Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system

Biotechnology and Bioengineering

Volumn 111, Issue 3, 2014, Pages 586-596

  Nakamura, Yasuyuki ^a   Takemoto, Norika ^a   Ishii, Jun ^a   Kondo, Akihiko ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

Dimerization; G protein signaling; G protein coupled receptor; Saccharomyces cerevisiae; Split ubiquitin yeast two hybrid system

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; G PROTEIN; G PROTEIN COUPLED RECEPTORS; PHYSIOLOGICAL DIVERSITY; SIMULTANEOUS DETECTION; SIMULTANEOUS METHODS; SOMATOSTATIN RECEPTORS; YEAST TWO-HYBRID SYSTEM;

ASSAYS; COLOR; DIMERIZATION; DIMERS; FLUORESCENCE; HYBRID SYSTEMS; LIGANDS; PROTEINS; SIGNALING; YEAST;

CELL SIGNALING;

ENHANCED GREEN FLUORESCENT PROTEIN; G PROTEIN COUPLED RECEPTOR; PHEROMONE RECEPTOR; RED FLUORESCENT PROTEIN; SOMATOSTATIN; SOMATOSTATIN RECEPTOR 5; UBIQUITIN;

ARTICLE; BIOLOGICAL FUNCTIONS; CONTROLLED STUDY; DIMERIZATION; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; MUTATIONAL ANALYSIS; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; REPORTER GENE; SIGNAL TRANSDUCTION; TWO HYBRID SYSTEM;

SACCHAROMYCES CEREVISIAE;

COLOR; FLUORESCENCE; GENES, REPORTER; GENETICS, MICROBIAL; GREEN FLUORESCENT PROTEINS; GTP-BINDING PROTEINS; LUMINESCENT PROTEINS; MOLECULAR BIOLOGY; PROTEIN MULTIMERIZATION; RECEPTORS, G-PROTEIN-COUPLED; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; STAINING AND LABELING;

EID: 84892793340     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25125     Document Type: Article

References (53)

