Opportunities and challenges for gut microbiome studies in the Indian population

Microbiome

Volumn 1, Issue 1, 2013, Pages

  Shetty, Sudarshan A ^a   Marathe, Nachiket P ^a   Shouche, Yogesh S ^a  

^a NATIONAL CENTRE FOR CELL SCIENCE   (India)

Author keywords

Diet; Diversity; Genetics; Gut microbiome; Indian population

Indexed keywords

CIPROFLOXACIN; HLA DQ2 ANTIGEN; HLA DQ8 ANTIGEN; RNA 16S;

CELIAC DISEASE; DIABETES MELLITUS; DIETARY INTAKE; ETHNICITY; GENETIC VARIABILITY; GEOGRAPHIC DISTRIBUTION; HEALTH STATUS; HUMAN; INDIAN; INFLAMMATORY BOWEL DISEASE; INTESTINE FLORA; METAGENOMICS; MICROBIAL COLONIZATION; MICROBIAL COMMUNITY; MICROBIAL GENE; MICROBIOME; OBESITY; PRIORITY JOURNAL; REVIEW; RISK ASSESSMENT; RISK FACTOR;

EID: 84894182162     PISSN: None     EISSN: 20492618     Source Type: Journal    
DOI: 10.1186/2049-2618-1-24     Document Type: Review

References (104)

1. Mitsou EK, Kirtzalidou E, Oikonomou I, Liosis G, Kyriacou A. Fecal microflora of Greek healthy neonates. Anaerobe 2008, 14:94-101. 10.1016/j.anaerobe.2007.11.002, 18207437.
2. De Filippo C, Cavalieri D, Di Paola M, Ramazzotti M, Poullet JB, Massart S, Collini S, Pieraccini G, Lionetti P. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. PNAS 2010, 107:14691-14696. 10.1073/pnas.1005963107, 2930426, 20679230.
3. Hopkins MJ, Sharp R, Macfarlane GT. Age and disease related changes in intestinal bacterial populations assessed by cell culture, 16S rRNA abundance, and community cellular fatty acid profiles. Gut 2001, 48:198-205. 10.1136/gut.48.2.198, 1728209, 11156640.
4. Khachatryan ZA, Ktsoyan ZA, Manukyan GP, Kelly D, Ghazaryan KA, Aminov RI. Predominant role of host genetics in controlling the composition of gut microbiota. PLoS ONE 2008, 3:e3064. 10.1371/journal.pone.0003064, 2516932, 18725973.
5. Turnbaugh PJ, Ridaura VK, Faith JJ, Rey FE, Knight R, Gordon JI. The effect of diet on the human gut microbiome: a metagenomic analysis in humanized gnotobiotic mice. Sci Transl Med 2009, 1:6ra-14ra.
6. Turnbaugh PJ, Quince C, Faith JJ, McHardy AC, Yatsunenko T, Niazi F, Affourtit J, Egholm M, Henrissat B, Knight R, Gordon JI. Organismal, genetic, and transcriptional variation in the deeply sequenced gut microbiomes of identical twins. PNAS 2010, 107:7503-7508. 10.1073/pnas.1002355107, 2867707, 20363958.
7. Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, Magris M, Hidalgo G, Baldassano RN, Anokhin AP, Heath AC, Warner B, Reeder J, Kuczynski J, Caporaso JG, Lozupone CA, Lauber C, Clemente JC, Knights D, Knight R, Gordon JI. Human gut microbiome viewed across age and geography. Nature 2012, 486:222-227. 3376388, 22699611.
8. Karlsson FH, Tremaroli V, Nookaew I, Bergström G, Behre CJ, Fagerberg B, Nielsen J, Bäckhed F. Gut metagenome in European women with normal, impaired and diabetic glucose control. Nature 2013, 498:99-103. 10.1038/nature12198, 23719380.
9. Mathur P, Shah B. Research priorities for prevention and control of on communicable diseases in India. Indian J Community Med 2011, 36:S72-S77. 10.4103/0970-0218.94713, 3354900, 22628917.
10. Majumder PP. Genomic inferences on peopling of south Asia. Curr Opin Genet Dev 2008, 18:280-284. 10.1016/j.gde.2008.07.003, 18662778.
