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Volumn 3, Issue 7, 2008, Pages 1228-1239

Isolation of mitochondria from rat brain using Percoll density gradient centrifugation

Author keywords

[No Author keywords available]

Indexed keywords

DIGITONIN; MYELIN;

EID: 50249154155     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.105     Document Type: Article
Times cited : (212)

References (34)
  • 1
    • 0000417081 scopus 로고
    • Intracellular distributi on of enzymes V. Further studies on the distribution of cytochrome c in rat liver homogenates
    • Schneider, W.C. & Hogeboom, G.H. Intracellular distributi on of enzymes V. Further studies on the distribution of cytochrome c in rat liver homogenates. J. Biol. Chem. 183, 123-128 (1950).
    • (1950) J. Biol. Chem , vol.183 , pp. 123-128
    • Schneider, W.C.1    Hogeboom, G.H.2
  • 2
    • 34147143391 scopus 로고    scopus 로고
    • Isolation and subfractionation of mitochondria
    • Pallotti, F. & Lenaz, G. Isolation and subfractionation of mitochondria. Methods Cell Biol. 80, 3-44 (2007).
    • (2007) Methods Cell Biol , vol.80 , pp. 3-44
    • Pallotti, F.1    Lenaz, G.2
  • 3
    • 73049155988 scopus 로고
    • The isolation of nerve endings from brain: An electron-microscopic study of cell fragments derived by homogenization and centrifugation
    • Grey, E.G. & Whittaker, V.P. The isolation of nerve endings from brain: An electron-microscopic study of cell fragments derived by homogenization and centrifugation. J. Anat. 96, 79-89 (1962).
    • (1962) J. Anat , vol.96 , pp. 79-89
    • Grey, E.G.1    Whittaker, V.P.2
  • 4
    • 0014962720 scopus 로고
    • The metabolism of rat brain mitochondria. Preparation and characterization
    • Clark, J.B. & Nicklas, W.J. The metabolism of rat brain mitochondria. Preparation and characterization. J. Biol. Chem. 18, 4724-4731 (1970).
    • (1970) J. Biol. Chem , vol.18 , pp. 4724-4731
    • Clark, J.B.1    Nicklas, W.J.2
  • 6
    • 0025471534 scopus 로고
    • Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation
    • Sims, N.R. Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation. J. Neurochem. 55, 698-707 (1990).
    • (1990) J. Neurochem , vol.55 , pp. 698-707
    • Sims, N.R.1
  • 7
    • 0001766942 scopus 로고
    • Subcellular distribution of the enzymes of the glutamic acid, glutamine and gamma-aminobutyric acid cycles in rat brain
    • Salganicoff, L. & De Robertis, E. Subcellular distribution of the enzymes of the glutamic acid, glutamine and gamma-aminobutyric acid cycles in rat brain. J. Neurochem. 12, 287-309 (1965).
    • (1965) J. Neurochem , vol.12 , pp. 287-309
    • Salganicoff, L.1    De Robertis, E.2
  • 8
    • 0014474635 scopus 로고
    • The heterogeneity of rat brain mitochondria isolated on continuous sucrose gradients
    • Neidle, A., van den Berg, C.J. & Grynbaum, A. The heterogeneity of rat brain mitochondria isolated on continuous sucrose gradients. J. Neurochem. 16, 225-234 (1969).
    • (1969) J. Neurochem , vol.16 , pp. 225-234
    • Neidle, A.1    van den Berg, C.J.2    Grynbaum, A.3
  • 9
    • 50249183167 scopus 로고
    • Isolation from rat brain of mitochondria devoid of glytolytic activity
    • Tanaka, R. & Abood, L.G. Isolation from rat brain of mitochondria devoid of glytolytic activity. J. Neurochem. 10, 571-576 (1963).
    • (1963) J. Neurochem , vol.10 , pp. 571-576
