High-level secretory production of recombinant single-chain variable fragment (scFv) in Corynebacterium glutamicum

Applied Microbiology and Biotechnology

Volumn 98, Issue 1, 2014, Pages 273-284

  Yim, Sung Sun ^a   An, Seul Ji ^a   Choi, Jae Woong ^a   Ryu, Ae Jin ^a   Jeong, Ki Jun ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Antibody fragment; Corynebacterium glutamicum; Fed batch cultivation; Secretion

Indexed keywords

ENZYME ACTIVITY; PEPTIDES; PURIFICATION;

ANTIBODY FRAGMENT; CORYNEBACTERIUM GLUTAMICUM; ENZYME LINKED IMMUNOSORBENT ASSAY; FED-BATCH CULTIVATION; LARGE SCALE PRODUCTIONS; PURIFICATION PROCEDURES; SECRETION; SINGLE CHAIN VARIABLE FRAGMENTS;

ANTIBODIES;

ANTHRAX TOXIN; SIGNAL PEPTIDE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TOXIN ANTIBODY;

ANTIBODY; BIOREACTOR; CONCENTRATION (COMPOSITION); CULTIVATION; GENETIC ANALYSIS; GROWTH RATE; MICROBIAL ACTIVITY; OPTIMIZATION; PEPTIDE; SECRETION;

5' UNTRANSLATED REGION; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BIOREACTOR; CELL GROWTH; CODON USAGE; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; CULTURE MEDIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; FED BATCH CULTURE; FED BATCH FERMENTATION; GENE EXPRESSION SYSTEM; GENE SEQUENCE; NONHUMAN; PROMOTER REGION; PURIFICATION; RNA SEQUENCE; TRANSCRIPTION TERMINATION;

CORYNEBACTERIUM GLUTAMICUM;

ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BACTERIAL TOXINS; CORYNEBACTERIUM GLUTAMICUM; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SINGLE-CHAIN ANTIBODIES;

EID: 84891853096     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5315-x     Document Type: Article

References (40)

