Isolation of mitochondria with high respiratory control from primary cultures of neurons and astrocytes using nitrogen cavitation

Journal of Neuroscience Methods

Volumn 152, Issue 1-2, 2006, Pages 136-143

  Kristián, Tibor ^a   Hopkins, Irene B ^a   McKenna, Mary C ^a   Fiskum, Gary ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Astrocytes; Isolation; Mitochondria; Neurons; Nitrogen cavitation; Peripheral benzodiazepine receptor; Primary cell culture

Indexed keywords

NITROGEN;

ANIMAL CELL; ARTICLE; ASTROCYTE; BRAIN MITOCHONDRION; BRAIN TISSUE; CELL DISRUPTION; CELL FREE SYSTEM; CELL ISOLATION; CELL MEMBRANE PERMEABILITY; CELL SUSPENSION; CONTROLLED STUDY; EMBRYO; MITOCHONDRIAL RESPIRATION; NERVE CELL; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; ASTROCYTES; BLOTTING, WESTERN; CELLS, CULTURED; CEREBRAL CORTEX; MICROSCOPY, ELECTRON; MITOCHONDRIA; NERVE TISSUE PROTEINS; NEURONS; NITROGEN; OXYGEN CONSUMPTION; RATS; RECEPTORS, GABA-A;

EID: 33644781684     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2005.08.018     Document Type: Article

References (33)