    • Tanaka, R.1    Abood, L.G.2
  • 10
    • 0018338308 scopus 로고
    • Preparation of synaptic and non-synaptic mitochondria from mammalian brain
    • Lai, J.C.K. & Clark, J.B. Preparation of synaptic and non-synaptic mitochondria from mammalian brain. Methods Enzymol. 55, 51-60 (1979).
    • (1979) Methods Enzymol , vol.55 , pp. 51-60
    • Lai, J.C.K.1    Clark, J.B.2
  • 11
    • 0034057202 scopus 로고    scopus 로고
    • Improved recovery of highly enriched mitochondrial fractions from small brain tissue samples
    • Anderson, M.F. & Sims, N.R. Improved recovery of highly enriched mitochondrial fractions from small brain tissue samples. Brain Res. Brain Res. Protoc. 5, 95-101 (2000).
    • (2000) Brain Res. Brain Res. Protoc , vol.5 , pp. 95-101
    • Anderson, M.F.1    Sims, N.R.2
  • 12
    • 0018096805 scopus 로고
    • Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes
    • Elias, P.M., Goerke, J. & Friend, D.S. Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes. J. Cell Biol. 78, 577-596 (1978).
    • (1978) J. Cell Biol , vol.78 , pp. 577-596
    • Elias, P.M.1    Goerke, J.2    Friend, D.S.3
  • 13
    • 0015209679 scopus 로고
    • Enzymic characterization and lipid composition of rat liver subcellular membranes
    • Colbeau, A., Nachbaur, J. & Vignais, P.M. Enzymic characterization and lipid composition of rat liver subcellular membranes. Biochim. Biophys. Acta Biomemb. 249, 462-492 (1971).
    • (1971) Biochim. Biophys. Acta Biomemb , vol.249 , pp. 462-492
    • Colbeau, A.1    Nachbaur, J.2    Vignais, P.M.3
  • 14
    • 0018609465 scopus 로고
    • A method for the rapid separation of soluble and particulate components of rat brain synaptosomes
    • Booth, R.F.G. & Clark, J.B. A method for the rapid separation of soluble and particulate components of rat brain synaptosomes. FEBS Lett. 107, 387-392 (1979).
    • (1979) FEBS Lett , vol.107 , pp. 387-392
    • Booth, R.F.G.1    Clark, J.B.2
  • 15
    • 0016376551 scopus 로고
    • The isolation of outer and inner mitochondrial membranes
    • Greenawalt, J.W. The isolation of outer and inner mitochondrial membranes. Methods Enzymol. 31, 310-323 (1974).
    • (1974) Methods Enzymol , vol.31 , pp. 310-323
    • Greenawalt, J.W.1
  • 16
    • 0037206665 scopus 로고    scopus 로고
    • Calcium-induced cytochrome c release from rat brain mitochondria is altered by digitonin
    • Brustovetsky, N., Jemmerson, R. & Dubinsky, J.M. Calcium-induced cytochrome c release from rat brain mitochondria is altered by digitonin. Neurosci Lett. 332, 91-94 (2002).
    • (2002) Neurosci Lett , vol.332 , pp. 91-94
    • Brustovetsky, N.1    Jemmerson, R.2    Dubinsky, J.M.3
  • 18
    • 30444434747 scopus 로고    scopus 로고
    • Photon correlation spectroscopy of brain mitochondrial populations: Application to traumatic brain injury
    • Lifshitz, J., Janmey, P.A. & McIntosh, T.K. Photon correlation spectroscopy of brain mitochondrial populations: Application to traumatic brain injury. Exp. Neurol. 197, 318-329 (2006).
    • (2006) Exp. Neurol , vol.197 , pp. 318-329
    • Lifshitz, J.1    Janmey, P.A.2    McIntosh, T.K.3
  • 19
    • 36248984867 scopus 로고    scopus 로고
    • Spinal cord mitochondria display lower calcium retention capacity compared with brain mitochondria without inherent differences in sensitivity to cyclophilin D inhibition
