Dual-color flow cytometric detection of fluorescent proteins using single-laser (488-nm) excitation

Cytometry

Volumn 31, Issue 3, 1998, Pages 147-152

  Lybarger, Lonnie ^a   Dempsey, Deborah ^a   Patterson, George H ^b   Piston, David W ^b   Kain, Steven R ^c   Chervenak, Robert ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c CLONTECH Laboratories Inc   (United States)

Author keywords

GFP; Reporter gene; Retrovirus vector; YFP

Indexed keywords

GREEN FLUORESCENT PROTEIN; VIRUS VECTOR;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; EXCITATION; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN LOCALIZATION; REPORTER GENE; RETROVIRUS; TARGET CELL;

3T3 CELLS; ANIMALS; BACTERIAL PROTEINS; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LASERS; LUMINESCENT PROTEINS; MICE; RECOMBINANT FUSION PROTEINS; RETROVIRIDAE; TRANSFECTION;

ANIMALIA; MURINAE; UNIDENTIFIED RETROVIRUS;

EID: 0032030837     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19980301)31:3<147::AID-CYTO1>3.0.CO;2-I     Document Type: Article

References (19)

1. Anderson M, Tjioe I, Lorincz M, Parks D, Herzenberg L, Nolan G, Herzenberg L: Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. Proc Natl Acad Sci 93:8508-8511, 1996.
2. Bierhuizen M, Westerman Y, Visser T, Wognum A, Wagemaker G: Green fluorescent protein variants as markers of retroviral-mediated gene transfer in primary hematopoietic cells and cell lines. Biochem Biophys Res Commun 234:371-375, 1997.
3. Chalfie M, Tu Y, Euskirchen G, Ward W: Green fluorescent protein as a marker for gene expression. Science 263:802-805, 1994.
4. Cormack B, Valdivia R, Falkow S: FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38, 1996.
5. Delgrave S, Hawtin R, Silva C, Yang M, Youvan D: Red-shifted excitation mutants of the green fluorescent protein. BioTechnology 13:151-154, 1995.
6. Heim R, Cubitt A, Tsien R: Improved green fluorescence. Nature 373:663-664, 1995.
7. Inouye S, Tsuji F: Aequorea green fluorescent protein: Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett 341:277-280, 1994.
8. Jainchill J, Aaronson S, Todaro G: Murine sarcoma and leukemia viruses: Assay using clonal lines of contact-inhibited mouse cells. J Virol 4:549-553, 1969
9. Lybarger L, Dempsey D, Franek K, Chervenak R: Rapid generation and flow cytometric analysis of stable GFP-expressing cells. Cytometry 25:211-220, 1996.
10. Markowitz D, Goff S, Bank A: A safe packaging line for gene transfer: Separating viral genes of two different plasmids. J Virol 62:1120-1124, 1988.
11. Morgenstern J, Land H: Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res 18:3587-3596, 1990.
12. Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y: "Green Mice" as a source of ubiquitous green cells. FEBS Lett 407:313-319, 1997.
13. Ormö M, Cubitt A, Kallio K, Gross L, Tsien R, Remington S: Crystal structure of the Aequorea victoria green fluorescent protein. Science 273:1392-1395, 1996.
14. Patterson G, Knobel S, Sharif W, Kain S, Piston D: Use of the green fluorescent protein (GFP) and its mutants in quantitative fluorescence microscopy. Biophys J 73:2782-2790, 1997.
15. Prasher D, Eckenrode V, Ward W, Prendergast F, Cormier M: Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229-233, 1992.
16. Ropp J. Donahue C, Wolfgang-Kimball D, Hooley J, Chin J, Hoffman R, Cuthbertson R, Bauer K: Aequorea green fluorescent protein analysis by flow cytometry. Cytometry 21:309-317, 1995.
17. Ropp J, Donahue C, Wolfgang-Kimball D, Hooley J, Chin J, Cuthbertson R, Bauer K: Aequorea green fluorescent protein: simultaneous analysis of wild-type and blue-fluorescing mutant by flow cytometry. Cytometry 24:284-288, 1996.
18. Shimomura O, Johnson F, Saiga Y: Extraction, purification and properties of Aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol 59:223-227, 1962.
19. Yang T-T, Cheng L, Kain S: Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucleic Acids Res 24:4592-4593, 1996.