Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Franko, Andras ^a   Baris, Olivier R ^b   Bergschneider, Eva ^c   Von Toerne, Christine ^a   Hauck, Stefanie M ^a   Aichler, Michaela ^a   Walch, Axel K ^a   Wurst, Wolfgang ^d,e,f   Wiesner, Rudolf J ^b   Johnston, Ian C D ^c   De Angelis, Martin Hrabě ^a,d,g  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b UNIVERSITY OF COLOGNE   (Germany)

^c MILTENYI BIOTEC GMBH   (Germany)

^d TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^e MAX PLANCK INSTITUTE OF PSYCHIATRY   (Germany)

^f GERMAN CENTER FOR NEURODEGENERATIVE DISEASES DZNE   (Germany)

^g GERMAN CENTER FOR DIABETES RESEARCH DZD   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MITOCHONDRIAL PROTEIN; PROTEIN ANTIBODY; TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 22; TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 22 ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN MITOCHONDRION; CELL ISOLATION; CENTRIFUGATION; ELECTRODE; LIVER MITOCHONDRION; MAGNET; MAGNETIC BEAD; MAGNETIC SEPARATION; MOUSE; MUSCLE MITOCHONDRION; NONHUMAN; OXYGEN CONSUMPTION; PROTEOMICS; REPRODUCIBILITY; TRANSMISSION ELECTRON MICROSCOPY; WESTERN BLOTTING;

ANIMALS; AUTOMATION; CELL FRACTIONATION; CENTRIFUGATION; MAGNETIC PHENOMENA; MICE; MICROSPHERES; MITOCHONDRIA; MITOCHONDRIA, LIVER; MITOCHONDRIA, MUSCLE; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; ORGAN SPECIFICITY; OXYGEN CONSUMPTION; SUBCELLULAR FRACTIONS;

EID: 84892568694     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0082392     Document Type: Article

References (27)

1. Vafai SB, Mootha VK (2012) Mitochondrial disorders as windows into an ancient organelle. Nature 491: 374-383.
2. Zsurka G, Kunz WS (2010) Mitochondrial dysfunction in neurological disorders with epileptic phenotypes. J Bioenerg Biomembr 42: 443-448.
3. Ekstrand MI, Terzioglu M, Galter D, Zhu S, Hofstetter C, et al. (2007) Progressive parkinsonism in mice with respiratory-chain-deficient dopamine neurons. Proc Natl Acad Sci U S A 104: 1325-1330.
4. Park CB, Larsson NG (2011) Mitochondrial DNA mutations in disease and aging. J Cell Biol 193: 809-818.
5. Szendroedi J, Schmid AI, Chmelik M, Toth C, Brehm A, et al. (2007) Muscle mitochondrial ATP synthesis and glucose transport/phosphorylation in type 2 diabetes. PLoS Med 4: e154.
6. Szendroedi J, Chmelik M, Schmid AI, Nowotny P, Brehm A, et al. (2009) Abnormal hepatic energy homeostasis in type 2 diabetes. Hepatology 50: 1079-1086.
7. Bonnard C, Durand A, Peyrol S, Chanseaume E, Chauvin MA, et al. (2008) Mitochondrial dysfunction results from oxidative stress in the skeletal muscle of diet-induced insulin-resistant mice. J Clin Invest 118: 789-800.
8. Franko A, von Kleist-Retzow JC, Bose M, Sanchez-Lasheras C, Brodesser S, et al. (2012) Complete failure of insulin-transmitted signaling, but not obesityinduced insulin resistance, impairs respiratory chain function in muscle. J Mol Med (Berl) 90: 1145-1160.
9. Horan MP, Pichaud N, Ballard JW (2012) Review: quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67: 1022-1035.
10. Rogers GW, Brand MD, Petrosyan S, Ashok D, Elorza AA, et al. (2011) High throughput microplate respiratory measurements using minimal quantities of isolated mitochondria. PLoS One 6: e21746.
11. Sims NR (1990) Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation. J Neurochem 55: 698-707.
12. Sims NR, Anderson MF (2008) Isolation of mitochondria from rat brain using Percoll density gradient centrifugation. Nat Protoc 3: 1228-1239.
13. Hartwig S, Feckler C, Lehr S, Wallbrecht K, Wolgast H, et al. (2009) A critical comparison between two classical and a kit-based method for mitochondria isolation. Proteomics 9: 3209-3214.
14. Frezza C, Cipolat S, Scorrano L (2007) Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nat Protoc 2: 287-295.
15. Fernandez-Vizarra E, Ferrin G, Perez-Martos A, Fernandez-Silva P, Zeviani M, et al. (2010) Isolation of mitochondria for biogenetical studies: An update. Mitochondrion 10: 253-262.
16. Gross VS, Greenberg HK, Baranov SV, Carlson GM, Stavrovskaya IG, et al. (2011) Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization. Anal Biochem 418: 213-223.
17. Hornig-Do HT, Gunther G, Bust M, Lehnartz P, Bosio A, et al. (2009) Isolation of functional pure mitochondria by superparamagnetic microbeads. Anal Biochem 389: 1-5.
18. Rustin P, Chretien D, Bourgeron T, Gerard B, Rotig A, et al. (1994) Biochemical and molecular investigations in respiratory chain deficiencies. Clin Chim Acta 228: 35-51.
19. Merl J, Ueffing M, Hauck SM, von Toerne C (2012) Direct comparison of MSbased label-free and SILAC quantitative proteome profiling strategies in primary retinal Muller cells. Proteomics 12: 1902-1911.
20. von Toerne C, Kahle M, Schafer A, Ispiryan R, Blindert M, et al. (2013) Apoe, Mbl2, and Psp plasma protein levels correlate with diabetic phenotype in NZO mice-an optimized rapid workflow for SRM-based quantification. J Proteome Res 12: 1331-1343.
21. Hauck SM, Dietter J, Kramer RL, Hofmaier F, Zipplies JK, et al. (2010) Deciphering membrane-associated molecular processes in target tissue of autoimmune uveitis by label-free quantitative mass spectrometry. Mol Cell Proteomics 9: 2292-2305.
22. Brosch M, Yu L, Hubbard T, Choudhary J (2009) Accurate and sensitive peptide identification with Mascot Percolator. J Proteome Res 8: 3176-3181.
23. Rainer J, Sanchez-Cabo F, Stocker G, Sturn A, Trajanoski Z (2006) CARMAweb: comprehensive R- and bioconductor-based web service for microarray data analysis. Nucleic Acids Res 34: W498-503.
24. Laurindo FR, Pescatore LA, Fernandes Dde C (2012) Protein disulfide isomerase in redox cell signaling and homeostasis. Free Radic Biol Med 52: 1954-1969.
25. Pagliarini DJ, Calvo SE, Chang B, Sheth SA, Vafai SB, et al. (2008) A mitochondrial protein compendium elucidates complex I disease biology. Cell 134: 112-123.
26. Rasmussen HN, Andersen AJ, Rasmussen UF (1997) Optimization of preparation of mitochondria from 25-100 mg skeletal muscle. Anal Biochem 252: 153-159.
27. Kristian T, Hopkins IB, McKenna MC, Fiskum G (2006) Isolation of mitochondria with high respiratory control from primary cultures of neurons and astrocytes using nitrogen cavitation. J Neurosci Methods 152: 136-143.