1. AbdAlla S, Lother H, Quitterer U. 2000. AT1-receptor heterodimers show enhanced G-protein activation and altered receptor sequestration. Nature 407(6800):94-98.
2. Akada R, Kitagawa T, Kaneko S, Toyonaga D, Ito S, Kakihara Y, Hoshida H, Morimura S, Kondo A, Kida K. 2006. PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae. Yeast 23(5):399-405.
3. Benkirane M, Jin DY, Chun RF, Koup RA, Jeang KT. 1997. Mechanism of transdominant inhibition of CCR5-mediated HIV-1 infection by ccr5delta32. J Biol Chem 272(49):30603-30606.
4. Ben-Shlomo A, Melmed S. 2008. Somatostatin agonists for treatment of acromegaly. Mol Cell Endocrinol 286(1-2):192-198.
5. Bockaert J, Perroy J, Bécamel C, Marin P, Fagni L. 2010. GPCR interacting proteins (GIPs) in the nervous system: Roles in physiology and pathologies. Annu Rev Pharmacol Toxicol 50:89-109.
6. Brachmann CB, Davies A, Cost GJ, Caputo E, Li J, Hieter P, Boeke JD. 1998. Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14(2):115-132.
7. Cvejic S, Devi LA. 1997. Dimerization of the delta opioid receptor: Implication for a role in receptor internalization. J Biol Chem 272(43):26959-26964.
8. Dowell SJ, Brown AJ. 2002. Yeast assays for G-protein-coupled receptors. Receptors Channels 8(5-6):343-352.
9. Drews J. 2000. Drug discovery: A historical perspective. Science 287(5460):1960-1964.
10. Ferré S, Franco R. 2010. Oligomerization of G-protein-coupled receptors: A reality. Curr Opin Pharmacol 10(1):1-5.
11. Fredriksson R, Lagerström MC, Lundin LG, Schiöth HB. 2003. The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints. Mol Pharmacol 63:1256-1272.
12. Fukuda N, Ishii J, Kaishima M, Kondo A. 2011. Amplification of agonist stimulation of human G-protein-coupled receptor signaling in yeast. Anal Biochem 417(2):182-187.
13. Fukutani Y, Nakamura T, Yorozu M, Ishii J, Kondo A, Yohda M. 2012. The N-terminal replacement of an olfactory receptor for the development of a yeast-based biomimetic odor sensor. Biotechnol Bioeng 109(1):205-212.
14. Gehret AU, Bajaj A, Naider F, Dumont ME. 2006. Oligomerization of the yeast alpha-factor receptor: Implications for dominant negative effects of mutant receptors. J Biol Chem 281(30):20698-20714.
15. Gietz D, St Jean A, Woods RA, Schiestl RH. 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20(6):1425.
16. Grant M, Alturaihi H, Jaquet P, Collier B, Kumar U. 2008. Cell growth inhibition and functioning of human somatostatin receptor type 2 are modulated by receptor heterodimerization. Mol Endocrinol 22(10):2278-2292.
17. Grosse R, Schöneberg T, Schultz G, Gudermann T. 1997. Inhibition of gonadotropin-releasing hormone receptor signaling by expression of a splice variant of the human receptor. Mol Endocrinol 11(9):1305-1318.
18. Gudermann T, Nürnberg B, Schultz G. 1995. Receptors and G proteins as primary components of transmembrane signal transduction. Part 1. G-protein-coupled receptors: Structure and function. J Mol Med 73(2):51-63.
19. Hara K, Ono T, Kuroda K, Ueda M. 2012. Membrane-displayed peptide ligand activates the pheromone response pathway in Saccharomyces cerevisiae. J Biochem 151(5):551-557.
20. Hebert TE, Moffett S, Morello JP, Loisel TP, Bichet DG, Barret C, Bouvier M. 1996. A peptide derived from a beta2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation. J Biol Chem 271(27):16384-16392.
21. Iguchi Y, Ishii J, Nakayama H, Ishikura A, Izawa K, Tanaka T, Ogino C, Kondo A. 2010. Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast. J Biochem 147(6):875-884.
22. Ishii J, Matsumura S, Kimura S, Tatematsu K, Kuroda S, Fukuda H, Kondo A. 2006. Quantitative and dynamic analyses of G protein-coupled receptor signaling in yeast using Fus1, enhanced green fluorescence protein (EGFP), and His3 fusion protein. Biotechnol Prog 22(4):954-960.
23. Ishii J, Izawa K, Matsumura S, Wakamura K, Tanino T, Tanaka T, Ogino C, Fukuda H, Kondo A. 2009. A simple and immediate method for simultaneously evaluating expression level and plasmid maintenance in yeast. J Biochem 145(6):701-708.
24. Ishii J, Fukuda N, Tanaka T, Ogino C, Kondo A. 2010. Protein-protein interactions and selection: Yeast-based approaches that exploit guanine nucleotide-binding protein signaling. FEBS J 277(9):1982-1995.
25. Ishii J, Moriguchi M, Hara KY, Shibasaki S, Fukuda H, Kondo A. 2012a. Improved identification of agonist-mediated Gα(i)-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry. Anal Biochem 426(2):129-133.
26. Ishii J, Yoshimoto N, Tatematsu K, Kuroda S, Ogino C, Fukuda H, Kondo A. 2012b. Cell wall trapping of autocrine peptides for human G-protein-coupled receptors on the yeast cell surface. PLoS ONE 7(5):e37136.
27. Iyer K, Bürkle L, Auerbach D, Thaminy S, Dinkel M, Engels K, Stagljar I. 2005. Utilizing the split-ubiquitin membrane yeast two-hybrid system to identify protein-protein interactions of integral membrane proteins. Sci STKE 2005(275):pl3.
28. Jordan BA, Devi LA. 1999. G-protein-coupled receptor heterodimerization modulates receptor function. Nature 399(6737):697-700.
