The key role of a non-active-site residue Met148 on the catalytic efficiency of meta-cleavage product hydrolase BphD

Applied Microbiology and Biotechnology

Volumn 97, Issue 24, 2013, Pages 10399-10411

  Zhou, Hao ^a   Qu, Yuanyuan ^a   Kong, Chunlei ^a   Shen, E ^a   Wang, Jingwei ^a   Zhang, Xuwang ^a   Ma, Qiao ^a   Zhou, Jiti ^a  

^a DALIAN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Meta cleavage product hydrolase; Molecular dynamics; Saturation mutagenesis; Tunnel analysis

Indexed keywords

ACTIVE SITE RESIDUES; CATALYTIC EFFICIENCIES; META-CLEAVAGE; MOLECULAR DYNAMICS SIMULATIONS; SATURATION MUTAGENESIS; SITE DIRECTED MUTAGENESIS; STRUCTURE-FUNCTION RELATIONSHIP; TRANSIENT KINETIC ANALYSIS;

CLUSTER ANALYSIS; EFFICIENCY; MOLECULAR DYNAMICS; MUTAGENESIS;

HYDROLASES;

6 OXO 6 PHENYLHEXA 2,4 DIENOIC ACID; CHEMICAL COMPOUND; ENZYME; HYDROLASE; MET 148 ENZYME; META CLEAVAGE PRODUCT HYDROLASE; UNCLASSIFIED DRUG;

BIODEGRADATION; CATALYSIS; CHEMICAL BINDING; ENZYME ACTIVITY; MOLECULAR ANALYSIS; MUTAGENICITY; OXIC CONDITIONS; REACTION KINETICS;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CLUSTER ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME KINETICS; HYDROGEN BOND; MOLECULAR DYNAMICS; PROTEIN INTERACTION; RELAXATION TIME; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; STEADY STATE; STRUCTURE ACTIVITY RELATION;

DNA MUTATIONAL ANALYSIS; FATTY ACIDS, UNSATURATED; HYDROLASES; KINETICS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION, MISSENSE;

EID: 84892369388     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4814-0     Document Type: Article

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