11. Bamshad M, Kivisild T, Watkins WS, Dixon ME, Ricker CE, Rao BB, Naidu JM, Prasad BV, Reddy PG, Rasanayagam A, Papiha SS, Villems R, Redd AJ, Hammer MF, Nguyen SV, Carroll ML, Batzer MA, Jorde LB. Genetic Evidence on the origins of Indian caste populations. Genome Res 2001, 11:994-1004. 10.1101/gr.GR-1733RR, 311057, 11381027.
12. Reich D, Thangaraj K, Patterson N, Price AL, Singh L. Reconstructing Indian population history. Nature 2009, 461:489-494. 10.1038/nature08365, 2842210, 19779445.
13. Watkins WS, Thara R, Mowry BJ, Zhang Y, Witherspoon DJ, Tolpinrud W, Bamshad MJ, Tiripati S, Padmavati R, Smith H, Nancarrow D, Filippich C, Jorde LB. Genetic variation in South Indian castes: evidence from Y-chromosome, mitochondrial, and autosomal polymorphisms. BMC Genet 2008, 12:9-86.
14. Yajnik CS, Yudkin JS. The Y-Y paradox. Lancet 2004, 363(9403):163. 10.1016/S0140-6736(03)15269-5, 14726172.
15. Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA. Diversity of the human intestinal microbial flora. Science 2005, 308:1635. 10.1126/science.1110591, 1395357, 15831718.
16. Bettelheim KA, Breardon A, Faiers MC, O'Farrell SM. The origin of O serotypes of Escherichia coli in babies after normal delivery. J Hyg (Lond) 1974, 72:67-70.
17. Brook I, Barett C, Brinkman C, Martin W, Finegold S. Aerobic and anaerobic bacterial flora of the maternal cervix and newborn gastric fluid and conjunctiva: a prospective study. Pediatrics 1979, 63:451-455.
18. Collado MC, Isolauri E, Laitinen K, Salminen S. Effect of mother's weight on infant's microbiota acquisition, composition, and activity during early infancy: a prospective follow-up study initiated in early pregnancy. Am J Clin Nutr 2010, 92:1023-1030. 10.3945/ajcn.2010.29877, 20844065.
19. Palmer C, Bik EM, DiGiulio DB, Relman DA, Brown PO. Development of the human infant intestinal microbiota. PLoS Biol 2007, 5:e177. 10.1371/journal.pbio.0050177, 1896187, 17594176.
20. Ley RE, Peterson DA, Gordon JI. Ecological and evolutionary forces shaping microbial diversity in the human intestine. Cell 2006, 124:837-848. 10.1016/j.cell.2006.02.017, 16497592.
21. Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, Nielsen T, Pons N, Levenez F, Yamada T, Mende DR, Li J, Xu J, Li S, Li D, Cao J, Wang B, Liang H, Zheng H, Xie Y, Tap J, Lepage P, Bertalan M, Batto JM, Hansen T, Le Paslier D, Linneberg A, Nielsen HB, Pelletier E, Renault P, et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 2010, 464:59-65. 10.1038/nature08821, 20203603.
22. Bocci V. The neglected organ: bacterial flora has a crucial immunostimulatory role. Perspect Biol Med 1992, 35:251-260.
23. Vrieze A, Holleman F, Zoetendal EG, de Vos WM, Hoekstra JB, Nieuwdorp M. The environment within: how gut microbiota may influence metabolism and body composition. Diabetologia 2010, 53:606-613. 10.1007/s00125-010-1662-7, 2830587, 20101384.
24. Cantarel BL, Lombard V, Henrissat B. Complex carbohydrate utilization by the healthy human microbiome. PLoS ONE 2012, 7:e28742. 10.1371/journal.pone.0028742, 3374616, 22719820.
25. Weinstein PD, Cebra JJ. The preference for switching to IgA expression by Peyer's patch germinal center B cells is likely due to the intrinsic influence of their microenvironment. J Immunol 1991, 147:4126-4135.
26. Cebra JJ. Influences of microbiota on intestinal immune system development. Am J Clin Nutr 1999, 69:1046S-1051S.
27. Ley RE, Bäckhed F, Turnbaugh P, Lozupone CA, Knight RD, Gordon JI. Obesity alters gut microbial ecology. PNAS 2005, 102:11070-11075. 10.1073/pnas.0504978102, 1176910, 16033867.
