Intermolecular disulfide bond formation in the NEMO dimer requires Cys54 and Cys347

Biochemical and Biophysical Research Communications

Volumn 367, Issue 1, 2008, Pages 103-108

  Herscovitch, Melanie ^a   Comb, William ^a   Ennis, Thomas ^a   Coleman, Kate ^a   Yong, Sheila ^a   Armstead, Brinda ^a   Kalaitzidis, Demetrios ^a   Chandani, Sushil ^a   Gilmore, Thomas D ^a  

^a Boston University   (United States)

Author keywords

Cysteine; Disulfide bond; Hydrogen peroxide; IKK; Molecular modeling; NEMO; NF B

Indexed keywords

CYSTEINE; DISULFIDE; DNA; HYDROGEN PEROXIDE; I KAPPA B KINASE GAMMA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; CELL TYPE; DISULFIDE BOND; DNA BINDING; ENZYME ACTIVITY; MOLECULAR DYNAMICS; MOLECULAR MODEL; MOUSE; MUTANT; NONHUMAN; OXIDATION REDUCTION REACTION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE;

ANIMALS; BASE SEQUENCE; CELL NUCLEUS; CELLS, CULTURED; CYSTEINE; DIMERIZATION; DISULFIDES; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN PEROXIDE; I-KAPPA B KINASE; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MODELS, MOLECULAR; MUTATION; OXIDATION-REDUCTION; PROTEIN BINDING; TUMOR NECROSIS FACTOR-ALPHA;

EID: 38049070287     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.12.123     Document Type: Article

References (21)

1. Perkins N.D. Post-translational modifications regulating the activity and function of the NF-κB pathway. Oncogene 25 (2006) 6717-6730
2. Yamamoto Y., Kim D.W., Kwak Y.T., Prajapti S., Verma U., and Gaynor R.B. IKKγ/NEMO facilitates the recruitment of the IκBα proteins into the IκB kinase complex. J. Biol. Chem. 276 (2001) 36327-36336
3. Sebban H., Yamaoka S., and Courtois G. Posttranslational modifications of NEMO and its partners in NF-κB signaling. Trends Cell Biol. 16 (2006) 569-577
4. Agou F., Ye F., Goffinont S., Courtois G., Yamaoka S., Israël A., and Veron M. NEMO trimerizes through its coiled-coil C-terminal domain. J. Biol. Chem. 277 (2002) 17464-17475
5. Agou F., Traincard F., Vinolo E., Courtois G., Yamaoka S., Israël A., and Veron M. The trimerization domain of NEMO is composed of the interacting C-terminal CC2 and LZ coiled-coil subdomains. J. Biol. Chem. 279 (2004) 27861-27869
6. Fontan E., Traincard F., Levy S.G., Yamaoka S., Veron M., and Agou F. NEMO oligomerization in the dynamic assembly of the IκB kinase core complex. FEBS J. 274 (2007) 2540-2541
7. Tegethoff S., Behlke J., and Scheidereit C. Tetrameric oligomerization of IκB kinase γ (IKKγ) is obligatory for IKK complex activity and NF-κB activation. Mol. Cell. Biol. 23 (2003) 2029-2041
8. Marienfeld R.B., Palkowitsch L., and Ghosh S. Dimerization of the IκB kinase-binding domain of NEMO is required for tumor necrosis factor α-induced NF-κB activity. Mol. Cell. Biol. 26 (2006) 9209-9219
9. Vinolo E., Sebban H., Chaffotte A., Israel A., Courtois G., Veron M., and Agou F. A point mutation in NEMO associated with anhidrotic ectodermal dysplasia with immunodeficiency pathology results in destabilization of the oligomer and reduces lipopolysaccharide- and tumor necrosis factor-mediated NF-κB activation. J. Biol. Chem. 281 (2006) 6334-6348
10. Bubici C., Papa S., Dean K., and Franzoso G. Mutual cross-talk between reactive oxygen species and NF-κB: molecular basis and biological significance. Oncogene 25 (2006) 6731-6748
11. Liang M.-C., Bardhan S., Pace E.A., Rosman D., Beutler J.A., Porco Jr. J.A., and Gilmore T.D. Inhibition of transcription factor NF-κB signaling proteins IKKβ and p65 through specific cysteine residues by epoxyquinone A monomer: correlation with its anti-cancer cell growth activity. Biochem. Pharmacol. 71 (2006) 634-645
12. Gapuzan M.-E., Yufit P.V., and Gilmore T.D. Immortalized embryonic mouse fibroblasts lacking the RelA subunit of transcription factor NF-κB have a malignantly transformed phenotype. Oncogene 21 (2002) 2484-2492
13. Li C., Bardhan S., Pace E.A., Liang M.-C., Gilmore T.D., and Porco Jr. J.A. Angiogenesis inhibitor epoxyquinol A: total synthesis and inhibition of transcription factor NF-κB. Org. Lett. 4 (2002) 3267-3270
14. Kalaitzidis D., Davis R.E., Rosenwald A., Staudt L.M., and Gilmore T.D. The human B-cell lymphoma cell line RC-K8 has multiple genetic alterations that dysregulate the Rel/NF-κB signal transduction pathway. Oncogene 21 (2002) 8759-8768
15. Liang M.-C., Bardhan S., Li C., Pace E.A., Porco Jr. J.A., and Gilmore T.D. Jesterone dimer, a synthetic derivative of the fungal metabolite jesterone, blocks activation of transcription factor nuclear factor κB by inhibiting the inhibitor of κB kinase. Mol. Pharmacol. 64 (2003) 123-131
16. Senkevich T.G., White C.L., Koonin E.V., and Moss B. Complete pathway for protein disulfide bond formation encoded by poxviruses. Proc. Natl. Acad. Sci. USA 99 (2002) 6667-6672
17. Makris C., Roberts J.L., and Karin M. The carboxyl-terminal region of IKKγ is required for full IKK activation. Mol. Cell. Biol. 22 (2002) 6573-6581
18. Ghezzi P. Oxidoreduction of protein thiols in redox regulation. Biochem. Soc. Trans. 330 (2005) 1378-1381
19. Drew D., Shimada E., Huynh K., Bergqvist S., Talwar R., Karin M., and Ghosh G. Inhibitor κB kinase β binding by inhibitor κB kinase γ. Biochemistry 46 (2007) 12482-12490
20. Palkowitsch L., Leidner J., Ghosh S., and Marienfeld R.B. Phosphorylation of serine 68 in the IκB kinase (IKK)-binding domain of NEMO interferes with the structure of the IKK complex and tumor necrosis factor-α-induced NF-κB activity. J. Biol. Chem. 283 (2008) 76-86
21. Korn S.H., Wouters E.F.M., Vos N., and Janssen-Heininger Y.M.W. Cytokine-induced activation of NF-κB is inhibited by hydrogen peroxide through oxidative inactivation of IκB kinase. J. Biol. Chem. 276 (2001) 35693-35700