    • Morota, S. et al. Spinal cord mitochondria display lower calcium retention capacity compared with brain mitochondria without inherent differences in sensitivity to cyclophilin D inhibition. J. Neurochem. 103, 2066-2076 (2007).
    • (2007) J. Neurochem , vol.103 , pp. 2066-2076
    • Morota, S.1
  • 20
    • 34447498864 scopus 로고    scopus 로고
    • High cyclophilin D content of synaptic mitochondria results in increased vulnerability to permeability transition
    • Naga, K.K., Sullivan, P.G. & Geddes, J.W. High cyclophilin D content of synaptic mitochondria results in increased vulnerability to permeability transition. J. Neurosci. 27, 7469-7475.
    • J. Neurosci , vol.27 , pp. 7469-7475
    • Naga, K.K.1    Sullivan, P.G.2    Geddes, J.W.3
  • 21
    • 0025075050 scopus 로고
    • Halothane impairs the bioenergetic functions of isolated rat liver mitochondria
    • Tsuchiya, M., Takahashi, M., Tomoda, M., Ueda, W. & Hirakawa, M. Halothane impairs the bioenergetic functions of isolated rat liver mitochondria. Toxicol. Appl. Pharmacol. 104, 466-475 (1990).
    • (1990) Toxicol. Appl. Pharmacol , vol.104 , pp. 466-475
    • Tsuchiya, M.1    Takahashi, M.2    Tomoda, M.3    Ueda, W.4    Hirakawa, M.5
  • 22
    • 0028997104 scopus 로고
    • Reduced mitochondrial respiration in mouse cerebral cortex during chronic hypoxia
    • Chávez, J.C., Pichiule, P., Boero, J. & Arregui, A. Reduced mitochondrial respiration in mouse cerebral cortex during chronic hypoxia. Neurosci. Lett. 193, 169-172 (1995).
    • (1995) Neurosci. Lett , vol.193 , pp. 169-172
    • Chávez, J.C.1    Pichiule, P.2    Boero, J.3    Arregui, A.4
  • 23
    • 33947200596 scopus 로고    scopus 로고
    • Mitochondrial dysfunction in Hungtington's disease: The bioenergetics of isolated and in situ mitochondria from transgenic mice
    • Oliveira, J.M.A. et al. Mitochondrial dysfunction in Hungtington's disease: The bioenergetics of isolated and in situ mitochondria from transgenic mice. J. Neurochem. 101, 241-249 (2007).
    • (2007) J. Neurochem , vol.101 , pp. 241-249
    • Oliveira, J.M.A.1
  • 24
    • 33846607750 scopus 로고    scopus 로고
    • Expression, microsomal and mitochondrial activities of cytochrome P450 enzymes in brain regions from control and phenobarbital-treated rabbits
    • Marini, S. et al. Expression, microsomal and mitochondrial activities of cytochrome P450 enzymes in brain regions from control and phenobarbital-treated rabbits. Life Sci. 80, 910-917 (2007).
    • (2007) Life Sci , vol.80 , pp. 910-917
    • Marini, S.1
  • 25
    • 33750323814 scopus 로고    scopus 로고
    • In vivo administration of D609 leads to protection of subsequently isolated gerbil brain mitochondria subjected to in vitro oxidative stress induced by amyloid beta-peptide and other oxidative stressors: Relevance to ALzheimer's disease and other oxidative stress-related neurodegenerative disorders
    • Ansari, M.A. et al. In vivo administration of D609 leads to protection of subsequently isolated gerbil brain mitochondria subjected to in vitro oxidative stress induced by amyloid beta-peptide and other oxidative stressors: Relevance to ALzheimer's disease and other oxidative stress-related neurodegenerative disorders. Free Radic. Biol. Med. 41, 1694-1703 (2006).
    • (2006) Free Radic. Biol. Med , vol.41 , pp. 1694-1703
    • Ansari, M.A.1