28. Bäckhed F, Ding H, Wang T, Hooper LV, Koh GY, Nagy A, Semenkovich CF, Gordon JI. The gut microbiota as an environmental factor that regulates fat storage. PNAS 2004, 101:15718-15723. 10.1073/pnas.0407076101, 524219, 15505215.
29. Stappenbeck TS, Hooper LV, Gordon JI. Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells. PNAS 2002, 99:15451-15455. 10.1073/pnas.202604299, 137737, 12432102.
30. Xu J, Mahowald MA, Ley RE, Lozupone CA, Hamady M, Martens EC, Henrissat B, Coutinho PM, Minx P, Latreille P, Cordum H, Van Brunt A, Kim K, Fulton RS, Fulton LA, Clifton SW, Wilson RK, Knight RD, Gordon JI. Evolution of symbiotic bacteria in the distal human intestine. PLoS Biol 2007, 5:e156. 10.1371/journal.pbio.0050156, 1892571, 17579514.
31. Hooper LV, Midtvedt T, Gordon JI. How host-microbial interactions shape the nutrient environment of the mammalian intestine. Annu Rev Nutr 2002, 22:283-307. 10.1146/annurev.nutr.22.011602.092259, 12055347.
32. MacDonald TT, Gordon JN. Bacterial regulation of intestinal immune responses. Gastroenterol Clin North Am 2005, 34:401-412. 10.1016/j.gtc.2005.05.012, 16084304.
33. Nicholson JK, Holmes E, Kinross J, Burcelin R, Gibson G, Jia W, Pettersson S. Host-gut microbiota metabolic interactions. Science 2012, 336:1262-1267. 10.1126/science.1223813, 22674330.
34. Larsen N, Vogensen FK, van den Berg FW, Nielsen DS, Andreasen AS, Pedersen BK, Al-Soud WA, Sørensen SJ, Hansen LH, Jakobsen M. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PLoS ONE 2010, 5:e9085. 10.1371/journal.pone.0009085, 2816710, 20140211.
35. Qin J, Li Y, Cai Z, Li S, Zhu J, Zhang F, Liang S, Zhang W, Guan Y, Shen D, Peng Y, Zhang D, Jie Z, Wu W, Qin Y, Xue W, Li J, Han L, Lu D, Wu P, Dai Y, Sun X, Li Z, Tang A, Zhong S, Li X, Chen W, Xu R, Wang M, Feng Q, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes. Nature 2012, 490:55-60. 10.1038/nature11450, 23023125.
36. Kashyap P, Farrugia G. Oxidative stress: key player in gastrointestinal complications of diabetes. Neurogastroenterol Motil 2011, 123:111-114. 201.
37. Armougom F, Henry M, Vialettes B, Raccah D, Raoult D. Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and methanogens in anorexic patients. PLoS ONE 2009, 4(9):e7125. 10.1371/journal.pone.0007125, 2742902, 19774074.
38. Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, Gordon JI. An Obesity-associated gut microbiome with increased capacity for energy harvest. Nature 2006, 444:1027-1031. 10.1038/nature05414, 17183312.
39. Friswell M, Campbell B, Rhodes J. The role of bacteria in the pathogenesis of inflammatory bowel disease. Gut and Liver 2010, 4:295-306. 10.5009/gnl.2010.4.3.295, 2956340, 20981205.
40. Greenblum S, Turnbaugh PJ, Borenstein E. Metagenomic systems biology of the human gut microbiome reveals topological shifts associated with obesity and inflammatory bowel disease. Proc Natl Acad Sci USA 2012, 109:594-599. 10.1073/pnas.1116053109, 3258644, 22184244.
41. Mearin ML, Ivarsson A, Dickey W. Coeliac disease: is it time for mass screening?. Best Pract Res Clin Gastroenterol 2005, 19:441-452. 10.1016/j.bpg.2005.02.004, 15925848.
42. Collado MC, Donat E, Ribes-Koninckx C, Calabuig M, Sanz Y. Specific duodenal and faecal bacterial groups associated with paediatric coeliac disease. J Clin Pathol 2009, 62:264-269. 10.1136/jcp.2008.061366, 18996905.
