Overproduction of a C5a receptor antagonist (C5aRA) in Escherichia coli

Protein Expression and Purification

Volumn 89, Issue 2, 2013, Pages 169-174

  Jang, Seung Hoon ^a   Jeong, Ki Jun ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Batch fermentation; C5aR antagonist; Escherichia coli; NusA

Indexed keywords

COMPLEMENT COMPONENT C5A RECEPTOR; ELONGATION FACTOR; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; NUSA PROTEIN, E COLI; THIOREDOXIN; TRANSCRIPTION FACTOR;

ARTICLE; BIOREACTOR; CHEMISTRY; DRUG ANTAGONISM; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PLASMID; SOLUBILITY;

BIOREACTORS; CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENETIC VECTORS; HUMANS; MALTOSE-BINDING PROTEINS; PEPTIDE ELONGATION FACTORS; PLASMIDS; RECEPTOR, ANAPHYLATOXIN C5A; RECOMBINANT FUSION PROTEINS; SOLUBILITY; THIOREDOXINS; TRANSCRIPTION FACTORS;

ESCHERICHIA COLI;

EID: 84876249941     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.03.004     Document Type: Article

References (20)

1. T.M. Woodruff, K.S. Nandakumar, and F. Tedesco Inhibiting the C5-C5a receptor axis Mol. Immunol. 48 2011 1631 1642
2. D. Ricklin, G. Hajishengallis, K. Yang, and J.D. Lambris Complement: a key system for immune surveillance and homeostasis Nat. Immunol. 11 2010 785 797
3. D.G. Nettesheim, R.P. Edalji, K.W. Mollison, J. Greer, and E.R. Zuiderweg Secondary structure of complement component C3a anaphylatoxin in solution as determined by NMR spectroscopy: differences between crystal and solution conformations Proc. Natl. Acad. Sci. USA 85 1988 5036 5040
4. A. Klos, A.J. Tenner, K.O. Johswich, R.R. Ager, E.S. Reis, and J. Kohl The role of the anaphylatoxins in health and disease Mol. Immunol. 46 2009 2753 2766
5. R.F. Guo, and P.A. Ward Role of C5a in inflammatory responses Annu. Rev. Immunol. 23 2005 821 852 (Pubitemid 40563186)
6. J. Kohl Anaphylatoxins and infectious and non-infectious inflammatory diseases Mol. Immunol. 38 2001 175 187 (Pubitemid 32786775)
7. T. Heller, M. Hennecke, U. Baumann, J.E. Gessner, A.M. zu Vilsendorf, M. Baensch, F. Boulay, A. Kola, A. Klos, W. Bautsch, and J. Kohl Selection of a C5a receptor antagonist from phage libraries attenuating the inflammatory response in immune complex disease and ischemia/reperfusion injury J. Immunol. 163 1999 985 994 (Pubitemid 29328111)
8. J. Sambrook, and D. Russell Molecular Cloning: A Laboratory Manual third ed. 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
9. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
10. S.Y. Lee High cell-density culture of Escherichia coli Trends Biotechnol. 14 1996 98 105 (Pubitemid 26079509)
11. S.H. Jang, C.H. Lee, Y.S. Kim, and K.J. Jeong High-level production of a kringle domain variant by high-cell-density cultivation of Escherichia coli Appl. Microbiol. Biotechnol. 92 2011 327 336
12. K.J. Jeong, and M. Rani High-level production of a single chain antibody against anthrax toxin in Escherichia coli by high cell density cultivation Bioprocess Biosyst. Eng. 34 2011 811 817
13. H. Kadokura, F. Katzen, and J. Beckwith Protein disulfide bond formation in prokaryotes Annu. Rev. Biochem. 72 2003 111 135 (Pubitemid 36930443)
14. K.J. Jeong, S.H. Jang, and N. Velmurugan Recombinant antibodies: engineering and production in yeast and bacterial hosts Biotechnol. J. 6 2011 16 27
15. D. Liu, R.D. Schmid, and M. Rusnak Functional expression of Candida Antarctica lipase B in the Escherichia coli cytoplasm - a screening system for a frequently used biocatalyst Appl. Microbiol. Biotechnol. 72 2006 1024 1032 (Pubitemid 44477381)
16. A. Neubauer, J. Soini, M. Bollok, M. Zenker, J. Sandqvist, J. Myllyharju, and P. Neubauer Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline J. Biotechnol. 128 2007 308 321 (Pubitemid 46074202)
17. M.W. Larsen, U.T. Bornscheuer, and K. Hult Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems Protein Expr. Purif. 62 2008 90 97
18. L.M. MacDonald, A. Armson, R.C. Thompson, and J.A. Reynoldson Characterization of factors favoring the expression of soluble protozoan tubulin proteins in Escherichia coli Protein Expr. Purif. 29 2003 117 122 (Pubitemid 36599801)
19. A. Jacquet, V. Daminet, M. Haumont, L. Garcia, S. Chaudoir, A. Bollen, and R. Biemans Expression of a recombinant Toxoplasma gondii ROP2 fragment as a fusion protein in bacteria circumvents insolubility and proteolytic degradation Protein Expr. Purif. 17 1999 392 400
20. J.G. Marblestone, S.C. Edavettal, Y. Lim, P. Lim, X. Zuo, and T.R. Butt Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO Protein Sci. 15 2006 182 189 (Pubitemid 43004245)