1. S. Adachi, A.R. Cross, B.M. Babior, and R.A. Gottlieb Bcl-2 and the outer mitochondrial membrane in the inactivation of cytochrome c during Fas-mediated apoptosis J Biol Chem 272 1997 21878 21882
2. S. Adachi, R.A. Gottlieb, and B.M. Babior Lack of release of cytochrome C from mitochondria into cytosol early in the course of Fas-mediated apoptosis of Jurkat cells J Biol Chem 273 1998 19892 19894
3. A. Almeida, and J.M. Medina Different mitochondrial coupling in neurons and in astrocytes in primary culture Biochem Soc Trans 25 1997 410S
4. A. Almeida, and J.M. Medina Isolation and characterization of tightly coupled mitochondria from neurons and astrocytes in primary culture Brain Res 764 1997 167 172
5. N. Auestad, R.A. Korsak, J.W. Morrow, and J. Edmond Fatty acid oxidation and ketogenesis by astrocytes in primary culture J Neurochem 56 1991 1376 1386
6. R.B. Banati, R. Myers, and G.W. Kreutzberg PK ('peripheral benzodiazepine')-binding sites in the CNS indicate early and discrete brain lesions: microautoradiographic detection of [3H]PK11195 binding to activated microglia J Neurocytol 26 1997 77 82
7. J.P. Bolanos, S.J. Heales, J.M. Land, and J.B. Clark Effect of peroxynitrite on the mitochondrial respiratory chain: differential susceptibility of neurones and astrocytes in primary culture J Neurochem 64 1995 1965 1972
8. J. Booher, and M. Sensenbrenner Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures Neurobiology 2 1972 97 105
9. M.R. Brown, J.W. Geddes, and P.G. Sullivan Brain region-specific, age-related, alterations in mitochondrial responses to elevated calcium J Bioenerg Biomembr 36 2004 401 406
10. 2+] Biochemistry 35 1996 8172 8180
11. E.L. Conway, A.L. Gundlach, and J.A. Craven Temporal changes in glial fibrillary acidic protein messenger RNA and [3H]PK11195 binding in relation to imidazoline-I2-receptor and alpha 2-adrenoceptor binding in the hippocampus following transient global forebrain ischaemia in the rat Neuroscience 82 1998 805 817
12. P.M. Elias, J. Goerke, D.S. Friend, and B.E. Brown Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes J Cell Biol 78 1978 577 596
13. R.A. Gottlieb, and S. Adachi Nitrogen cavitation for cell disruption to obtain mitochondria from cultured cells Methods Enzymol 322 2000 213 221
14. A.R. Jayakumar, K.S. Panickar, and M.D. Norenberg Effect on free radical generation by ligands of the peripheral benzodiazepine receptor in cultured neural cells J Neurochem 83 2002 1226 1234
15. A. Krippner, A. Matsuno-Yagi, R.A. Gottlieb, and B.M. Babior Loss of function of cytochrome c in Jurkat cells undergoing fas-mediated apoptosis J Biol Chem 271 1996 21629 21636
16. T. Kristian, J. Gertsch, T.E. Bates, and B.K. Siesjo Characteristics of the calcium-triggered mitochondrial permeability transition in nonsynaptic brain mitochondria: effect of cyclosporin A and ubiquinone O J Neurochem 74 2000 1999 2009
17. T. Kristian, T.M. Weatherby, T.E. Bates, and G. Fiskum Heterogeneity of the calcium-induced permeability transition in isolated non-synaptic brain mitochondria J Neurochem 83 2002 1297 1308
18. J.C. Lai, and J.B. Clark Preparation of synaptic and nonsynaptic mitochondria from mammalian brain Methods Enzymol 55 1979 51 60
19. J.C. Lai, and J.B. Clark Isolation and characterization of synaptic and nonsynaptic mitochondria from mammalian brain N.A. Bouiton G.B. Baker Carbohydrates and energy metabolism 1989 Humana Press Clifton 43 98
20. M. McKenna, J. O'Brien, K. Kla, and I.B. Hopkins Differential distribution of LDH isozemes in cortical synaptic terminals and primary cultures of cortical neurons J Neurochem 74 2000 S87
21. M.C. McKenna, J.H. Stevenson, X. Huang, J.T. Tildon, C.L. Zielke, and I.B. Hopkins Mitochondrial malic enzyme activity is much higher in mitochondria from cortical synaptic terminals compared with mitochondria from primary cultures of cortical neurons or cerebellar granule cells Neurochem Int 36 2000 451 459
22. D.G. Nicholls Calcium transport and porton electrochemical potential gradient in mitochondria from guinea-pig cerebral cortex and rat heart Biochem J 170 1978 511 522
23. C.H. Park, E. Carboni, P.L. Wood, and K.W. Gee Characterization of peripheral benzodiazepine type sites in a cultured murine BV-2 microglial cell line Glia 16 1996 65 70
24. J.J. Pysh, and T. Khan Variations in mitochondrial structure and content of neurons and neuroglia in rat brain: an electron microscopic study Brain Res 36 1972 1 18
25. R.V. Raghavendra, A. Dogan, K.K. Bowen, and R.J. Dempsey Traumatic brain injury leads to increased expression of peripheral-type benzodiazepine receptors, neuronal death, and activation of astrocytes and microglia in rat thalamus Exp Neurol 161 2000 102 114
26. R.E. Rosenthal, F. Hamud, G. Fiskum, P.J. Varghese, and S. Sharpe Cerebral ischemia and reperfusion: prevention of brain mitochondrial injury by lidoflazine J Cereb Blood Flow Metab 7 1987 752 758
27. N.R. Sims Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation J Neurochem 55 1990 698 707
28. N.R. Sims, M.F. Anderson, L.M. Hobbs, J.A. Powell, and E. Zaidan Brain mitochondrial responses to postischemic reperfusion: a role for calcium and hydrogen peroxide? Dev Neurosci 22 2000 366 375
29. U. Sonnewald, A. Schousboe, H. Qu, and H.S. Waagepetersen Intracellular metabolic compartmentation assessed by 13C magnetic resonance spectroscopy Neurochem Int 45 2004 305 310
30. A. Stozharov Relationship between metabolic states of liver mitochondria and the G/F-actin ratio Biomed Biochim Acta 44 1985 1287 1294
31. P.G. Sullivan, C. Dube, K. Dorenbos, O. Steward, and T.Z. Baram Mitochondrial uncoupling protein-2 protects the immature brain from excitotoxic neuronal death Ann Neurol 53 2003 711 717
32. Z. Yavin, and E. Yavin Survival and maturation of cerebral neurons on poly(l-lysine) surfaces in the absence of serum Dev Biol 75 1980 454 459
33. H.R. Zielke, J.T. Tildon, M.E. Landry, and S.R. Max Effect of 8-bromo-cAMP and dexamethasone on glutamate metabolism in rat astrocytes Neurochem Res 15 1990 1115 1122