43. Schippa S, Iebba V, Barbato M, Di Nardo G, Totino V, Checchi MP, Longhi C, Maiella G, Cucchiara S, Conte MP. A distinctive 'microbial signature' in celiac pediatric patients. BMC Microbiol 2010, 10:175. 10.1186/1471-2180-10-175, 2906462, 20565734.
44. Pandey PK, Verma P, Kumar H, Bavdekar A, Patole MS, Shouche YS. Comparative analysis of fecal microflora of healthy full-term Indian infants born with different methods of delivery (vaginal vs cesarean): Acinetobacter sp. prevalence in vaginally born infants. J Biosci 2012, 37:989-998. 10.1007/s12038-012-9268-5, 23151789.
45. Floros JD, Newsome R, Fisher W, Barbosa-Canovas GV, Chen H, Dunne CP, German JB, Hall RL, Heldman DR, Karwe MV, Knabel SJ, Labuza TP, Lund DB, Newell-McGloughlin M, Robinson JL, Sebranek JG, Shewfelt RL, Tracy WF, Weaver CM, Ziegler GR. Feeding the world today and tomorrow: the importance of food science and technology. Compr. Rev. Food Sci. Food Safety 2010, 9:572-599.
46. Balamurugan R, Magne F, Balakrishnan D, Suau A, Ramani S, Kang G, Ramakrishna BS. Faecal bifidobacteria in Indian neonates & the effect of asymptomatic rotavirus infection during the first month of life. Indian J Med Res 2010, 132:721-727. 3102461, 21245621.
47. Balamurugan R, Janardhan HP, George S, Chittaranjan SP, Ramakrishna BS. Bacterial succession in the colon during childhood and adolescence: molecular studies in a southern Indian village. Am J Clin Nutr 2008, 88:1643-1647. 10.3945/ajcn.2008.26511, 19064526.
48. Marathe NM, Shetty SA, Lanjekar V, Ranade D, Shouche YS. Changes in human gut flora with age: an Indian familial study. BMC Microbiol 2012, 12:222. 10.1186/1471-2180-12-222, 3511239, 23013146.
49. Kabeerdoss J, Devi RS, Mary RR, Ramakrishna BS. Faecal microbiota composition in vegetarians: comparison with omnivores in a cohort of young women in southern India. Br J Nutr 2011, 20:1-5.
50. Singh KS. India's Communities National Series: People of India 1998, New Delhi: Oxford University Press.
51. Rajkumar R, Kashyap VK. Genetic structure of four socio-culturally diversified caste populations of southwest India and their affinity with related Indian and global groups. BMC Genet 2004, 5:23. 515297, 15317657.
52. Balamurugan R, George G, Kabeerdoss J, Hepsiba J, Chandragunasekaran AM, Ramakrishna BS. Quantitative differences in intestinal Faecalibacterium prausnitzii in obese Indian children. Br J Nutr 2010, 103:335-338. 10.1017/S0007114509992182, 19849869.
53. Patil DP, Dhotre DP, Chavan SG, Sultan A, Jain DS, Lanjekar VB, Gangawani J, Shah PS, Todkar JS, Shah S, Ranade DR, Patole MS, Shouche YS. Molecular analysis of gut microbiota in obesity among Indian individuals. J Biosci 2012, 37:647-657. 10.1007/s12038-012-9244-0, 22922190.
54. Frank DN, St Amand AL, Feldman RA, Boedeker EC, Harpaz N, Pace NR. Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. PNAS 2007, 104:13780-13785. 10.1073/pnas.0706625104, 1959459, 17699621.
55. Willing BP, Dicksved J, Halfvarson J, Andersson AF, Lucio M, Zheng Z, Järnerot G, Tysk C, Jansson JK, Engstrand L. A pyrosequencing study in twins shows that gastrointestinal microbial profiles vary with inflammatory bowel disease phenotypes. Gastroenterology 2010, 139:1844-1854. 10.1053/j.gastro.2010.08.049, 20816835.
56. Verma R, Verma AK, Ahuja V, Paul J. Real-time analysis of mucosal flora in patients with inflammatory bowel disease in India. J Clin Microbiol 2010, 48:4279-4282. 10.1128/JCM.01360-10, 3020854, 20861337.
