Efficient designer nuclease-based homologous recombination enables direct pcr screening for footprintless targeted human pluripotent stem cells

Stem Cell Reports

Volumn 2, Issue 1, 2014, Pages 107-118

  Merkert, Sylvia ^a   Wunderlich, Stephanie ^a   Bednarski, Christien ^a,b   Beier, Jennifer ^a   Haase, Alexandra ^a   Dreyer, Anne Kathrin ^a   Schwanke, Kristin ^a   Meyer, Johann ^a   Göhring, Gudrun ^a   Cathomen, Toni ^a,b   Martin, Ulrich ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; NUCLEASE; OLIGODEOXYNUCLEOTIDE; ENDONUCLEASE; OLIGODEOXYRIBONUCLEOTIDE;

ARTICLE; CELL CLONE; EX VIVO GENE TRANSFER; FLOW CYTOMETRY; GENE MUTATION; GENE TARGETING; GENE THERAPY; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; PLASMID; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPORTER GENE; TRANSGENE; CELL CULTURE; CYTOLOGY; DNA END JOINING REPAIR; GENE INACTIVATION; GENE LOCUS; METABOLISM;

CELLS, CULTURED; DNA END-JOINING REPAIR; ENDONUCLEASES; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENETIC LOCI; HOMOLOGOUS RECOMBINATION; HUMANS; INDUCED PLURIPOTENT STEM CELLS; OLIGODEOXYRIBONUCLEOTIDES; PLURIPOTENT STEM CELLS; POLYMERASE CHAIN REACTION;

EID: 84892584919     PISSN: 22136711     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.stemcr.2013.12.003     Document Type: Article

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