Development of a fluorescent monoclonal antibody-based assay to measure the allosteric effects of synthetic peptides on self-oligomerization of AGR2 protein

Protein Science

Volumn 22, Issue 9, 2013, Pages 1266-1278

  Gray, Terry A ^a   Murray, Euan ^a,b   Nowicki, Matthew W ^a   Remnant, Lucy ^a   Scherl, Alexander ^c   Muller, Petr ^b   Vojtesek, Borek ^b   Hupp, Ted R ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b MASARYK MEMORIAL CANCER INSTITUTE   (Czech Republic)

^c UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

Allostery; Monoclonal antibody; Oligomerization; Protein interactions

Indexed keywords

MONOCLONAL ANTIBODY; PROTEIN AGR2; SYNTHETIC PEPTIDE; TUMOR PROTEIN; UNCLASSIFIED DRUG; AGR2 PROTEIN, HUMAN; CARRIER PROTEIN; DNA HELICASE; FLUORESCENT DYE; LIGAND; PROTEIN; PROTEIN BINDING; RUVBL2 PROTEIN, HUMAN;

ALLOSTERISM; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIBODY LABELING; ARTICLE; CHEMOLUMINESCENCE; DIMERIZATION; FLUORESCENCE ANALYSIS; IMMUNOASSAY; MICROTITER PLATE ASSAY; MOLECULAR LIBRARY; MOLECULAR WEIGHT; OLIGOMERIZATION; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CROSS LINKING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECTS; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; METABOLISM; MOLECULAR GENETICS; PROCEDURES; PROTEIN MULTIMERIZATION;

ALLOSTERIC REGULATION; AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; CARRIER PROTEINS; DNA HELICASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; HUMANS; LIGANDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEINS;

EID: 84892934018     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2299     Document Type: Article

References (35)