57. Di Cagno R, De Angelis M, De Pasquale I, Ndagijimana M, Vernocchi P, Ricciuti P, Gagliardi F, Laghi L, Crecchio C, Guerzoni ME, Gobbetti M, Francavilla R. Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization. BMC Microbiol 2011, 11:219. 10.1186/1471-2180-11-219, 3206437, 21970810.
58. Collado MC, Donat E, Ribes-Koninckx C, Calabuig M, Sanz Y. Imbalances in faecal and duodenal Bifidobacterium species composition in active and non-active coeliac disease. BMC Microbiol 2008, 8:232. 10.1186/1471-2180-8-232, 2635381, 19102766.
59. Sellitto M, Bai G, Serena G, Fricke WF, Sturgeon C, Gajer P, White JR, Koenig SS, Sakamoto J, Boothe D, Gicquelais R, Kryszak D, Puppa E, Catassi C, Ravel J, Fasano A. Proof of concept of microbiome-metabolome analysis and delayed gluten exposure on celiac disease autoimmunity in genetically at-risk infants. PLoS ONE 2012, 7:e33387. 10.1371/journal.pone.0033387, 3303818, 22432018.
60. Song SJ, Lauber C, Costello EK, Lozupone CA, Humphrey G, Berg-Lyons D, Caporaso JG, Knights D, Clemente JC, Nakielny S, Gordon JI, Fierer N, Knight R. Cohabiting family members share microbiota with one another and with their dogs. Elife 2013, 2:e00458. 10.7554/eLife.00458, 3628085, 23599893.
61. Muegge BD, Kuczynski J, Knights D, Clemente JC, González A, Fontana L, Henrissat B, Knight R, Gordon JI. Diet drives convergence in gut microbiome functions across mammalian phylogeny and within humans. Science 2011, 332:970-974. 10.1126/science.1198719, 3303602, 21596990.
62. Balamurugan R, Janardhan HP, George S, Raghava MV, Muliyil J, Ramakrishna BS. Molecular studies of fecal anaerobic commensal bacteria in acute diarrhea in children. J Pediatr Gastroenterol Nutr 2008, 46:514-519. 10.1097/MPG.0b013e31815ce599, 18493205.
63. Balamurugan R, Mary RR, Chittaranjan S, Jancy H, Shobana Devi R, Ramakrishna BS. Low levels of faecal lactobacilli in women with iron-deficiency anaemia in south India. Br J Nutr 2010, 104:931-934. 10.1017/S0007114510001637, 20447323.
64. Gupta SS, Mohammed MH, Ghosh TS, Kanungo S, Nair GB, Mande SS. Metagenome of the gut of a malnourished child. Gut Pathogens 2011, 3:7. 10.1186/1757-4749-3-7, 3115890, 21599906.
65. Pandey PK, Siddharth J, Verma P, Bavdekar A, Patole MS, Shouche YS. Molecular typing of fecal eukaryotic microbiota of human infants and their respective mothers. J Biosci 2012, 37:221-226. 10.1007/s12038-012-9197-3, 22581327.
66. Zoetandal EG, Akkermans ADL, Akkermans-van Vliet WM, de Visser JA, de Vos WM. The host genotype affects the bacterial community in the human gastrointestinal tract. Microb. Ecol. Health Disease 2001, 13:129-134.
67. Gadgil M, Joshi NV, Shambu Prasad UV, Manoharan S. Suresh Patil: peopling of India. The Indian Human Heritage 1997, Hyderabad, India: Universities Press, Balasubramanian D, Appaji Rao N.
68. Guzder SN, Jain R, Sharma N, Gopalkrishnan C, Shah Y, Morawala-Patell V. The A V E S T A G E N O M E project™ - a discovery model for disease genomics and beyond. Genome Biol 2010, 11(Suppl 1):P16.
69. Demographic and health aspects of the Parsi community [http://unescoparzor.com/index.php?option=com_content&view=article&id=70&phpMyAdmin=sajdmQ8uXCI6iyWREItGtdZWr-2&Itemid=61].
70. Tito RY, Macmil S, Wiley G, Najar F, Cleeland L, Qu C, Wang P, Romagne F, Leonard S, Ruiz AJ, Reinhard K, Roe BA, Lewis CM. Phylotyping and functional analysis of two ancient human microbiomes. PLoS ONE 2008, 3:e3703. 10.1371/journal.pone.0003703, 2577302, 19002248.