29. Jordan BA, Trapaidze N, Gomes I, Nivarthi R, Devi LA. 2001. Oligomerization of opioid receptors with beta 2-adrenergic receptors: A role in trafficking and mitogen-activated protein kinase activation. Proc Natl Acad Sci USA 98(1):343-348.
30. Kittanakom S, Chuk M, Wong V, Snyder J, Edmonds D, Lydakis A, Zhang Z, Auerbach D, Stagljar I. 2009. Analysis of membrane protein complexes using the split-ubiquitin membrane yeast two-hybrid (MYTH) system. Methods Mol Biol 548:247-271.
31. Ladds G, Goddard A, Davey J. 2005. Functional analysis of heterologous GPCR signalling pathways in yeast. Trends Biotechnol 23(7):367-373.
32. Lamberts SW, de Herder WW, Krenning EP, Reubi JC. 1994. A role of (labeled) somatostatin analogs in the differential diagnosis and treatment of Cushing's syndrome. J Clin Endocrinol Metab 78(1):17-19.
33. Lefkowitz RJ, Shenoy SK. 2005. Transduction of receptor signals by beta-arrestins. Science 308(5721):512-517.
34. Lybarger L, Dempsey D, Patterson GH, Piston DW, Kain SR, Chervenak R. 1998. Dual-color flow cytometric detection of fluorescent proteins using single-laser (488-nm) excitation. Cytometry 31(3):147-152.
35. Margeta-Mitrovic M, Jan YN, Jan LY. 2000. A trafficking checkpoint controls GABA(B) receptor heterodimerization. Neuron 27(1):97-106.
36. Mijares A, Lebesque D, Wallukat G, Hoebeke J. 2000. From agonist to antagonist: Fab fragments of an agonist-like monoclonal anti-beta(2)-adrenoceptor antibody behave as antagonists. Mol Pharmacol 58(2):373-379.
37. Minic J, Sautel M, Salesse R, Pajot-Augy E. 2005. Yeast system as a screening tool for pharmacological assessment of G protein coupled receptors. Curr Med Chem 12(8):961-969.
38. Nakamura Y, Ishii J, Kondo A. 2013. Rapid, facile detection of heterodimer partners for target human G-protein-coupled receptors using a modified split-ubiquitin membrane yeast two-hybrid system. PLoS ONE 8(6):e66793.
39. Overington JP, Al-Lazikani B, Hopkins AL. 2006. How many drug targets are there? Nat Rev Drug Discov 5(12):993-996.
40. Overton MC, Blumer KJ. 2002. The extracellular N-terminal domain and transmembrane domains 1 and 2 mediate oligomerization of a yeast G protein-coupled receptor. J Biol Chem 277(44):41463-41472.
41. Overton MC, Chinault SL, Blumer KJ. 2003. Oligomerization, biogenesis, and signaling is promoted by a glycophorin A-like dimerization motif in transmembrane domain 1 of a yeast G protein-coupled receptor. J Biol Chem 278(49):49369-49377.
42. Potter LT, Ballesteros LA, Bichajian LH, Ferrendelli CA, Fisher A, Hanchett HE, Zhang R. 1991. Evidence of paired M2 muscarinic receptors. Mol Pharmacol 39(2):211-221.
43. Rasmussen SG, Choi HJ, Rosenbaum DM, Kobilka TS, Thian FS, Edwards PC, Burghammer M, Ratnala VR, Sanishvili R, Fischetti RF, Schertler GF, Weis WI, Kobilka BK. 2007. Crystal structure of the human beta2 adrenergic G-protein-coupled receptor. Nature 450(7168):383-387.
44. Ritter SL, Hall RA. 2009. Fine-tuning of GPCR activity by receptor-interacting proteins. Nat Rev Mol Cell Biol 10(12):819-830.
45. Saito T, Iwata N, Tsubuki S, Takaki Y, Takano J, Huang SM, Suemoto T, Higuchi M, Saido TC. 2005. Somatostatin regulates brain amyloid beta peptide Abeta42 through modulation of proteolytic degradation. Nat Med 11(4):434-439.
46. Somvanshi RK, Chaudhari N, Qiu X, Kumar U. 2011. Heterodimerization of beta2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway. J Mol Signal 6:9.
47. Stagljar I, Korostensky C, Johnsson N, te Heesen S. 1998. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc Natl Acad Sci USA 95(9):5187-5192.
48. Stewart GD, Valant C, Dowell SJ, Mijaljica D, Devenish RJ, Scammells PJ, Sexton PM, Christopoulos A. 2009. Determination of adenosine A1 receptor agonist and antagonist pharmacology using Saccharomyces cerevisiae: Implications for ligand screening and functional selectivity. J Pharmacol Exp Ther 331(1):277-286.
49. Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BS. 2009. A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling. Biochemistry 48(35):8279-8281.
50. Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C, Kondo A. 2010. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signaling. J Biochem 147(6):867-873.
51. Wang HX, Konopka JB. 2009. Identification of amino acids at two dimer interface regions of the alpha-factor receptor (Ste2). Biochemistry 48(30):7132-7139.
52. Winzeler EA, Shoemaker DD, Astromoff A, Liang H, Anderson K, Andre B, Bangham R, Benito R, Boeke JD, Bussey H, Chu AM, Connelly C, Davis K, Dietrich F, Dow SW, El Bakkoury M, Foury F, Friend SH, Gentalen E, Giaever G, Hegemann JH, Jones T, Laub M, Liao H, Liebundguth N, Lockhart DJ, Lucau-Danila A, Lussier M, M'Rabet N, Menard P, Mittmann M, Pai C, Rebischung C, Revuelta JL, Riles L, Roberts CJ, Ross-MacDonald P, Scherens B, Snyder M, Sookhai-Mahadeo S, Storms RK, Véronneau S, Voet M, Volckaert G, Ward TR, Wysocki R, Yen GS, Yu K, Zimmermann K, Philippsen P, Johnston M, Davis RW. 1999. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285(5429):901-906.
53. Yesilaltay A, Jenness DD. 2000. Homo-oligomeric complexes of the yeast alpha-factor pheromone receptor are functional units of endocytosis. Mol Biol Cell 11(9):2873-2884.