet, J. L., S. M. Srinivasula, J. H. Lin, T. Fernandes-Alnemri, S. Yamaoka, P. N. Tsichlis, and E. S. Alnemri. 2000. Activation of the IκB kinases by RIP via IKKγ/NEMO-mediated oligomerization. J. Biol. Chem. 275:37966-37977.
30. Rothwarf, D. M., and M. Karin. 1999. The NF-κB activation pathway: a paradigm in information transfer from membrane to nucleus. Sci. Signal Transduction Knowledge Environ. 5:1.
31. Rothwarf, D. M., E. Zandi, G. Natoli, and M. Karin. 1998. IKK-γ is an essential regulatory subunit of the IκB kinase complex. Nature 395:297-300.
32. Silverman, N., and T. Maniatis. 2001. NF-κB signaling pathways in mammalian and insect innate immunity. Genes Dev. 15:2321-2342.
33. Silverman, N., R. Zhou, S. Stoven, N. Pandey, D. Hultmark, and T. Maniatis. 2000. A Drosophila IκB kinase complex required for Relish cleavage and antibacterial immunity. Genes Dev. 14:2461-2471.
34. Wolf, E., P, S. Kim, and B. Berger. 1997. MultiCoil: a program for predicting two- and three-stranded coiled coils. Protein Sci. 6:1179-1189.
35. Woronicz, J. D., X. Gao, Z. Cao, M. Rothe, and D. V. Goeddel. 1997. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 278:866-869.
36. Wulczyn, F. G., D. Krappmann, and C. Scheidereit. 1996. The NF-κB/Rel and IκB gene families: mediators of immune response and inflammation. J. Mol. Med. 74:749-769.
37. Xia, Y., C. Makris, B. Su, E. Li, J. Yang, G. R. Nemerow, and M. Karin. 2000. MEK kinase 1 is critically required for c-Jun N-terminal kinase activation by proinflammatory stimuli and growth factor-induced cell migration. Proc. Natl. Acad. Sci. USA 97:5243-5248.
38. Xiao, G., and S. C. Sun. 2000. Activation of IKKα and IKKβ through their fusion with HTLV-I tax protein. Oncogene 19:5198-5203.
39. Yamaoka, S., G. Courtois, C. Bessia, S. T. Whiteside, R. Weil, F. Agou, H. E. Kirk, R. J. Kay, and A. Israel. 1998. Complementation cloning of NEMO, a component of the IκB kinase complex essential for NF-κB activation. Cell 93:1231-1240.
40. Yang, J., Y. Lin, Z. Guo, J. Cheng, J. Huang, L. Deng, W. Liao, Z. Chen, Z. Liu, and B. Su. 2001. The essential role of MEKK3 in TNF-induced NF-κB activation. Nat. Immunol. 2:620-624.
41. Ye, J., X. Xie, L. Tarassishin, and M. S. Horwitz. 2000. Regulation of the NF-κB activation pathway by isolated domains of FIP3/IKK-γ, a component of the IκB-α kinase complex. J. Biol. Chem. 275:9882-9889.
42. Yin, L., L. Wu, H. Wesche, C. D. Arthur, J. M. White, D. V. Goeddel, and R. D. Schreiber. 2001. Defective lymphotoxin-beta receptor-induced NF-κB transcriptional activity in NIK-deficient mice. Science 291:2162-2165.
43. 2-terminal kinase regulation but does not cause a measurable defect in NF-κB activation. Proc. Natl. Acad. Sci. USA 97:7272-7277.
44. Zandi, E., and M. Karin. 1999. Bridging the gap: composition, regulation, and physiological function of the IκB kinase complex. Mol. Cell. Biol. 19:4547-4551.
45. Zandi, E., D. M. Rothwarf, M. Delhase, M. Hayakawa, and M. Karin. 1997. The IκB kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ, necessary for IκB phosphorylation and NF-κB activation. Cell 91:243-252.
46. Zhang, S. Q., A. Kovalenko, G. Cantarella, and D. Wallach. 2000. Recruitment of the IKK signalosome to the p55 TNF receptor: RIP and A20 bind to NEMO (IKKγ) upon receptor stimulation. Immunity 12:301-311.