Stabilizing plasmid copy number to improve recombinant protein production

Biotechnology and Bioengineering

Volumn 77, Issue 2, 2002, Pages 142-147

  Grabherr, Reingard ^a   Nilsson, Erik ^b,c   Striedner, Gerald ^a   Bayer, Karl ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^b LINKÖPING UNIVERSITY   (Sweden)

^c LINKÖPING INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Escherichia coli; Plasmid Co E1; Recombinant; Replication; RNA I RNA II

Indexed keywords

BACTERIA; CELL CULTURE; METABOLISM; OPTIMIZATION; RNA; VECTORS;

PROTEIN PRODUCTION;

PROTEINS;

RECOMBINANT PROTEIN; RNA; TRANSFER RNA;

ARTICLE; BACTERIUM CULTURE; BIOREACTOR; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROTEIN PURIFICATION; RNA REPLICATION; SEQUENCE HOMOLOGY;

BACTERIAL PROTEINS; BASE SEQUENCE; BIOREACTORS; CULTURE MEDIA; DNA PRIMERS; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PLASMIDS; RECOMBINANT PROTEINS; REPLICATION ORIGIN; RNA, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VECTORS;

EID: 0037137907     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10104     Document Type: Article

References (19)

1. Plasmid encoded protein: The principal factor in the "metabolic burden" associated with recombinant bacteria
2. Off-line quantitative monitoring of plasmid copy number in bacterial fermentation by capillary electrophoresis
3. Control of ColE1 plasmid replication by antisense RNA
4. Metabolic approaches for the optimisation of recombinant fermentation processes
5. Kinetics studies for the optimization of recombinant
6. Antisense RNA regulation in prokaryotes: Rapid RNA/RNA interaction facilitated by a general U-turn loop structure
7. U-turns and regulatory RNAs
8. Metabolic load and heterologous gene expression
9. The effect of loop size in antisense and target RNAs on the efficiency of antisense RNA control
10. Rapid and sensitive enzyme immuno assay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies
11. Use of the bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
12. Metabolic approach to the optimisation of recombinant fermentation processes
13. Amplification of ColE1 related plasmids in recombinant cultures of Escherichia coli after IPTG induction
14. Plasmid DNA-replication
15. Control of ColE1 plasmid replication: Initial interaction of RNA I and the primer transcript is reversible
16. Control of ColE1 plasmid replication, intermediates in the binding of RNA I and RNA II
17. Control of ColE1 plasmid replication, interaction of Rom protein with an unstable complex formed by RNA I and RNA II
18. Replication regulation of ColE1 like plasmids in amino acid-starved Escherichia coli
19. What does the homology between E. coli tRNAs and RNAs controlling ColE1 plasmid replication mean?