71. Bhasin MK, Walter H. Genetics of Castes and Tribes of India 2001, Delhi; India: Kamla-Raj Enterprises.
72. Maji S, Krithika S, Vasulu TS. Phylogeographic distribution of mitochondiral DNA macrohaplogroup M in India. J Genet 2009, 88:127-139. 10.1007/s12041-009-0020-3, 19417557.
73. Kashyap VK, Chattopadhyay P, Dutta R, Vasulu TS. Genetic structure and affinity among eight ethnic populations of eastern India: based on 22 polymorphic DNA loci. Am J Hum Biol 2004, 16:311-327. 10.1002/ajhb.20026, 15101056.
74. Ministry of Information & Broadcasting India Yearbook 2007, Delhi; India: Publications Division, Ministry of Information & Broadcasting, Govt. Of India, Ministry of Information & Broadcasting.
75. India [http://www.britannica.com/EBchecked/topic/285248/India].
76. Yap GC, Chee KK, Hong PY, Lay C, Satria CD. Sumadiono, Soenarto Y, Haksari EL, Aw M, Shek LP, Chua KY, Zhao Y, Leow D, Lee BW: evaluation of stool microbiota signatures in two cohorts of Asian (Singapore and Indonesia) newborns at risk of atopy. BMC Microbiol 2011, 11:193. 10.1186/1471-2180-11-193, 3171725, 21875444.
77. Nam Y-D, Jung M-J, Roh SW, Kim M-S, Bae J-W. Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing. PLoS ONE 2011, 6:e22109. 10.1371/journal.pone.0022109, 3146482, 21829445.
78. Ley RE, Hamady M, Lozupone C, Turnbaugh PJ, Ramey RR, Ramey RR, Bircher JS, Schlegel ML, Tucker TA, Schrenzel MD, Knight R, Gordon JI. Evolution of mammals and their gut microbes. Science 2008, 320:1647-1651. 10.1126/science.1155725, 2649005, 18497261.
79. Sinha R, Anderson DE, McDonald SS, Greenwald P. Cancer risk and diet in India. J Postgrad Med 2003, 49:222-228.
80. Claesson MJ, Jeffery IB, Conde S, Power SE, O'Connor EM, Cusack S, Harris HM, Coakley M, Lakshminarayanan B, O'Sullivan O, Fitzgerald GF, Deane J, O'Connor M, Harnedy N, O'Connor K, O'Mahony D, van Sinderen D, Wallace M, Brennan L, Stanton C, Marchesi JR, Fitzgerald AP, Shanahan F, Hill C, Ross RP, O'Toole PW. Gut microbiota composition correlates with diet and health in the elderly. Nature 2012, 488:178-184. 10.1038/nature11319, 22797518.
81. Ashish N. The changing popular culture of Indian food. South Asia Research 2004, 24:9-19.
82. Sofia PK, Prasad R, Vijay VK, Srivastava AK. Evaluation of antibacterial activity of Indian spices against common foodborne pathogens. Int J Food Sci Technol 2007, 42:910-915.
83. Makharia GK, Verma AK, Amarchand R, Bhatnagar S, Das P, Goswami A, Bhatia V, Ahuja V, Datta Gupta S, Anand K. Prevalence of celiac disease in the northern part of India: a community based study. J Gastroenterol Hepatol 2011, 26:894-900. 10.1111/j.1440-1746.2010.06606.x, 21182543.
84. Makharia GK, Verma AK, Amarchand R, Goswami A, Singh P, Agnihotri A, Suhail F, Krishnan A. Prevalence of irritable bowel syndrome: a community based study from northern India. J Neurogastroenterol Motil 2011, 17:1. 10.5056/jnm.2011.17.1.1, 3042210, 21369484.
85. Benjamin J, Makharia GK, Ahuja V, Kalaivani M, Joshi YK. Intestinal permeability and its association with the patient and disease characteristics in Crohn's disease. World J Gastroenterol 2008, 14:1399-1405. 10.3748/wjg.14.1399, 2693689, 18322955.
86. Misra A, Khurana L. Obesity and the metabolic syndrome in developing countries. J Clin Endocrinol Metab 2008, 93(11 Suppl 1):S9-S30.