  • 26
    • 0033972055 scopus 로고    scopus 로고
    • Multiple forms of cytochrome P450 and associated monooxygenase activities in human brain mitochondria
    • Bhagwat, S.V., Boyd, M.R. & Ravindranath, V. Multiple forms of cytochrome P450 and associated monooxygenase activities in human brain mitochondria. Biochem. Pharmacol. 59, 573-582 (2000).
    • (2000) Biochem. Pharmacol , vol.59 , pp. 573-582
    • Bhagwat, S.V.1    Boyd, M.R.2    Ravindranath, V.3
  • 27
    • 0035217570 scopus 로고    scopus 로고
    • Isolation and characterization of intact mitochondria from neonatal rat brain
    • Rajapaske, N., Shimizu, K., Payne, M. & Busija, D. Isolation and characterization of intact mitochondria from neonatal rat brain. Brain Res. Brain Res. Protoc. 8, 176-183 (2001).
    • (2001) Brain Res. Brain Res. Protoc , vol.8 , pp. 176-183
    • Rajapaske, N.1    Shimizu, K.2    Payne, M.3    Busija, D.4
  • 29
    • 33846022729 scopus 로고    scopus 로고
    • Calcium-dependent spontaneously reversible remodelling of brain mitochondria
    • Shalbuyeva, N., Brustovetsky, T., Bolshakov, A. & Brustovetsky, N. Calcium-dependent spontaneously reversible remodelling of brain mitochondria. J. Biol. Chem. 281, 37547-37558 (2006).
    • (2006) J. Biol. Chem , vol.281 , pp. 37547-37558
    • Shalbuyeva, N.1    Brustovetsky, T.2    Bolshakov, A.3    Brustovetsky, N.4
  • 30
    • 33644781684 scopus 로고    scopus 로고
    • Isolation of mitochondria with high respiratory control from primary cultures of neurons and astrocytes using nitrogen cavitation
    • Kristian, T., Hopkins, I.B., McKenna, M.C. & Fiskum, G. Isolation of mitochondria with high respiratory control from primary cultures of neurons and astrocytes using nitrogen cavitation. J. Neurosci. Meth. 152, 136-143.
    • J. Neurosci. Meth , vol.152 , pp. 136-143
    • Kristian, T.1    Hopkins, I.B.2    McKenna, M.C.3    Fiskum, G.4
  • 31
    • 0026010158 scopus 로고
    • Selective impairment of respiration in mitochondria isolated from brain subregions following transient forebrain ischemia in the rat
    • Sims, N.R. Selective impairment of respiration in mitochondria isolated from brain subregions following transient forebrain ischemia in the rat. J. Neurochem. 56, 1836-1844 (1991).
    • (1991) J. Neurochem , vol.56 , pp. 1836-1844
    • Sims, N.R.1
  • 32
    • 0022499110 scopus 로고
    • Expression of classical mitochondrial respiratory responses in homogenates of rat forebrain
    • Sims, N.R. & Blass, J.P. Expression of classical mitochondrial respiratory responses in homogenates of rat forebrain. J. Neurochem. 47, 496-505 (1986).
    • (1986) J. Neurochem , vol.47 , pp. 496-505
    • Sims, N.R.1    Blass, J.P.2
  • 33
    • 0001272626 scopus 로고
    • The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations
    • Johnson, M.K. The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations. Biochem. J. 77, 610-618 (1960).
    • (1960) Biochem. J , vol.77 , pp. 610-618
    • Johnson, M.K.1
  • 34
    • 0017091352 scopus 로고
    • A rapid assay for 2′, 3′-cyclic nucleotide 3′-phosphohydrolase
    • Sims, N.R. & Carnegie, P.R. A rapid assay for 2′, 3′-cyclic nucleotide 3′-phosphohydrolase. J. Neurochem. 27, 769-772 (1976).
    • (1976) J. Neurochem , vol.27 , pp. 769-772
    • Sims, N.R.1    Carnegie, P.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.