Identification of the rate-limiting step of the peroxygenase reactions catalyzed by the thermophilic cytochrome P450 from Sulfolobus tokodaii strain 7

FEBS Journal

Volumn 281, Issue 5, 2014, Pages 1409-1416

  Hayakawa, Shohei ^a   Matsumura, Hirotoshi ^a,b   Nakamura, Nobuhumi ^a   Yohda, Masafumi ^a   Ohno, Hiroyuki ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

cytochrome P450; peroxygenase reaction; pH dependence; rate limiting step; site directed mutagenesis

Indexed keywords

CYTOCHROME P450; ETHYLBENZENE; HYDROGEN PEROXIDE; IRON COMPLEX; OXYGENASE; PEROXYGENASE; STYRENE; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; DEPROTONATION; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME KINETICS; EPOXIDATION; HYDROXYLATION; LEWIS ACIDITY; MICHAELIS CONSTANT; NONHUMAN; PH; PRIORITY JOURNAL; PROTON TRANSPORT; STEADY STATE; SULFOLOBUS TOKODAII; TURNOVER TIME;

CRENARCHAEOTA; SULFOLOBUS TOKODAII STR. 7;

CYTOCHROME P450; PEROXYGENASE REACTION; PH DEPENDENCE; RATE-LIMITING STEP; SITE-DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ARCHAEAL PROTEINS; BENZENE DERIVATIVES; CYTOCHROME P-450 ENZYME SYSTEM; HEME; HYDROGEN-ION CONCENTRATION; HYDROXYLATION; KINETICS; LEWIS ACIDS; MIXED FUNCTION OXYGENASES; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SULFOLOBUS;

EID: 84895468123     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12712     Document Type: Article

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