1. Ahmad ZA, Yeap SK, Ali AM, Ho WY, Alitheen NB, Hamid M (2012) scFv antibody: principles and clinical application. Clin Dev Immunol 2012:980250
2. Aldor IS, Krawitz DC, Forrest W, Chen C, Nishihara JC, Joly JC, Champion KM (2005) Proteomic profiling of recombinant Escherichia coli in high-cell-density fermentations for improved production of an antibody fragment biopharmaceutical. Appl Environ Microbiol 71(4):1717-1728
3. An SJ, Yim SS, Jeong KJ (2013) Development of a secretion system for the production of heterologous proteins in Corynebacterium glutamicum using the Porin B signal peptide. Protein Expr Purif 89:251-257
4. Buss NA, Henderson SJ, Mcfarlane M, Shenton JM, de Haan L (2012) Monoclonal antibody therapeutics: history and future. Curr Opin Pharmacol 12:615-622
5. Chen R (2012) Bacterial expression systems for recombinant protein production: E. coli and beyond. Biotechnol Adv 30:1102-1107
6. Dammeyer T, Steinwand M, Krüger SC, Dübel S, Hust M, Timmis KN (2011) Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. Microb Cell Fact 10:11
7. Date M, Itaya H, Matsui H, Kikuchi Y (2006) Secretion of human epidermal growth factor by Corynebacterium glutamicum. Lett Appl Microbiol 42(1):66-70
8. Eikmanns BJ (1992) Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and thiosephosphate isomerase. J Bacteriol 174(19):6076-6086
9. Ermolaeva MD (2001) Synonymous codon usage in bacteria. Curr Issues Mol Biol 3(4):91-97
10. Harvey BR, Georgiou G, Hayhurst A, Jeong KJ, Iverson BL, Rogers GK (2004) Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. Proc Natl Acad Sci U S A 101:9193-9198
11. Hong HJ, Kim ST (2002) Antibody engineering. Biotechnol Bioprocess Eng 7:150-154
12. Huang CJ, Lin H, Yang X (2012) Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements. J Ind Microbiol Biotechnol 39(3):383-399
13. Jang SH, Jeong KJ (2013) Overproduction of a C5a receptor antagonist (C5aRA) in Escherichia coli. Protein Expr Purif 89(2):169-174
14. Jeong KJ, Jang SH, Velmurugan N (2011) Recombinant antibodies: engineering and production in yeast and bacterial hosts. Biotechnol J 6(1):16-27
15. Jiang L, He L, Fountoulakis M (2004) Comparison of protein precipitation methods for sample preparation prior to proteomic analysis. J Chromatogr A 1023:317-320
16. Jo SJ, Matsumoto K, Leong CR, Ooi T, Taguchi S (2007) Improvement of poly(3-hydroxybutyrate) [P(3HB)] production in Corynebacterium glutamicum by codon optimization, point mutation and gene dosage of P(3HP) biosynthetic genes. J Biosci Bioeng 104(6):457-463
17. Katsuda T, Sonoda H, Kumada Y, Yamaji H (2012) Production of antibody fragments in Escherichia coli. Methods Mol Biol 907:305-324
18. Kikuchi Y, Date M, Yokoyama K, Umezawa Y, Matsui H (2003) Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-like protease from Streptomyces albogriseolus. Appl Environ Microbiol 69(1):358-366
19. Kikuchi Y, Itaya H, Date M, Matsui K, Wu LF (2009) TatABC overexpression improves Corynebacterium glutamicum Tat-dependent protein secretion. Appl Environ Microbiol 75:603-607
20. Kind S, Jeong WK, Schröder H, Wittmann C (2010) Systems-wide metabolic pathway engineering in Corynebacterium glutamicum for bio-based production of diaminopentane. Metab Eng 12(4):341-351
21. Lee YJ, Jeong KJ (2013) Enhanced production of antibody fragment via SRP pathway engineering in Escherichia coli. Biotechnol Bioprocess Eng 18:751-758
22. Lee YJ, Kim HS, Ryu AJ, Jeong KJ (2013) Enhanced production of full-length immunoglobulin G via the signal recognition particle (SRP)-dependent pathway in Escherichia coli. J Biotechnol 165:102-108
23. Liu G, Wu J, Yang H, Bao Q (2010) Codon usage patterns in Corynebacterium glutamicum: mutational bias, natural selection and amino acid conservation. Comp Funct Genomics 2010:343569
24. Lv Y, Wu Z, Han S, Lin Y, Zheng S (2012) Construction of recombinant Corynebacterium glutamicum for l-threonine production. Biotechnol Bioprocess Eng 17(1):16-21
25. Malumbres M, Gil JA, Martin JF (1993) Codon preference in Corynebacteria. Gene 134(1):15-24
26. Matsuda Y, Itaya H, Kikuchi Y, Beppu H, Jomantas JAV, Kutukova EA (2013) Methods for secretory production of protein. WO/2013/065839
27. Mutalik VK, Guimaraes JC, Cambray G, Mai QA, Christoffersen MJ, Marin L, Yu A, Lam C, Rodriguez C, Bennett G, Keasling JD, Endy D, Arkin AP (2013) Quantitative estimation of activity and quality for collections of functional genetic elements. Nat Methods 10(4):347-353
28. Neuner A, Heinzle E (2011) Mixed glucose and lactate uptake by Corynebacterium glutamicum through metabolic engineering. Biotehcnol J 6(3):318-329
29. Park JU, Jo JH, Kim YJ, Chung SS, Lee JH, Lee HH (2008) Construction of heat-inducible expression vector of Corynebacterium glutamicum and C. ammoniagenes: fusion of lambda operator with promoters isolated from C. ammoniagenes. J Microbiol Biotechnol 18(4):639-647
30. Pátek M, Nešvera J (2013) Promoters and plasmid vectors of Corynebacterium glutamicum. In: Yukawa H, Inui M (eds) Corynebacterium glutamicum: biology and biotechnology. Springer, Heidelberg, pp 51-88
31. Persson J, Lester P (2004) Purification of antibody and antibody fragment from E. coli homogenate using 6,9-diamino-2-ethoxyacridine lactate as precipitation agent. Biotechnol Bioeng 87(3):424-434
32. Pfleger BF, Pitera DJ, Smolke CD, Keasling JD (2006) Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol 24:1027-1032
33. Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
34. Sundaram RK, Hurwitz I, Matthews S, Hoy E, Kurapati S, Crawford C, Sundaram P, Durvasula RV (2008) Expression of a functional single-chain antibody via Corynebacterium pseudodiphtheriticum. Eur J Clin Microbiol Infect Dis 27:617-622
35. Teramoto H, Watanabe K, Suzuki N, Inui M, Yukawa H (2011) High yield secretion of heterologous proteins in Corynebacterium glutamicum using its own Tat-type signal sequence. Appl Microbiol Biotechnol 91:677-687
36. Vertès AA (2013) Protein secretion systems of Corynebacterium glutamicum. In: Yukawa H, Inui M (eds) Corynebacterium glutamicum: biology and biotechnology. Springer, Heidelberg, pp 351-389
37. Watanabe K, Teramoto H, Suzuki N, Inui M, Yukawa H (2013) Influence of SigB inactivation on Corynebacterium glutamicum protein secretion. Appl Microbiol Biotechnol 97(11):4917-4926
38. Wendisch VF, Bott M, Kalinowski J, Oldiges M, Wiechert W (2006) Emerging Corynebacterium glutamicum systems biology. J Biotechnol 123(1):74-92
39. Westers L, Westers H, Quax WJ (2004) Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim Biophys Acta 1694(1):299-310
40. Yim SS, An SJ, Kang M, Lee J, Jeong KJ (2013) Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum. Biotechnol Bioeng 110(11):2959-2971