Cytokine production using membrane adsorbers: Human basic fibroblast growth factor produced by Escherichia coli

Engineering in Life Sciences

Volumn 12, Issue 1, 2012, Pages 29-38

  Chen, Ran ^a   John, Jinu ^a   Lavrentieva, Antonina ^a   Müller, Susann ^b   Tomala, Magda ^a   Zhao, Yangxi ^a   Zweigerdt, Robert ^b   Beutel, Sascha ^a   Hitzmann, Bernd ^a   Kasper, Cornelia ^a   Martin, Ulrich ^b   Rinas, Ursula ^a,c   Stahl, Frank ^a   Scheper, Thomas ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Basic fibroblast growth factor; Escherichia coli BL21; Membrane adsorber technology; Pluripotent stem cell culture

Indexed keywords

ADSORBERS; ANION EXCHANGE MEMBRANE; BASIC FIBROBLAST GROWTH FACTOR; CATION EXCHANGE MEMBRANES; CELLULAR PROCESS; CULTIVATION PROCESS; CULTURE BROTHS; CYTOKINE PRODUCTION; CYTOKINES; DRY CELLS; E. COLI; EFFICIENT PROCESS; EMBRYONIC STEM CELLS; EXPRESSION LEVELS; FED-BATCH CULTIVATION; HIGH PRICE; IN-VITRO; PLURIPOTENT; PLURIPOTENT STEM CELLS; POLISHING STEPS; PURIFIED PROTEIN; RECOMBINANT ESCHERICHIA COLI; STEM CELL RESEARCH;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ANIMAL CELL CULTURE; COMPUTER SOFTWARE REUSABILITY; ESCHERICHIA COLI; FIBROBLASTS; GEL PERMEATION CHROMATOGRAPHY; MEMBRANE TECHNOLOGY; PEPTIDES; STEM CELLS;

PURIFICATION;

CYTOKINE; DNA; ENDOTOXIN; FIBROBLAST GROWTH FACTOR 2; HEPARIN; SEPHAROSE;

ADSORPTION; CHROMATOGRAPHY; COLIFORM BACTERIUM; CYTOLOGY; HOMINID; INDUSTRIAL PRODUCTION; MEMBRANE; MICROBIAL ACTIVITY; PRODUCTION COST; PROTEIN; PURIFICATION; RESEARCH WORK;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; COST BENEFIT ANALYSIS; CYTOKINE PRODUCTION; EMBRYONIC STEM CELL; ESCHERICHIA COLI; FED BATCH CULTURE; HUMAN; HUMAN CELL; ION EXCHANGE CHROMATOGRAPHY; MEMBRANE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ESCHERICHIA COLI; PRIMATES;

EID: 84863011321     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201100045     Document Type: Article

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