87. Ramachandran A, Ramachandran S, Snehalatha C, Augustine C, Murugesan N, Viswanathan V, Kapur A, Williams R. Increasing expenditure on health care incurred by diabetic subjects in a developing country: a study from India. Diabetes Care 2007, 30:252-256. 10.2337/dc06-0144, 17259490.
88. Ramachandran A, Snehalatha C, Latha E, Vijay V, Viswanathan M. Rising prevalence of NIDDM in an urban population in India. Diabetologia 1997, 40:232-237. 10.1007/s001250050668, 9049486.
89. Kalra S, Unnikrishnan AG. Obesity in India: the weight of the nation. Journal of Medical Nutrition and Nutraceuticals January-June 2012, 1:37-41.
90. Gupta R, Kumar P. Global diabetes landscape - type 2 diabetes mellitus in South Asia: epidemiology, risk factors, and control. Insulin 2008, 3:78-94.
91. Weaver LJ, Narayan KM. Reconsidering the history of type 2 diabetes in India: emerging or re-emerging disease?. Natl Med J India 2008, 21:288-291.
92. Chandak GR, Janipalli CS, Bhaskar S, Kulkarni SR, Mohankrishna P, Hattersley AT, Frayling TM, Yajnik CS. Common variants in the TCF7L2 gene are strongly associated with type 2 diabetes mellitus in the Indian population. Diabetologia 2007, 50:63-67.
93. Deepa R, Sandeep S, Mohan V. Abdominal obesity, visceral fat and Type 2 diabetes - " Asian Indian Phenotype". Type 2 Diabetes in South Asians: Epidemiology, risk factors and prevention 2006, 138-152. New Delhi, India: Jaypee Brothers Medical Publishers, Mohan V, Gundu HRR.
94. Yajnik CS, Shelgikar KM, Naik SS, Sayyad MG, Raut KN, Bhat DS, Deshpande JA, Kale SD, Hockaday D. Impairment of glucosetolerance over 10 years in middle-aged normal glucose tolerant Indians. Diabetes Care 2003, 26:2212-2213. 10.2337/diacare.26.7.2212, 12832342.
95. Orenstein H, Micklin M. The Hindu joint family: the norms and the numbers. Pac Aff 39:314-325.
96. Sarem F, Sarem-Damerdji LO, Nicolas JP. Comparison of the adherence of three Lactobacillus strains to Caco-2 and Int-407 human intestinal cell lines. Lett Appl Microbiol 1996, 22:439-442. 10.1111/j.1472-765X.1996.tb01198.x, 8695070.
97. Grover S, Rashmi HM, Srivastava AK, Batish VK. Probiotics for human health -new innovations and emerging trends. Gut Pathog 2012, 4:15. 10.1186/1757-4749-4-15, 3544614, 23181893.
98. Duary RK, Rajput YS, Batish VK, Grover S. Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells. Indian J Med Res 2011, 134:664-671. 10.4103/0971-5916.90992, 3249965, 22199106.
99. Kaushik JK, Kumar A, Duary RK, Mohanty AK, Grover S, Batish VK. Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum. PLoS ONE 2009, 4:e8099. 10.1371/journal.pone.0008099, 2779496, 19956615.
100. World Health Organization World Health Statistics 2011, France: World Health Organization, World Health Organization.
101. Salyers AA, Gupta A, Wang Y. Human intestinal bacteria as reservoirs for antibiotic resistance genes. TRENDS Microbiol 2004, 12:9. 10.1016/j.tim.2003.11.002, 14700546.
102. Dethlefsen L, Huse S, Sogin ML, Relman DA. The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol 2008, 6:e280. 10.1371/journal.pbio.0060280, 2586385, 19018661.
103. Robinson Y. Antibiotic administration alters the community structure of the gastrointestinal microbiota. Gut Microbes 2010, 1:279-284. 10.4161/gmic.1.4.12614, 2954510,2954510, 20953272.
104. Ley RE, Lozupone CA, Hamady M, Knight R, Gordon JI. Worlds within worlds: evolution of the vertebrate gut microbiota. Nat Rev Microbiol 2008, 6:776-788. 10.1038/nrmicro1978, 2664199, 18794915.