1. Morelli X, Hupp T (2012) Searching for the Holy Grail; protein-protein interaction analysis and modulation. EMBO Rep 13:877-879.
2. Thangudu R, Bryant SH, Panchenko R, Madej T (2012) Modulating protein-protein interactions with small molecules: The importance of binding hotspots. J Mol Biol 415:443-453.
3. Wright PE, Dyson HJ (1999) Intrinsically unstructured proteins: Re-assessing the protein structure-function paradigm. J Mol Biol 293:321-331.
4. Jubb H, Higueruelo AP, Winter A, Blundell TL (2012) Structural biology and drug discovery for protein-protein interactions. Trends Pharmacol Sci 33:241-248.
5. Winter A, Higueruelo AP, Marsh M, Sigurdardottir A, Pitt WR, Blundell TL (2012) Biophysical and computational fragment-based approaches to targeting protein- protein interactions: Applications in structure-guided drug discovery. Q Rev Biophys 45:383-426.
6. Smith MC, Gestwicki JE (2012) Features of protein- protein interactions that translate into potent inhibitors: Topology, surface area and affinity. Expert Rev Mol Med 14:e16.
7. Basse MJ, Betzi S, Bourgeas R, Bouzidi S, Chetrit B, Hamon V, Morelli X, Roche P (2013) 2P2Idb: A structural database dedicated to orthosteric modulation of protein-protein interactions. Nucleic Acids Res 41: D824-D827.
8. Anastasiou D, Yu Y, Israelsen WJ, Jiang JK, Boxer MB, Hong BS, Tempel W, Dimov S, Shen M, Jha A, Yang H, Mattaini KR, Metallo CM, Fiske BP, Courtney KD, Malstrom S, Khan TM, Kung C, Skoumbourdis AP, Veith H, Southall N, Walsh MJ, Brimacombe KR, Leister W, Lunt SY, Johnson ZR, Yen KE, Kunii K, Davidson SM, Christofk HR, Austin CP, Inglese J, Harris MH, Asara JM, Stephanopoulos G, Salituro FG, Jin S, Dang L, Auld DS, Park HW, Cantley LC, Thomas CJ, Vander Heiden MJ (2012) Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis. Nat Chem Biol 8:839-847.
9. Morgan HP, O'Reilly FJ, Wear MA, O'Neill JR, Fothergill-Gilmore L, Hupp T, Walkinshaw MD (2013) M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation. Proc Natl Acad Sci USA 110:5881-5886.
10. Jaffe EK, Lawrence SH (2012) Allostery and the dynamic oligomerization of porphobilinogen synthase. Arch Biochem Biophys 519:144-153.
11. Lawrence, SH, Ramirez UD, Selwood T, Stith L, Jaffe EK (2009) Allosteric inhibition of human porphobilinogen synthase. J Biol Chem 284:35807-35817.
12. Uversky VN, Oldfield CJ, Dunker AK (2008) Intrinsically disordered proteins in human diseases: Introducing the D2 concept. Annu Rev Biophys 37:215-246.
13. Tompa P (2012) On the supertertiary structure of proteins. Nat Chem Biol 8:597-600.
14. Kovacs D, Szabo B, Pancsa R, Tompa P (2012) Intrinsically disordered proteins undergo and assist folding transitions in the proteome. Arch Biochem Biophys 531:80-89.
15. Oates ME, Romero P, Ishida T, Ghalwash M, Mizianty MJ, Xue B, Dosztanyi Z, Uversky VN, Obradovic Z, Kurgan L, Dunker AK, Gough JD (2013)(2)P(2): Database of disordered protein predictions. Nucleic Acids Res 41:D508-D516.
16. Poyurovsky MV, Priest C, Kentsis A, Borden KL, Pan ZQ, Pavletich N, Prives C (2007) The Mdm2 RING domain Cterminus is required for supramolecular assembly and ubiquitin ligase activity. EMBO J 26:90-101.
17. Kostic M, Matt T, Martinez-Yamout MA, Dyson HJ, Wright PE (2006) Solution structure of the Hdm2 C2H2C4 RING, a domain critical for ubiquitination of p53. J Mol Biol 363:433-450.
18. Maslon MM, Hupp TR (2010) Drug discovery and mutant p53. Trends Cell Biol 20:542-555.
19. Robson AF, Hupp TR, Lickiss F, Ball KL, Faulds K, Graham D (2012) Nanosensing protein allostery using a bivalent mouse double minute two (MDM2) assay. Proc Natl Acad Sci USA 109:8073-8078.
20. Murray E, McKenna EO, Burch LR, Dillon J, Langridge-Smith P, Kolch W, Pitt, A, Hupp TR (2007) Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2. Biochemistry 46:13742-13751.
21. Chevet E, Fessart D, Delom F, Mulot A, Vojtesek B, Hrstka R, Murray E, Gray, T, Hupp T (2013) Emerging roles for the pro-oncogenic anterior gradient-2 in cancer development. Oncogene 32:2499-2509
22. Fourtouna A, Murray E, Nicholson J, Maslon MM, Pang L, Dryden DTF, Hupp TR (2009) The anterior gradient-2 pathway as a model for developing peptideaptamer anti-cancer drug leads that stimulate p53 function. Curr Chem Biol 3:124-137.
23. Patel P, Clarke, C, Barraclough DL, Jowitt TA, Rudland PS, Barraclough R, Lian, LY (2012) Metastasis- promoting anterior gradient 2 protein has a dimeric thioredoxin fold structure and a role in cell adhesion. J Mol Biol 425:929-943.
24. Hengel SM, Murray E, Langdon S, Hayward L, O'Donoghue J, Panchaud A, Hupp T, Goodlett DR (2011) Data-independent proteomic screen identifies novel tamoxifen agonist that mediates drug resistance. J Proteome Res 10:4567-4578.
25. Gray TA, MacLaine NJ, Michie CO, Bouchalova P, Murray E, Howie J, Hrstka R, Maslon MM, Nenutil R, Vojtesek B, Langdon S, Hayward L, Gourley C, Hupp TR (2012) Anterior gradient-3: A novel biomarker for ovarian cancer that mediates cisplatin resistance in xenograft models. J Immunol Methods 378:20-32.
26. Maslon MM, Hrstka R, Vojtesek B, Hupp TR (2010) A divergent substrate-binding loop within the prooncogenic protein anterior gradient-2 forms a docking site for Reptin. J Mol Biol 404:418-438.
27. Higa A, Mulot A, Delom F, Bouchecareilh M, Nguyen DT, Boismenu D, Wise MJ, Chevet E (2011) Role of pro-oncogenic protein disulfide isomerase (PDI) family member anterior gradient 2 (AGR2) in the control of endoplasmic reticulum homeostasis. J Biol Chem 286: 44855-44868.
28. Gupta A, Dong A, Lowe AW (2012) AGR2 gene function requires a unique endoplasmic reticulum localization motif. J Biol Chem 287:4773-4782.
29. Pohler E, Craig AL, Cotton J, Lawrie, L, Dillon JF, Ross P, Kernohan N, Hupp TR (2004) The Barrett's antigen anterior gradient-2 silences the p53 transcriptional response to DNA damage. Mol Cell Proteomics 3:534-547.
30. Bouchecareilh M, Higa A, Fribourg S, Moenner M, Chevet E (2011) Peptides derived from the bifunctional kinase/RNase enzyme IRE1alpha modulate IRE1alpha activity and protect cells from endoplasmic reticulum stress. FASEB J 25:3115-3129.
31. Nicholson J, Neelagandan K, Huart AS, Ball K, Molloy MP, Hupp TR (2012) An iTRAQ proteomics screen reveals the effects of the MDM2 binding ligand Nutlin-3 on cellular proteostasis. J Proteome Res 11: 5464-5478.
32. Liu H, Naismith JH (2009) A simple and efficient expression and purification system using two newly constructed vectors. Protein Expr Purif 63:102-111.
33. Singh P, Shaffer SA, Scherl A, Holman C, Pfuetzner RA, Larson Freeman TJ, Miller SI, Hernandez P, Appel RD, Goodlett DR (2008) Characterization of protein cross-links via mass spectrometry and an openmodification search strategy. Anal Chem 80:8799-8806.
34. Tsutsui Y, Wintrode PL (2007) Hydrogen/deuterium exchange-mass spectrometry: A powerful tool for probing protein structure, dynamics and interactions. Curr Med Chem 14:2344-2358.
35. Marin M, Thallmair V, Ott T (2012) The intrinsically disordered N-terminal region of AtREM1.3 remorin protein mediates protein-protein interactions. J Biol Chem 287:39982-39991.